Mapping Human Muscle Satellite Cell Fate By Real-Time Imaging (S48)

Mapping Human Muscle Satellite Cell Fate By Real-Time

Mapping Human Muscle Satellite Cell Fate By Real-Time Imaging

Study Overview

• Status: Completed
• Conditions: Flaccid Muscle Tone
• Intervention / Treatment: Procedure: muscle biopsy

Detailed Description

The aim of this study is to obtain human muscle samples for cell culturing to analyze muscle cell development in real-time to uncover factors that influence growth.

• Study Type: Interventional
• Enrollment (Actual): 7
• Phase: Not Applicable

Contacts and Locations

Study Locations

• United States
  • Indiana
    • West Lafayette, Indiana, United States, 47907
      • Purdue University - Stone Hall 700 W State St

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 20 years to 29 years (ADULT)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Male

Description

-Inclusion Criteria:

• men 20-29 yrs
• BMI between 20 and 34.9
• Fasting Glucose <110 mg/dL
• Blood Pressure <160/100mm Hg
• Non-Diabetic
• LDL-Cholesterol <160mg/dL
• Triacylglycerol <400 mg/dL
• Makers of kidney, liver, and heart functions within 10% of clinical normalcy
• <1.5hr /wk of habitual aerobic exercise training and no resistance exercise training in the past 6 months

Exclusion Criteria:

• men <20or>29 yrs

  • BMI between <20 and >34.9
  • Fasting Glucose >110 mg/dL
  • Blood Pressure >160/100mm Hg
  • Non-Diabetic
  • LDL-Cholesterol >160mg/dL
  • Triacylglycerol >400 mg/dL

  • Pre-existing kidney or liver conditions

    -->1.5hr /wk of habitual aerobic exercise training and no resistance exercise training in the past 6 months

  • Currently taking prescribed blood thinners

Study Plan

How is the study designed?

Design Details

• Primary Purpose: OTHER
• Allocation: NA
• Interventional Model: SINGLE_GROUP
• Masking: NONE

Number of Arms

1

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
EXPERIMENTAL: muscle biopsy; a muscle sample will be taken from the mid-thigh (Vastus Lateralis) and analyzed	Procedure: muscle biopsy; a muscle sample will be taken from the mid-thigh (Vastus Lateralis) and analyzed

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Mapping Human Muscle Satellite Cell Fate By Real-Time Imaging	this study is to obtain human muscle samples for cell culturing which will be exposed to a novel real-time imaging technique in order to elucidate factors that influence muscle satellite cell growth.	<10 hrs

Collaborators and Investigators

• Sponsor: Purdue University

Study record dates

Study Major Dates

• Study Start: February 1, 2016
• Primary Completion (ACTUAL): May 1, 2016
• Study Completion (ACTUAL): June 1, 2016

Study Registration Dates

• First Submitted: February 10, 2016
• First Submitted That Met QC Criteria: February 12, 2016
• First Posted (ESTIMATE): February 18, 2016

Study Record Updates

• Last Update Posted (ACTUAL): June 20, 2017
• Last Update Submitted That Met QC Criteria: June 19, 2017
• Last Verified: June 1, 2017

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Nervous System Diseases; Neurologic Manifestations; Neuromuscular Manifestations; Muscle Hypotonia
• Other Study ID Numbers: 1512016862

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO