Eccentric Exercise, Muscle Damage, and Oxygen Uptake Across Menstrual Cycle Phases (EEMDO-MC)

May 13, 2026 updated by: Vassilis Paschalis, National and Kapodistrian University of Athens

The Effect of Eccentric Exercise on Biomarkers of Exercise Induced Muscle Damage and Oxygen Uptake: Differences Between Phases of the Menstrual Cycle

The existing literature examining the effect of female sex hormones across the phases of the menstrual cycle on muscle microdamage and oxygen uptake is limited. The protective role of estrogens against muscle microdamage is supported by findings from some studies; however, it is questioned by others. The aim of the present study was to investigate the effect of eccentric exercise on biomarkers of muscle microdamage and on oxygen uptake during two phases of the menstrual cycle in healthy premenopausal women.

Study Overview

Status

Completed

Conditions

Intervention / Treatment

Study Type

Interventional

Enrollment (Actual)

12

Phase

  • Not Applicable

Contacts and Locations

This section provides the contact details for those conducting the study, and information on where this study is being conducted.

Study Locations

  • Greece
    • Attica
      • Dafni, Attica, Greece, 17234
        • Department of Physical Educaiton and Sport Science

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

Eligibility Criteria

Ages Eligible for Study

  • Adult

Accepts Healthy Volunteers

Yes

Description

Inclusion Criteria:

  • No known medical history
  • Not receiving any medication or dietary supplementation.
  • Stable menstrual cycle

Exclusion Criteria:

  • Pathological conditions the last month before enrolment in the study
  • Low limb injury the past 4 months
  • Unstable menstrual cycle

Study Plan

This section provides details of the study plan, including how the study is designed and what the study is measuring.

How is the study designed?

Design Details

  • Primary Purpose: Basic Science
  • Allocation: N/A
  • Interventional Model: Single Group Assignment
  • Masking: None (Open Label)

Arms and Interventions

Participant Group / Arm
Intervention / Treatment
Experimental: Eccentric exercise
A group of female subjects performed an exercise with one leg and an exercise with the other leg, separated by 2 weeks. Before and 2 days after the exercise sessions, performance was assessed.
Other: Isokinetic eccentric exercise using knee extensors of the right lower limb
Isokinetic eccentric exercise consisted of 10 min at 40% of maximal voluntary contractions, followed by 2 min maximal intensity muscle effort
Other: Isokinetic eccentric exercise using knee extensors of the left lower limb
Isokinetic eccentric exercise consisted of 10 min at 40% of maximal voluntary contractions, followed by 2 min maximal intensity muscle effort

What is the study measuring?

Primary Outcome Measures

Outcome Measure
Measure Description
Time Frame
Maximal isometric torque
Time Frame: From enrollment to the end of the intervention at 2 weeks
maximal performance during 5 sec isometric knee extensors contraction, assessed pre and 48 h post eccentric exercise
From enrollment to the end of the intervention at 2 weeks
Delayed onset muscle soreness
Time Frame: From enrollment to the end of intervention at 2 weeks
Palpation of the muscle belly, performed pre and 48h post eccentric exercise
From enrollment to the end of intervention at 2 weeks
Oxygen consumption
Time Frame: From enrollment to the end of the intervention at 2 weeks
During the isokinetic eccentric exercise, participants were attached to a gas analyser for continuously measuring oxygen consumption.
From enrollment to the end of the intervention at 2 weeks
Duration of the isokinetic exercise
Time Frame: From enrollment to the end of intervention at 2 weeks
The total duration of exercise performed on the isokinetic dynamometer was recorded
From enrollment to the end of intervention at 2 weeks

Collaborators and Investigators

This is where you will find people and organizations involved with this study.

Sponsor

National and Kapodistrian University of Athens

Investigators

  • Principal Investigator: Vassilis Paschalis, PhD, National and Kapodistrian University of Athens

Study record dates

These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.

Study Major Dates

Study Start (Actual)

May 15, 2025

Primary Completion (Actual)

August 5, 2025

Study Completion (Actual)

January 22, 2026

Study Registration Dates

First Submitted

May 6, 2026

First Submitted That Met QC Criteria

May 6, 2026

First Posted (Actual)

May 12, 2026

Study Record Updates

Last Update Posted (Actual)

May 15, 2026

Last Update Submitted That Met QC Criteria

May 13, 2026

Last Verified

May 1, 2026

More Information

Terms related to this study

Keywords

Other Study ID Numbers

  • 1723/19-12-2024

Drug and device information, study documents

Studies a U.S. FDA-regulated drug product

No

Studies a U.S. FDA-regulated device product

No

This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.

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