MG1 Maraba/MAGE-A3, With and Without Adenovirus Vaccine With Transgenic MAGE-A3 Insertion in Incurable MAGE-A3-Expressing Solid Tumours

A Phase I/II Study of MG1 Maraba/MAGE-A3 (MG1MA3), With and Without Adenovirus Vaccine, With Transgenic MAGE-A3 Insertion (AdMA3) in Patients With Incurable Advanced/Metastatic MAGE-A3-Expressing Solid Tumours

This research is being done because these viruses have been shown to shrink tumours in animals and human tumour samples by selectively killing cancer cells and creating an immune response to the tumour antigen contained in the viruses. This effect has been shown to increase when the AdMA3 virus is given first. It is not clear if this treatment will offer better results than standard treatment.

Study Overview

• Status: Active, not recruiting
• Conditions: Advanced/Metastatic Solid Tumours
• Intervention / Treatment: Biological: MG1MA3; Biological: AdMA3

Detailed Description

The purpose of the first phase of this study (phase I) is to find the dose of a new therapy, the MG1 Maraba/MAGE-A3 (MG1MA3) virus that can be given alone and in combination with the Adenovirus/MAGE-A3 (AdMA3) virus. In the first part of the study, patients may receive the Maraba virus, the Adenovirus or both viruses. To identify the highest safe dose of the Maraba virus alone or in combination the study will start at a dose lower than the one that does not cause side effects in animals. Participants are given one or both of these therapies and are watched very closely to see what side effects they have and to make sure the side effects are not severe. If serious side effects are seen in patients at the first dose level, doses of MG1MA3 may be lowered in subsequent patients. If the side effects are not serious, then more potential participants are asked to join this study and are given higher doses. This will continue until the maximum feasible dose level is reached or one of the lower doses is found that causes serious but temporary side effects. Doses higher than that will not be given.

• Study Type: Interventional
• Enrollment (Actual): 56
• Phase: Phase 2; Phase 1

Contacts and Locations

Study Locations

• Canada
  • British Columbia
    • Vancouver, British Columbia, Canada, V5Z 4E6
      • BCCA - Vancouver Cancer Centre
  • Ontario
    • Hamilton, Ontario, Canada, L8V 5C2
      • Juravinski Cancer Centre at Hamilton Health Sciences
    • Ottawa, Ontario, Canada, K1H 8L6
      • Ottawa Hospital Research Institute
    • Toronto, Ontario, Canada, M5G 2M9
      • University Health Network

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years and older (Adult, Older Adult)
• Accepts Healthy Volunteers: No

Description

Inclusion Criteria:

• PHASE I: Patients must have histologically confirmed, unresectable locally advanced/metastatic solid tumour with positive expression of MAGE-A3 (primary or metastatic lesion) and for which there is no known life prolonging standard therapy.

• PHASE II: Patients must have histologically confirmed, unresectable locally advanced/metastatic solid tumour with positive expression of MAGE-A3 (primary or metastatic lesion) as follows:

  1. Non-small cell lung cancer (NSCLC) specifically adenocarcinoma and squamous cell carcinoma.
  2. Breast cancer
  3. Esophageal/GEJ cancer/gastric
• In phase II patients may be treated before refractory, such as while stable post treatment response to first line therapy.

• Presence of clinically and/or radiologically documented disease. At least one site of disease must be unidimensionally measurable by CT with IV contrast as follows:

  • Chest x-ray ≥ 20 mm
  • CT scan (with slice thickness of ≤ 5 mm) ≥ 10 mm-->Longest diameter
  • Physical exam (using calipers) ≥ 10 mm
  • Lymph nodes by CT scan ≥ 15 mm -->Measured in short axis
• All radiology studies must be performed within 14 days prior to registration (within 21 days if negative).
• Patients must have at least one additional tumour mass amenable to core needle or excisional biopsy (Note FNA is not acceptable) that is not a measurable lesion that will be used as a target lesion. Patients must consent to and be willing and able to undergo at least two core needle biopsies of that lesion.
• Age ≥ 18 years
• ECOG performance status of 0 or 1
• Patients must have received at least one prior standard first line regimen for advanced or metastatic disease. The regimen may have been cytotoxic chemotherapy, targeted therapy, hormonal therapy (for e.g. anastrozole) or immunotherapy providing considered a standard first line therapy. There is no limit to the number of prior regimens but investigators and their patients should carefully consider the likelihood of benefit of an immunologic therapy in heavily pretreated patients.

• For phase II, patients may be enrolled prior to disease progression, provided they have completed their first line therapy as below and they have documented stable disease on two consecutive tumour assessments (i.e. do not have evidence of tumour regression from therapy):

  • NSCLC patients may be entered after a minimum of 4-6 cycles of first line combination chemotherapy; if the patient is >70 years a single agent regimen is acceptable. If the patient has documented EGFR or ALK mutations, treatment they may have received may include an EGFR inhibitor or ALK inhibitor as first line therapy.
  • Breast cancer patients may be entered after a minimum of 6 cycles of first line therapy.
  • Patients with metastatic/recurrent esophageal carcinoma may be entered after first line chemotherapy for metastatic disease.

• Washout period between last day of prior treatment and planned start of treatment is the longest of one of the following:

  • two weeks
  • standard cycle length of prior regimen
  • 10 half-lives for investigational drugs.
  • 30 days since last dose of ipilumumab or PR1/PDL1 therapy.
• Patients must have recovered from any treatment related toxicities prior to registration (unless grade 1, irreversible and considered not clinically significant). Progression must be documented post radiotherapy if was given to the only site of measurable disease.
• Patients may have had prior radiation therapy. A minimum of 28 days (4 weeks) must have elapsed between the last dose of radiation and date of registration (14 days for a single palliative fraction of radiation to a non-target lesion). Patients must have recovered from any acute toxic effects from radiation prior to registration (unless grade 1, irreversible and considered not clinically significant).
• Previous surgery is permitted. A minimum of 28 days (4 weeks) must have elapsed between any major surgery and date of registration (7 days for minor surgery), provided that wound healing has occurred.

• Laboratory requirements done within 7 days prior to registration:

  • WBC ≥ 3.0 x 10^9/L
  • absolute neutrophils ≥ 1.5 x 10^9/L
  • platelets ≥ 75 x 10^9/L
  • INR ≤ 1.2
  • bilirubin ≤ 1.5 x UNL (upper normal limit)
  • AST and ALT ≤ 3.0 x UNL or ≤ 5.0 x UNL if patient has liver metastases
  • serum creatinine ≤ 1.5 x UNL or creatinine clearance ≥ 60ml/min
  • serum phosphate > 0.8mMol/L (grade 0-1)
• Patient consent must be appropriately obtained in accordance with applicable local and regulatory requirements. Each patient must sign a consent form prior to registration and prior to tests which are considered to be study specific
• Patients who cannot give informed consent (i.e. mentally incompetent patients, or those physically incapacitated such as comatose patients) are not to be recruited into the study. Patients competent but physically unable to sign the consent form may have the document signed by their nearest relative or legal guardian. Each patient will be provided with a full explanation of the study before consent is requested.
• Patients must be accessible for treatment and follow up. Patients registered on this trial must be treated and followed at the participating centre.
• Pre-treatment biopsy must be done within 5 working days after registration and treatment is to begin within 5 working days of the pre-treatment biopsy (max. 10 working days from registration).

Exclusion Criteria:

• Patients with a history of other active or current malignancies that require active treatment
• Patients with known symptomatic brain metastases. Patients with treated and radiologic or clinical evidence of stable brain metastases, are eligible providing that they have been stable for at least 3 months, are asymptomatic and do not require corticosteroids (must have discontinued steroids at least 1 month prior to entry).
• Patients receiving concurrent treatment with other anti-cancer therapy or other investigational agents.
• Patients who have had prior treatment with AdVAC, MG1MA3 or any MAGE-A3 targeted therapy.
• Pregnant or lactating women. Men and women of childbearing potential who do not agree to use adequate contraception (hormonal or barrier method of birth control; abstinence) prior to study entry and for the duration of the study participation.

• Serious illness or medical condition which would not permit the patient to be managed according to the protocol, including, but not limited to:

  1. History of significant neurologic or psychiatric disorder (e.g. uncontrolled psychotic disorders) which would impair the ability to obtain consent or limit compliance with study requirements.
  2. Active uncontrolled or serious infection (viral, bacterial or fungal) or a history of opportunistic infection associated with an immunodeficient state.
  3. Significant immunodeficiency due to underlying illness (e.g. known HIV/AIDS) and/or medication (e.g. systemic corticosteroids).
  4. Known myeloproliferative disorders requiring systemic therapy.
  5. Other medical conditions that might be aggravated by study treatment.
• Patients with uncontrolled pre-existing cardiovascular conditions and/or symptomatic cardiac dysfunction.

• Patients with household contacts meeting any of the following criteria are ineligible for study entry unless alternate living arrangements can be made:

  1. Women who are pregnant or nursing an infant
  2. Children < 12 months old
  3. Anyone with significant immunodeficiency due to underlying illness (e.g. HIV/AIDS) and/or medication (e.g. systemic corticosteroids)
• Use of anti-viral medication, steroids, immunosuppressive agents (cyclosporine, interferon) or immunization (including the flu shot) within 14 days prior to registration. Use of anti-viral, anti-platelet, or anti-coagulation medication that cannot be discontinued within 14 days of enrollment.
• Patients with disease/tumour invading a major vascular structure (e.g. carotid artery), tumour related impending bowel obstruction or clinically significant and/or rapidly accumulating ascites, pericardial or pleural effusions.
• Patients with conditions likely to have resulted in splenic dysfunction (e.g. splenectomy, sickle cell anemia, radiation to the spleen ≥ 20Gy, congenital asplenism).
• Patients with ≥ grade 2 dyspnea and/or requirement for supplemental oxygen. Patients with important pulmonary disease must complete a 6 minute ambulation test with O2 states ≥ 90% to be eligible

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: Non-Randomized
• Interventional Model: Parallel Assignment
• Masking: None (Open Label)

Number of Arms

3

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Arm A (MG1MA3 virus alone); The starting dose of MG1MA3 will be 1 x 10^10 pfu administered by IV on day 1 and day 4.MG1MA3 dose will be escalated as per protocol.	Biological: MG1MA3; MG1MA3: Boosting component of Oncolytic Vaccine; Other Names: MG1 Maraba/MAGE-A3
Experimental: Arm B- AdMA3 (vaccine prime) alone; Six patients will receive prime AdMA3 vaccine at a dose of 1x10^10 pfu administered IM on day (-14). No dose escalation is planned.	Biological: AdMA3; AdMA3: Priming component of Oncolytic Vaccine; Other Names: Adenovirus/MAGE-A3
Experimental: Arm C- AdMA3 plus MG1MA3 (prime + boost); Prime AdMA3 vaccine will be administered as a single dose of 1x10^10 pfu IM at day (-14) followed by dose escalation of MG1MA3 boost, IV administered on days 1 & 4 at a starting dose of 1 log below the recommended phase II dose (RP2D), as determined in Arm A of this study. MG1MA3 dose will be escalated as defined in protocol.	Biological: MG1MA3; MG1MA3: Boosting component of Oncolytic Vaccine; Other Names: MG1 Maraba/MAGE-A3; Biological: AdMA3; AdMA3: Priming component of Oncolytic Vaccine; Other Names: Adenovirus/MAGE-A3

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Phase I: Toxicity as measured by adverse events	To Determine maximum feasible dose (MFD) of: MG1MA3 when administered alone on day 1 & day 4 (Arm A); MG1MA3 when administered on day 1 & 4 or days 1, 4, 8 &11 following immunologic priming with AdMA3 (Arm C); To confirm the safety profile of AdMA3 administration (Arm B).	3 years
Phase II: Objective tumour response rate (ORR) using RECIST v1.1.	To evaluate the objective tumour response rate (ORR) using RECIST v1.1.	16 weeks

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Phase I: Number and Severity of Adverse Events in patients	To determine the safety profile of: MG1MA3 when administered alone (Arm A);; MG1MA3 when administered on day 1 & 4 or days 1, 4, 8 &11 following immunologic priming with AdMA3 (Arm C). Adverse events will be monitored on an ongoing basis by the central office and their frequencies reported semi-annually at investigators' meetings. Toxic effects will be categorized using the CTCAE. The worst event for each patient in each category or subcategory will be described. Both events related and unrelated to treatment will be captured.	8 weeks
Phase I: MG1MA3 clearance and secondary replication from pharmacokinetics and viral shedding	To determine the pharmacokinetics, including viral shedding, of MG1MA3 when administered: Alone on day 1 & day 4 (Arm A);; Following immunologic priming with AdMA3 (Arm C). Pharmacokinetic parameters including MG1MA3 clearance and secondary replication (genomes and infectious units), will be evaluated in blood over time and summarized descriptively by treatment arms in phase I portion.	3 years
Phase I: Delivery to, and viral detection and replication within, tumours for MG1MA3	To determine the delivery to, and viral detection and replication within, tumours for MG1MA3 when administered: Alone on day 1 & day 4 (Arm A);; Following immunologic priming with AdMA3 (Arm C).	3 years
Phase I: Cellular and humoral immune response to virus and tumour antigens	To determine the cellular and humoral immune response to virus and tumour antigens (for all arms).	3 years
Phase I: Efficacy using RECIST v1.1 and iRECIST	To evaluate preliminary evidence of efficacy using RECIST v1.1 and iRECIST	3 years
Phase II: pharmacokinetics (PK) of MG1MA3 (MG1MA3 clearance and secondary replication (genomes and infectious units)	To further explore the pharmacokinetics (PK) of MG1MA3. Pharmacokinetic parameters including MG1MA3 clearance and secondary replication (genomes and infectious units), will be evaluated in blood over time and summarized descriptively by tumour types in phase II portion.	3 years
Phase II: cellular and humoral immune response to virus and tumour antigens	To further evaluate the cellular and humoral immune response to virus and tumour antigens.	16 weeks
Phase II: toxicity as measured by adverse events of MG1MA3 following AdMA3	To further explore the safety profile of MG1MA3 following AdMA3	3 years
Phase II: Response by iRECIST	To evaluate response by iRECIST	16 weeks

Collaborators and Investigators

• Sponsor: Canadian Cancer Trials Group
• Collaborators: Ottawa Hospital Research Institute
• Investigators: Study Chair: Derek Jonker, Ottawa Hospital Research Institute

Publications and helpful links

General Publications

• Jenner AL, Cassidy T, Belaid K, Bourgeois-Daigneault MC, Craig M. In silico trials predict that combination strategies for enhancing vesicular stomatitis oncolytic virus are determined by tumor aggressivity. J Immunother Cancer. 2021 Feb;9(2):e001387. doi: 10.1136/jitc-2020-001387. Erratum In: J Immunother Cancer. 2021 Oct;9(10):
• Pol JG, Acuna SA, Yadollahi B, Tang N, Stephenson KB, Atherton MJ, Hanwell D, El-Warrak A, Goldstein A, Moloo B, Turner PV, Lopez R, LaFrance S, Evelegh C, Denisova G, Parsons R, Millar J, Stoll G, Martin CG, Pomoransky J, Breitbach CJ, Bramson JL, Bell JC, Wan Y, Stojdl DF, Lichty BD, McCart JA. Preclinical evaluation of a MAGE-A3 vaccination utilizing the oncolytic Maraba virus currently in first-in-human trials. Oncoimmunology. 2018 Sep 19;8(1):e1512329. doi: 10.1080/2162402X.2018.1512329. eCollection 2019.

Study record dates

Study Major Dates

• Study Start (Actual): January 22, 2015
• Primary Completion (Actual): September 19, 2019
• Study Completion (Estimated): December 31, 2024

Study Registration Dates

• First Submitted: October 31, 2014
• First Submitted That Met QC Criteria: November 6, 2014
• First Posted (Estimated): November 7, 2014

Study Record Updates

• Last Update Posted (Estimated): February 26, 2024
• Last Update Submitted That Met QC Criteria: February 23, 2024
• Last Verified: February 1, 2024

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Virus Diseases; Infections; DNA Virus Infections; Adenoviridae Infections
• Other Study ID Numbers: I214

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No