Acute Effects of Post-Exercise Hyperoxia on Recovery in Cycling

This study examines whether exposition to hyperbaric oxygen after a road-race simulation can help competitive cyclists recover and perform better the following day.

Hyperbaric oxygen, which involves breathing oxygen inside a pressurized chamber, is used as a recovery method in elite and professional sport. Its effectiveness, however, remains controversial: despite this widespread use, there is a lack of solid scientific evidence that a single HBO session after strenuous endurance exercise actually improves recovery, or that clarifies how the amount of oxygen exposure influences any benefit.

The study includes healthy male road cyclists between 18 and 40 years of age who compete at the national level in Belgium. After completing a fatiguing cycling session, each participant is randomly assigned to one of four groups receiving different levels of oxygen exposure during recovery.

Two groups breathe oxygen under increased pressure inside a chamber at either 2.5 or 1.4 atmospheres absolute. A third group breathes oxygen at normal pressure. The fourth group receives a sham condition that reproduces the treatment setting without active oxygen exposure.

The study is double-blind, meaning that neither the participants nor the researchers assessing the outcomes know which condition each participant receives.

The main goal is to determine whether a single session of post-exercise HBO improves next-day endurance performance, and whether higher oxygen exposure produces greater effects.

The researchers also collect blood samples and physiological measurements to better understand how the body recovers.

Study Overview

• Status: Recruiting
• Conditions: Oxidative Stress; Athletic Performance; Muscle Fatigue; Physical Endurance; Hyperbaric Oxygenation
• Intervention / Treatment: Other: Post-exercise Recovery

• Study Type: Interventional
• Enrollment (Estimated): 48
• Phase: Not Applicable

Contacts and Locations

• Study Contact: Name: Théo A MAHY, MSc; Phone Number: +32 10 474 489; Email: theo.mahy@uclouvain.be

Study Locations

• Belgium
  • Brabant-Wallon
    • Louvain-la-Neuve, Brabant-Wallon, Belgium, 1348
      • Recruiting
      • Institute of Neuroscience, Université catholique de Louvain

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Adult
• Accepts Healthy Volunteers: Yes

Description

Inclusion Criteria:

• Road cyclists competing in Elite 2 (Elite without professional contract) or Under-23 (U23) categories or equivalent [Participation in national-level Belgian competitions within the previous 12 months. No specific ranking, finishing position, or performance threshold is required.]
• ≥ 2 consecutive years of cycling experience at regional or national level, with structured and periodized training
• Cycling-specific training volume ≥ 8 h a week over the preceding 10 weeks
• Stable training load in the weeks preceding inclusion, with no major changes due to injury, illness, or abnormal overload
• Measured VO₂max ≥ 60 ml/kg/min

Exclusion Criteria:

• Presence of significant cardiovascular, pulmonary, neurological, ENT, or metabolic disease, including but not limited to: Uncontrolled arterial hypertension (systolic blood pressure ≥ 180 mmHg and/or diastolic blood pressure ≥ 110 mmHg) ; Unstable heart disease or decompensated cardiomyopathy
• Contraindications to hyperbaric exposure, including: Untreated pneumothorax, History of spontaneous pneumothorax without medical clearance ; Pulmonary conditions associated with air trapping (e.g. emphysema or bullous lung disease), or abnormal thoracic imaging when indicated ; Recent thoracic surgery
• Neurological contraindications, including epilepsy, history of oxygen-induced seizures, or recent seizure activity, and any association with cannabis use
• Ear, sinus, or Eustachian tube disorders preventing adequate pressure equalization, including active infection or history of severe barotrauma
• Severe claustrophobia incompatible with chamber exposure

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: Double

Number of Arms

4

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: HBO 2.5 ATA; PiO₂ = 2.5 (100 % O₂ x 2.5 ATA)	Other: Post-exercise Recovery; 1h intervention after a fatiguing exercise
Experimental: HBO 1.4 ATA; PiO₂ = 1.4 (100 % O₂ x 1.4 ATA)	Other: Post-exercise Recovery; 1h intervention after a fatiguing exercise
Experimental: NBO 1.0 ATA; PiO₂ = 1.0 (100 % O₂ x 1.0 ATA)	Other: Post-exercise Recovery; 1h intervention after a fatiguing exercise
Sham Comparator: SHAM; PiO₂ = 0.21 (21 % O₂ x 1.0 ATA)	Other: Post-exercise Recovery; 1h intervention after a fatiguing exercise

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Next-day endurance performance : Percent decrement in time to exhaustion from baseline	Endurance performance measured by a cycling time-to-exhaustion test at 105% of the lactate threshold power, expressed as the percentage decrement in time to exhaustion relative to the baseline test. Performance is compared SHAM to the other oxygen-exposure groups.	Throughout the entire study, approximately during 18 months

Collaborators and Investigators

• Sponsor: Université Catholique de Louvain
• Collaborators: Murdoch University

Study record dates

Study Major Dates

• Study Start (Estimated): July 1, 2026
• Primary Completion (Estimated): December 31, 2027
• Study Completion (Estimated): December 31, 2027

Study Registration Dates

• First Submitted: June 15, 2026
• First Submitted That Met QC Criteria: June 15, 2026
• First Posted (Actual): June 22, 2026

Study Record Updates

• Last Update Posted (Actual): June 22, 2026
• Last Update Submitted That Met QC Criteria: June 15, 2026
• Last Verified: June 1, 2026

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena; Physiological Phenomena; Biological Phenomena; Exercise; Adaptation, Physiological; Adaptation, Biological; Post-Exercise Recovery
• Other Study ID Numbers: HBO-RISE

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No