Severe Legionellosis Cases Admitted to Grenoble University Hospital, 2006-2011.

Molecular Epidemiology of Legionellosis in Grenoble Region

Sponsors

Lead Sponsor: University Hospital, Grenoble

Source University Hospital, Grenoble
Brief Summary

The study aimed at further describing the epidemiological, clinical, diagnostic and prognostic features in legionellosis cases admitted to Grenoble University Hospital during the 2006-2011period. The investigators also tested lower respiratory samples collected from these patients during their routine medical care, using a number of molecular tools allowing determination of the involved Legionella species, the bacterial load, and the presence of antibiotic resistant mutants. Our primary goal was to define biological markers that could predict severity and outcome of infection in legionellosis cases requiring hospitalization.

Detailed Description

Patients admitted to Grenoble University Hospital between 2006 and 2011 for severe legionellosis were retrospectively included in the study. Clinical, epidemiological, diagnostic and prognostic data of these patients were recorded retrospectively. Lower respiratory samples collected from these patients on a routine basis for microbiological analysis, especially Legionella culture, were tested using traditional microbiological methods. The remaining part of these samples was stored at -80°C fur further molecular analyses. The molecular tools developed by our bacteriology laboratory included the following: a real-time polymerase chain reaction (qPCR) test allowing detection and differentiation of L. pneumophila and other Legionella species; a qPCR test allowing determination of the Legionella DNA load in lower respiratory samples; a qPCR allowing detection of gyrA (the gene encoding subunit A of DNA gyrase) mutations that the investigators previously determined to be correlated with fluoroquinolones resistance in L. pneumophila; a high-throughput sequencing approach allowing confirmation of gyrA mutations and determination of the percentage of mutated alleles. The microbiological data, especially those obtained with the developped molecular tools, were tentatively correlated with the severity and outcome of infection in our patients' cohort.

Overall Status Completed
Start Date 2009-09-01
Completion Date 2014-12-01
Primary Completion Date 2011-12-01
Study Type Observational
Primary Outcome
Measure Time Frame
Legionella DNA load in lower respiratory tract samples through study completion, an average of 1 year
Secondary Outcome
Measure Time Frame
Fluoroquinolones resistant gyrA mutants in lower respiratory tract samples through study completion, an average of 1 year
Enrollment 82
Condition
Intervention

Intervention Type: Biological

Intervention Name: Real-time PCR testing of lower respiratory tract samples

Description: qPCR and high-throughput DNA sequencing testing of lower respiratory tract samples collected routinely in patients with severe legionellosis

Arm Group Label: Severe legionellosis cases

Eligibility

Sampling Method:

Non-Probability Sample

Criteria:

Inclusion Criteria: - age higher than 18 years - proven legionellosis case (positive culture and/or PCR and/or urinary antigen test) - severe case requiring hospitalization Exclusion Criteria: - age under 18 years - pregnant women

Gender:

All

Minimum Age:

18 Years

Maximum Age:

99 Years

Healthy Volunteers:

No

Overall Official
Last Name Role Affiliation
Hélène Sabbah-Guillaume, Director Study Director Direction of Research and Medical Innovation (DRCI)
Verification Date

2016-08-01

Responsible Party

Type: Principal Investigator

Investigator Affiliation: University Hospital, Grenoble

Investigator Full Name: AdministrateurDRC

Investigator Title: Pr. Max Maurin

Keywords
Has Expanded Access No
Condition Browse
Arm Group

Label: Severe legionellosis cases

Description: Patients admitted to Grenoble University Hospital for severe legionellosis between 2006 and 2011.

Acronym LEGIOGRE
Patient Data No
Study Design Info

Observational Model: Cohort

Time Perspective: Retrospective

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