Adiponectin, ICAM-1, VCAM-1 Levels and Metabolic Syndrome in Obese Adolescents (ICAM; VCAM)

Adipose tissue secreting a number of adipokines which regulate insulin sensitivity, energy metabolism and vascular homeostasis, so the dysfunction of adipose tissue is linked with the incidence of obesity accompanied with insulin resistance, hypertension and cardiovascular disease (1). Obesity is known to alters the expression of adipokines due to the adipose tissue hypertrophy (2), including adiponectin, in which able to exert a potent anti-inflammatory and vascular protective effect (2).

It has been proposed that adiponectin acts to prevent the vascular dysfunction due to obesity and diabetes by improves insulin sensitivity and metabolic profiles to reduce the risk factors for cardiovascular disease and protects the vasculature through its pleiotropic actions on endothelial cells, endothelial progenitor cells, smooth muscle cells and macrophages (1). The concentrations of adiponectin of 5 to 25 mg/mL had a significant inhibitory effect on the expression of monocyte adhesion and adhesion molecule induced by TNF-α in vitro. Atherosclerosis is an inflammatory disease in which adhesion molecules on arterial endothelial cells are responsible for the accumulation of monocytes/macrophages and T lymphocytes. While obesity is low-grade inflammation in which make a contribution on endothelial dysfunction by increasing the oxygen-derived free radicals (ROS) due to adipocyte hypertrophy, leads to an endoplasmic reticulum (ER) stress and mitochondrial dysfunction (3). Adiponectin is accumulated in the vasculature, and it reduced on obesity due to suppression by TNF-α and lead to adiponectin-deficiency which stimulate the significant increases of Vascular cell adhesion protein 1 (VCAM-1) and Intercellular Adhesion Molecule 1 (ICAM-1) or known as CD54 in aortic intima (4).

Here we investigate the level of adiponectin, ICAM-1, VCAM-1 with the incidence of MetS in obese adolescents.

Study Overview

• Status: Completed
• Conditions: Hyperglycemia; Hypertension; Obesity; Metabolic Syndrome; Hypertriglyceridemia; Abdominal Obesity; HDL Cholesterol, Low Serum
• Intervention / Treatment: Diagnostic test: Nur Aisiyah Widjaja

Detailed Description

A cross sectional study with healthy obese adolescents aged 13 until 18 years old were conducted during October 2019 to January 2020. Obesity was determined based on body mass index (BMI) for age based on gender > percentile 95th of CDC growth chart 2000.

Body weight was measured using Seca Robusta 813 digital scale in standing position, by stepping on the scale. While Body height was measured using Seca 206 Body Meter. Height was measured from the vertex of the head to the heel in standing position. Waist circumference and hip circumference were measured using Seca 201 measuring tape. Waist circumference was measured by wrapping the measuring tape around the subject's stomach, at the midpoint between the lowest rib and the endpoint of the iliac crest upon expiration, in line with the navel. Hip circumference was measured by asking the subjects to keep the feet together, then wrapping the measuring tape around the widest part of hips, at the point of the greatest gluteal protuberance. The subjects were measured using light cloth without food wear and other accessories such as belt, hat, or hair accessories, etc.

Blood pressure was measure using Omron Automatic Blood Pressure Monitor HEM-8712 (Omron Health Care Co., Ltd, Japan) by placing the cuff on the right arm, then pull and tighten it according to the size if the arm. After it was installed correctly (fasten and did not move), the power button on the digital tension tool was pressed, and then the microprocessor started to drive air pressure into the cuff, and then the value of blood pressures will appear in the manometer tube column.

Metabolic syndrome criteria were determined using The International Diabetic Federation criteria18:

1. For adolescent aged 10-16 years old: if there was a central obesity (waist circumference > 90th percentile based on WHO waist circumference table. For boys at the circumference of > 88 cm, and ≥ 85 cm for girls) accompanied with at least 2 other signs below:

   • Blood pressure > 90th percentile (systole ≥130/diastole ≥ 85 mmHg).
   • Hypertriglyceridemia, if the triglyceride levels > 110 mg/dl.
   • Low level HDL-c, if the HDL-c levels < 40 mg/dl, and < 50 mg/dl for girls.
   • Hyperglycaemia, if fasting blood glucose (FBG) levels was > 110 mg/dl.

2. For adolescent aged more than 16 years old: if there was a central obesity (waist circumference > 90th percentile based on WHO waist circumference table. For boys at the circumference of > 94 cm, and ≥ 80 cm for girls) accompanied with at least 2 other signs below:

   • Blood pressure > 90th percentile (systole ≥130/diastole ≥ 85 mmHg).
   • Hypertriglyceridemia, if the triglyceride levels ≥ 150 mg/dl.
   • Low level HDL-c, if the HDL-c levels <40 mg/dl for boys, and < 50 mg/dl for girls.
   • Hyperglycaemia, if fasting blood glucose (FBG) levels was ≥ 100 mg/dl19. Blood samples were withdrawn via vena cubitus as much as 5 ml by the trained analysts, and placed on vacutainer with EDTA. After that, the vacutainer was replaced in icebox to transport to the laboratory. Blood analysis include: lipid profile, fasting blood glucose, fasting insulin and adiponectin using the ELISA method in the laboratory. Intercellular Adhesion Molecule-1 (ICAM-1) was analyzed using Human intercellular adhesion molecule 1 Elisa kit (Bioassay Technology Laboratory). while sVCAM-1 was analysed using Human vascular cell adhesion molecule-1 Elisa kit (Bioassay Technology Laboratory).

Statistical analysis conducted were test of normality and homogeneity test, Anova/ Kruskal-Wallis, Independent sample T-test/Mann-Whitney U test, and Spearman correlation, and determined as significant if p value<0.05.

• Study Type: Observational
• Enrollment (Actual): 125

Contacts and Locations

Study Locations

• Indonesia
  • East Java
    • Surabaya, East Java, Indonesia, 60286
      • Institute of Tropical Disease (ITD) Airlangga University, Campus C

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 13 years to 18 years (Child, Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

• Sampling Method: Non-Probability Sample

Study Population

The subjects were obese adolescents who were studied at Junior and Senior High School in Surabaya and Sidoarjo, East Java, Indonesia

Description

Inclusion Criteria:

• Obesity
• Healthy (no medication treatment)

Exclusion Criteria:

• Taking corticosteroids or dyslipidemia medication
• Smoking or drinking (consuming alcohol)
• Taking antibiotics or hormonal therapy
• Autoimmune disease
• Endocrinology disorders

Study Plan

How is the study designed?

Design Details

• Observational Models: Other
• Time Perspectives: Cross-Sectional

Number of groups / cohorts

1

Cohorts and Interventions

Group / Cohort	Intervention / Treatment
Metablic syndrome vs. non-metabolic syndrome; Obese adolescents were grouped on MetS vs. non-MetS based on The International Diabetic Federation criteria	Diagnostic test: Nur Aisiyah Widjaja; Blood analysis for ICAM-1, VCAM-1, blood fasting insulin, lipid profile, fasting blood glucose and adiponectin investigation; Other Names: Suhasta Nova; Leonardo Alexander Caesar

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
ICAM-1 levels	Intercellular Adhesion Molecule 1 also known as CD54 was measured Human intercellular adhesion molecule 1 Elisa kit (Bioassay Technology Laboratory), stated as pg/ml (mean +/- SD)	4 months
VCAM-1	Vascular cell adhesion molecule 1 was measured using Human vascular cell adhesion molecule-1 Elisa kit (Bioassay Technology Laboratory), stated as pg/ml (mean +/- SD)	4 months
Adiponectin	For Adiponectin, ICAM-1 and VCAM-1 analysis, the blood that has been taken was not immediately analysed. The blood was centrifuged to remove the serum, and then stored at -70°C until the analysis was performed in the laboratory. Adiponectin was analysed using using Human Adiponectin Elisa kit (Bioassay Technology Laboratory), stated as ng/ml (mean +/- SD)	4 months

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Physical Measurements: Body weight	body weight was measured using Seca Robusta 813 digital scale in standing position by stepping on the scale. The subjects were asked to use light clothes without footwear or accessories. Stated as kg (mean +/- SD)	4 months
Physical Measurements: Body height	Body height was measured using Seca 206 Body Meter. Height was measured from the vertex of the head to the heel in standing position without footwear or accessories such as hat	4 months

Other Outcome Measures

Outcome Measure	Measure Description	Time Frame
Physical Measurements: Waist Circumference	Waist circumference was measured by wrapping the measuring tape (Seca 201 measuring tape),around the subject's stomach, at the midpoint between the lowest rib and the endpoint of the iliac crest upon expiration, in line with the navel. Stated as cm (mean +/- SD)	4 months
Physical Measurements: Hip Circumference	Hip circumference was measured by asking the subjects to keep the feet together, then wrapping the measuring tape (Seca 201 measuring tape) around the widest part of hips, at the point of the greatest gluteal protuberance, stated as cm (mean +/- SD)	4 months
Blood Pressure: Systole and diastole	Blood pressure was measured using Omron Automatic Blood Pressure Monitor HEM-8712, Omron Health Care Co., Ltd, Japan. The cuff was placed on the right arm, then pull and tighten it according to the size if the arm. After it was installed correctly (fasten and did not move), the power button on the digital tension tool was pressed, and then the microprocessor started to drive air pressure into the cuff, and then the value of blood pressures will appear in the manometer tube column. Stated as mmHg (mean +/- SD)	4 months
Blood biochemistry: HDL-c levels	The HDL-c levels were analysed by a designated medical laboratory after withdrawn, stated as mg/dl (mean +/- SD)	4 months
Blood biochemistry: Triglyceride levels	The triglyceride levels were analysed by a designated medical laboratory after withdrawn, stated as mg/dl (mean +/- SD)	4 months
Blood biochemistry: Fasting blood glucose levels	Fasting blood glucose levels were analysed by a designated medical laboratory after withdrawn, stated as mg/dl (mean +/- SD)	4 months
Blood biochemistry: Fasting insulin levels	Fasting insulin levels were analysed by a designated medical laboratory after withdrawn, stated as mg/dl (mean +/- SD)	4 months

Collaborators and Investigators

• Sponsor: Universitas Airlangga
• Investigators: Principal Investigator: Andy Darma, Paediatrician, Child Health Departement Airlangga University

Study record dates

Study Major Dates

• Study Start (Actual): October 1, 2019
• Primary Completion (Actual): January 30, 2020
• Study Completion (Actual): June 30, 2020

Study Registration Dates

• First Submitted: January 1, 2023
• First Submitted That Met QC Criteria: January 6, 2023
• First Posted (Estimate): January 13, 2023

Study Record Updates

• Last Update Posted (Estimate): January 13, 2023
• Last Update Submitted That Met QC Criteria: January 6, 2023
• Last Verified: January 1, 2023

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Pathologic Processes; Glucose Metabolism Disorders; Metabolic Diseases; Disease; Overnutrition; Nutrition Disorders; Overweight; Body Weight; Genetic Diseases, Inborn; Insulin Resistance; Hyperinsulinism; Metabolism, Inborn Errors; Lipid Metabolism Disorders; Hyperlipidemias; Dyslipidemias; Lipid Metabolism, Inborn Errors; Hypolipoproteinemias; Hyperglycemia; Syndrome; Obesity; Metabolic Syndrome; Hypertriglyceridemia; Obesity, Abdominal; Hypoalphalipoproteinemias
• Other Study ID Numbers: Secondary ID

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: No

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No