Evaluation of the Effectiveness of Oral Ozonized Oil Supplementation in Elite Athletes

August 31, 2026 updated by: Izzotti, Alberto, Universita degli Studi di Genova

Evaluation of the Effects of Ozonated Oil Administration on Athletic Performance and Injury Prevention in Elite Athletes.

Ozonized oil has anti-inflammatory and antioxidant properties, but its effects on athletic performance remain unclear. This study evaluated whether daily ozonized oil supplementation, combined with training, influences aerobic capacity, anaerobic power, maximal strength, and muscular endurance in elite rugby players over six months

Study Overview

Status

Completed

Conditions

Intervention / Treatment

Study Type

Interventional

Enrollment (Actual)

33

Phase

  • Not Applicable

Contacts and Locations

This section provides the contact details for those conducting the study, and information on where this study is being conducted.

Study Locations

  • Italy
    • AQ
      • Avezzano, AQ, Italy, 67051
        • Stadio del Rugby
    • Genova
      • Genova, Genova, Italy, 16132
        • University of Genova

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

Eligibility Criteria

Ages Eligible for Study

  • Adult
  • Older Adult

Accepts Healthy Volunteers

Yes

Description

Inclusion Criteria:

  • Inclusion criteria were age ≥18

Exclusion Criteria:

  • NA

Study Plan

This section provides details of the study plan, including how the study is designed and what the study is measuring.

How is the study designed?

Design Details

  • Primary Purpose: Supportive Care
  • Allocation: Randomized
  • Interventional Model: Single Group Assignment
  • Masking: Single

Arms and Interventions

Participant Group / Arm
Intervention / Treatment
Experimental: Intervention group (IG): received daily ozonized oil supplementation

The IG received the supplement of ozonized oil capsules (O3ZONE Sport ®). Dosage was standardized and adjusted according to body:

  • ≥100 kg: 6 capsules/day (3 morning, 3 evening)
  • <100 kg: 4 capsules/day (2 morning, 2 evening) Adherence to the protocol was monitored weekly through self-report and supervision by team medical staff.

Both groups followed identical training protocols; the IG additionally received daily ozonized oil. Performance was assessed at baseline, 3 months, and 6 months using the Bronco test (field endurance), Bruce and Léger tests (estimated VO2max), the Running-Based Anaerobic Sprint Test (RAST), and tests of quadriceps and bench-press 1-repetition maximum (1RM) and endurance at 50% 1RM. Outcomes were expressed as percentage change from baseline
Dietary supplement: Ozonized Oil
, the present study aimed to investigate the potential effects of daily oral supplementation with ozonized oil (O3ZONE Sport®) (produced for O3 ZONE LIMITED, Acquaviva, RSM) on physical performance outcomes - aerobic capacity, maximal strength, muscular endurance, and functional fitness - in elite rugby players over a six-month training period.
No Intervention: • Control group (CG): n = 15, which continued standard training without supplementation

What is the study measuring?

Primary Outcome Measures

Outcome Measure
Measure Description
Time Frame
The primary objective was to investigate the effects of ozonated oil administration on athletic performance and injury prevention in a group of 21 rugby athletes, divided into two groups: Experimental group (n=13) vs Control group (n=8)
Time Frame: 1 year
Bruce test: which measures a subject's maximal oxygen consumption (VO2max). VO2max represents the maximum aerobic power, that is, the maximum amount of energy that can be synthesized by the aerobic mechanism in a unit of time.
1 year

Secondary Outcome Measures

Outcome Measure
Measure Description
Time Frame
The primary objective was to investigate the effects of ozonated oil administration on athletic performance and injury prevention in a group of 21 rugby athletes, divided into two groups: Experimental group (n=13) vs Control group (n=8)
Time Frame: 1 year
Bronco test: is a 1,200 meter shuttle run designed to measure maximum aerobic speed (MAS) and endurance, primarily used in rugby. The test requires athletes to complete five sets of 20 meter, 40 meter, and 60meter shuttle runs, totalling 1,200 meters as fast as possible
1 year
The primary objective was to investigate the effects of ozonated oil administration on athletic performance and injury prevention in a group of 21 rugby athletes, divided into two groups: Experimental group (n=13) vs Control group (n=8)
Time Frame: 1 year
Leger Test:or multi-stage fitness test, requires running back and forth between two markers placed exactly 20 meters (65.6 feet) apart. The pace is set by audio beeps, with speeds increasing every minute (level), typically starting at 8.5 km/h and increasing by 0.5 km/h each level
1 year

Collaborators and Investigators

This is where you will find people and organizations involved with this study.

Sponsor

Universita degli Studi di Genova

Study record dates

These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.

Study Major Dates

Study Start (Actual)

September 9, 2024

Primary Completion (Actual)

April 17, 2025

Study Completion (Actual)

April 18, 2025

Study Registration Dates

First Submitted

April 16, 2026

First Submitted That Met QC Criteria

August 31, 2026

First Posted (Actual)

September 3, 2026

Study Record Updates

Last Update Posted (Actual)

September 3, 2026

Last Update Submitted That Met QC Criteria

August 31, 2026

Last Verified

April 1, 2026

More Information

Terms related to this study

Other Study ID Numbers

  • C1LN001
  • 100008-2024-AIFFRA (Other Identifier: univeristy of Genova, university research funds)

Plan for Individual participant data (IPD)

Plan to Share Individual Participant Data (IPD)?

UNDECIDED

Drug and device information, study documents

Studies a U.S. FDA-regulated drug product

No

Studies a U.S. FDA-regulated device product

No

This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.

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