Effects of Exercise and Short Term Rapid Weight Loss on Whole Blood Cells and Lymphocyte Subsets in Highly Trained National Level Wrestlers (EX-RWL-Lymphoc)
13. maj 2026 opdateret af: Ankara Yildirim Beyazıt University
Effects of Exercise and Short-Term Rapid Weight Loss on Whole Blood Cells and Lymphocyte Subsets in Highly Trained National-Level Wrestlers: A Two-Phase Repeated-Measures Study
Wrestling is a weight-classified sport in which athletes frequently apply rapid weight loss (RWL) strategies prior to competition. While the performance-related consequences of RWL have been widely investigated, its combined effects with exercise on hematological parameters and immune cell profiles remain insufficiently understood. Therefore, this study aims to examine the effects of short-term weight loss and exercise on whole blood cell parameters and lymphocyte subsets in elite wrestlers.
Nine elite male wrestlers actively competing in the senior category (mean age: 22.90 ± 5.15 years; mean height: 1.75 m) voluntarily participated in this study. Athletes will achieve an average body mass reduction of 4.3% over a five-day period. Venous blood samples will be collected at 09:30 a.m. in a fasted state during two experimental phases: (1) Exercise-only phase, with blood sampling before and after exercise (BRWL1 and BRWL2); (2) Exercise plus weight loss phase, following the five-day weight loss period, with blood sampling before and after exercise (ARWL1 and ARWL2). Complete blood count parameters will be analyzed using an automated hematology analyzer, while lymphocyte subsets (CD3+, CD4+, CD8+, CD19+, CD16/56+) will be assessed by flow cytometry. Statistical analyses will be performed with a significance level set at p < 0.05.
Studieoversigt
Status
Afsluttet
Betingelser
Intervention / Behandling
Detaljeret beskrivelse
See brief summary for study protocol details
Undersøgelsestype
Interventionel
Tilmelding (Faktiske)
9
Fase
- Ikke anvendelig
Kontakter og lokationer
Dette afsnit indeholder kontaktoplysninger for dem, der udfører undersøgelsen, og oplysninger om, hvor denne undersøgelse udføres.
Studiesteder
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Ankara
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Ankara, Ankara, Tyrkiet (Türkiye), 06000
- Ankara Yildirim Beyazit University Sports Sciences Laboratory
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Deltagelseskriterier
Forskere leder efter personer, der passer til en bestemt beskrivelse, kaldet berettigelseskriterier. Nogle eksempler på disse kriterier er en persons generelle helbredstilstand eller tidligere behandlinger.
Berettigelseskriterier
Aldre berettiget til at studere
- Voksen
Tager imod sunde frivillige
Ja
Beskrivelse
Inclusion Criteria:
- Being between 18 and 35 years of age
- Being clinically healthy
- Actively competing in wrestling
- Having regular training experience
- No history of chronic disease
- No musculoskeletal injury that could affect performance
- Not using any medication or nutritional supplements that could influence hematological or biochemical parameters during the study period
- Willing to volunteer and provide written informed consent
Exclusion Criteria:
- Having any chronic disease (e.g., cardiovascular, metabolic, respiratory, or neurological disorders)
- Having a recent musculoskeletal injury that could affect exercise performance
- Using medications, ergogenic aids, or nutritional supplements that might influence hematological or biochemical parameters
- Having a history of smoking or alcohol abuse
- Failing to complete the testing procedures and training protocol required for the study
- Not providing written informed consent
- Withdrawing voluntarily during the study period
Studieplan
Dette afsnit indeholder detaljer om studieplanen, herunder hvordan undersøgelsen er designet, og hvad undersøgelsen måler.
Hvordan er undersøgelsen tilrettelagt?
Design detaljer
- Primært formål: Grundvidenskab
- Tildeling: Ikke-randomiseret
- Interventionel model: Sekventiel tildeling
- Maskning: Ingen (Åben etiket)
Våben og indgreb
Deltagergruppe / Arm |
Intervention / Behandling |
|---|---|
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Andet: Exercise
Participants performed a standardized wrestling exercise session on the first day of testing.
Venous blood samples were collected pre- and post-exercise (pre-exercise: BRWL1; post-exercise: BRWL2) to evaluate hematological and lymphocyte subset responses.
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Standardized wrestling exercise session; pre- and post-exercise venous blood collected
Andre navne:
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Andet: Exercise + Rapid Weight Loss
Participants underwent a 5-day rapid weight loss protocol prior to the exercise session, achieving an average body mass reduction of 4.3%.
On the fifth day, the same standardized exercise protocol as Group 1 was performed, and pre- and post-exercise blood samples were collected (pre-exercise: ARWL1; post-exercise: ARWL2) to evaluate hematological and lymphocyte subset responses.
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Standardized wrestling exercise session; pre- and post-exercise venous blood collected
Andre navne:
5-day rapid weight loss protocol achieving average 4.3% body mass reduction
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Hvad måler undersøgelsen?
Primære resultatmål
Resultatmål |
Foranstaltningsbeskrivelse |
Tidsramme |
|---|---|---|
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Change in Leukocyte Count
Tidsramme: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in total white blood cell (WBC) count measured from pre- to post-exercise
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Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in Lymphocyte Percentage
Tidsramme: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in percentage of lymphocytes relative to total WBC count, measured from pre- to post-exercise.
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Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in CD3+ T Cell Count
Tidsramme: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in absolute count of CD3+ T lymphocytes measured from pre- to post-exercise.
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Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in CD4+ T Cell Count
Tidsramme: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in absolute count of CD4+ helper T lymphocytes measured from pre- to post-exercise.
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Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in CD8+ T Cell Count
Tidsramme: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in absolute count of CD8+ cytotoxic T lymphocytes measured from pre- to post-exercise.
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Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in CD19+ B Cell Count
Tidsramme: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in absolute count of CD19+ B lymphocytes measured from pre- to post-exercise.
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Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in CD16/56+ NK Cell Count
Tidsramme: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in absolute count of CD16/56+ natural killer (NK) cells measured from pre- to post-exercise.
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Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Sekundære resultatmål
Resultatmål |
Foranstaltningsbeskrivelse |
Tidsramme |
|---|---|---|
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Change in Creatine Kinase and Lactic Acid Levels
Tidsramme: Pre-exercise and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Serum creatine kinase (CK) and lactic acid (LA) levels measured to assess anaerobic performance and muscle damage.
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Pre-exercise and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Samarbejdspartnere og efterforskere
Det er her, du vil finde personer og organisationer, der er involveret i denne undersøgelse.
Datoer for undersøgelser
Disse datoer sporer fremskridtene for indsendelser af undersøgelsesrekord og resumeresultater til ClinicalTrials.gov. Studieregistreringer og rapporterede resultater gennemgås af National Library of Medicine (NLM) for at sikre, at de opfylder specifikke kvalitetskontrolstandarder, før de offentliggøres på den offentlige hjemmeside.
Studer store datoer
Studiestart (Faktiske)
21. juni 2021
Primær færdiggørelse (Faktiske)
3. april 2022
Studieafslutning (Faktiske)
10. oktober 2022
Datoer for studieregistrering
Først indsendt
22. april 2026
Først indsendt, der opfyldte QC-kriterier
28. april 2026
Først opslået (Faktiske)
5. maj 2026
Opdateringer af undersøgelsesjournaler
Sidste opdatering sendt (Faktiske)
15. maj 2026
Sidste opdatering indsendt, der opfyldte kvalitetskontrolkriterier
13. maj 2026
Sidst verificeret
1. maj 2026
Mere information
Begreber relateret til denne undersøgelse
Nøgleord
Yderligere relevante MeSH-vilkår
Andre undersøgelses-id-numre
- 2280
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