Acute Effects of Isoenergetic HIIE vs. MICE on Key Parameters of Fat Mass Loss in Young Females With Overweight or Obesity (COMEFIT)
August 27, 2026 updated by: Laboratoire des Adaptations Métaboliques à l'Exercice en conditions Physiologiques et Pathologiques
Acute Physiological Responses to Isoenergetic High-Intensity Interval vs. Moderate-Intensity Continuous Exercise in Overweight or Obese Young Females: Focus on Catecholamines, Oxygen Consumption, Lipid Utilization, and Appetite Regulation
Overweight and obesity, particularly increased total and/or abdominal fat mass (FM), are strongly linked to the development of cardiovascular diseases. Managing obesity effectively requires lifestyle and dietary modifications, including increased physical activity. Among the various exercise strategies, Moderate-Intensity Continuous Training (MICT) and High-Intensity Interval Training (HIIT) are widely recommended for reducing FM. Compared to MICT, HIIT is a time-efficient approach, often perceived as more enjoyable, and has been shown to promote greater total and abdominal fat loss in a shorter period. However, the precise mechanisms underlying this enhanced fat loss remain unclear.
This study aims to compare the acute effects of two isoenergetic exercise sessions - MICE (Moderate-Intensity Continuous Exercise) and HIIE (High-Intensity Interval Exercise) - on key physiological responses involved in fat mass loss. Specifically, we will assess plasma catecholamine levels during exercise and recovery, as well as oxygen consumption, substrate oxidation, and appetite over the 2-hour post-exercise period in young females with overweight or obesity. By elucidating the role of catecholaminergic stimulation as a potential regulator of fat mass loss, this research could provide valuable insights for optimizing exercise strategies in weight management and metabolic health.
Study Overview
Status
Recruiting
Intervention / Treatment
Study Type
Interventional
Enrollment (Estimated)
15
Phase
- Not Applicable
Contacts and Locations
This section provides the contact details for those conducting the study, and information on where this study is being conducted.
Study Locations
-
-
São Paulo
-
São Paulo, São Paulo, Brazil, 475
- Recruiting
- Laboratório de Fisiologia e Metabolismo Aplicados à Educação Física
-
Contact:
- JULIANA MONIQUE LINO APARECIDO, PhD
- Phone Number: (+55) 1196997-5162
- Email: juliana.monique@unicid.edu.br
-
Contact:
- Nathalie Boisseau, Professor
- Phone Number: (+33) 0684331693
- Email: nathalie.boisseau@uca.fr
-
-
Participation Criteria
Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.
Eligibility Criteria
Ages Eligible for Study
- Adult
Accepts Healthy Volunteers
Yes
Description
Inclusion Criteria:
- women with overwieght or obesity (BMI: 25-35 kg/m²)
Exclusion Criteria:
- contraceptive use
- menopause
- irregular menstrual cycle
- medical contraindications to intense physical activity,
- painful joints,
- taking ß-blocker
Study Plan
This section provides details of the study plan, including how the study is designed and what the study is measuring.
How is the study designed?
Design Details
- Primary Purpose: Prevention
- Allocation: Non-Randomized
- Interventional Model: Crossover Assignment
- Masking: Single
Arms and Interventions
Participant Group / Arm |
Intervention / Treatment |
|---|---|
|
Experimental: High Intensity Interval Exercise
High Intensity Interval Exercise: 5 minutes of WarmUp + 10 x (1 minute at 85% HRmax followed by 1 minute of recovery at a power equivalent to 40% of HRmax)
|
Two Exercise Modalities: HIIE and MICE
|
|
Experimental: Moderate Intensity Continuous Exercise
Moderate Intensity Continuous Exercise: 5 minutes of WarmUp + 35 minutes at 55% HRmax
|
Two Exercise Modalities: HIIE and MICE
|
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
|---|---|---|
|
Oxygen consumption during exercise and the recovery period
Time Frame: Measurement at rest for 20 minutes, then during warm-up for 10 minutes, then during exercise for 20 minutes and finally during 2 hours after the end of the exercise.
|
Determination of oxygen consumption during exercise and 2 hours after the exercise session (HIIE and MICE).
Determination from Metamax (3D Cortex).
|
Measurement at rest for 20 minutes, then during warm-up for 10 minutes, then during exercise for 20 minutes and finally during 2 hours after the end of the exercise.
|
Secondary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
|---|---|---|
|
Lipid before and after the exercise.
Time Frame: Measurement at rest for 20 minutes and then during 2 hours after the end of the exercise.
|
Determination of lipid oxidation before and after the exercise.
Lipid oxidation is measured from oxygen and carbon dioxide consumption (Metamax 3D Cortex).
|
Measurement at rest for 20 minutes and then during 2 hours after the end of the exercise.
|
|
Carbohydrate before and after the exercise.
Time Frame: Measurement at rest for 20 minutes and then during 2 hours after the end of the exercise.
|
Determination of carbohydrate oxidation before and after the exercise.
Carbohydrate oxidation is measured from oxygen and carbon dioxide consumption (Metamax 3D Cortex).
|
Measurement at rest for 20 minutes and then during 2 hours after the end of the exercise.
|
|
Catecholamines
Time Frame: 5 collections: before exercise started, at the end of exercise (exercise duration is 20-35 minutes), at 30 minutes, 60 minutes and 120 minutes after the end of exercise
|
Determination of cathecolamines concentration in blood during exercise and 2 hours after the end of the exercise.
|
5 collections: before exercise started, at the end of exercise (exercise duration is 20-35 minutes), at 30 minutes, 60 minutes and 120 minutes after the end of exercise
|
|
Energy intakes 24 hours after the exercise
Time Frame: 24 hours post exercise
|
Evaluation of energy intakes after the exercise session (HIIE vs MICE).
Exercise session finish at 11:30 a.m.
After that, at home, participants have to complete by themselves a food-record on which they will describe all their meal during 24 hours after the end of the exercise session.
It includes their lunch, dinner and their breakfast the next morning.
|
24 hours post exercise
|
Collaborators and Investigators
This is where you will find people and organizations involved with this study.
Publications and helpful links
The person responsible for entering information about the study voluntarily provides these publications. These may be about anything related to the study.
General Publications
- Dupuit M, Rance M, Morel C, Bouillon P, Pereira B, Bonnet A, Maillard F, Duclos M, Boisseau N. Moderate-Intensity Continuous Training or High-Intensity Interval Training with or without Resistance Training for Altering Body Composition in Postmenopausal Women. Med Sci Sports Exerc. 2020 Mar;52(3):736-745. doi: 10.1249/MSS.0000000000002162.
- Dupuit M, Boscaro A, Bonnet A, Bouillon P, Bruno P, Morel C, Rance M, Boisseau N. Acute metabolic responses after continuous or interval exercise in post-menopausal women with overweight or obesity. Scand J Med Sci Sports. 2020 Dec;30(12):2352-2363. doi: 10.1111/sms.13814. Epub 2020 Sep 18.
- Cunha FA, Midgley AW, McNaughton LR, Farinatti PT. Effect of continuous and intermittent bouts of isocaloric cycling and running exercise on excess postexercise oxygen consumption. J Sci Med Sport. 2016 Feb;19(2):187-92. doi: 10.1016/j.jsams.2015.02.004. Epub 2015 Feb 23.
Study record dates
These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.
Study Major Dates
Study Start (Actual)
February 1, 2025
Primary Completion (Estimated)
December 30, 2026
Study Completion (Estimated)
December 30, 2026
Study Registration Dates
First Submitted
February 21, 2025
First Submitted That Met QC Criteria
March 4, 2025
First Posted (Actual)
March 11, 2025
Study Record Updates
Last Update Posted (Actual)
August 31, 2026
Last Update Submitted That Met QC Criteria
August 27, 2026
Last Verified
August 1, 2026
More Information
Terms related to this study
Keywords
Additional Relevant MeSH Terms
Other Study ID Numbers
- CAAE: 79561024.4.0000.9487
Plan for Individual participant data (IPD)
Plan to Share Individual Participant Data (IPD)?
NO
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
No
Studies a U.S. FDA-regulated device product
No
product manufactured in and exported from the U.S.
No
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.