Effects of Exercise and Short Term Rapid Weight Loss on Whole Blood Cells and Lymphocyte Subsets in Highly Trained National Level Wrestlers (EX-RWL-Lymphoc)
13. Mai 2026 aktualisiert von: Ankara Yildirim Beyazıt University
Effects of Exercise and Short-Term Rapid Weight Loss on Whole Blood Cells and Lymphocyte Subsets in Highly Trained National-Level Wrestlers: A Two-Phase Repeated-Measures Study
Wrestling is a weight-classified sport in which athletes frequently apply rapid weight loss (RWL) strategies prior to competition. While the performance-related consequences of RWL have been widely investigated, its combined effects with exercise on hematological parameters and immune cell profiles remain insufficiently understood. Therefore, this study aims to examine the effects of short-term weight loss and exercise on whole blood cell parameters and lymphocyte subsets in elite wrestlers.
Nine elite male wrestlers actively competing in the senior category (mean age: 22.90 ± 5.15 years; mean height: 1.75 m) voluntarily participated in this study. Athletes will achieve an average body mass reduction of 4.3% over a five-day period. Venous blood samples will be collected at 09:30 a.m. in a fasted state during two experimental phases: (1) Exercise-only phase, with blood sampling before and after exercise (BRWL1 and BRWL2); (2) Exercise plus weight loss phase, following the five-day weight loss period, with blood sampling before and after exercise (ARWL1 and ARWL2). Complete blood count parameters will be analyzed using an automated hematology analyzer, while lymphocyte subsets (CD3+, CD4+, CD8+, CD19+, CD16/56+) will be assessed by flow cytometry. Statistical analyses will be performed with a significance level set at p < 0.05.
Studienübersicht
Status
Abgeschlossen
Bedingungen
Intervention / Behandlung
Detaillierte Beschreibung
See brief summary for study protocol details
Studientyp
Interventionell
Einschreibung (Tatsächlich)
9
Phase
- Unzutreffend
Kontakte und Standorte
Dieser Abschnitt enthält die Kontaktdaten derjenigen, die die Studie durchführen, und Informationen darüber, wo diese Studie durchgeführt wird.
Studienorte
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Ankara
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Ankara, Ankara, Türkei (türkiye), 06000
- Ankara Yildirim Beyazit University Sports Sciences Laboratory
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Teilnahmekriterien
Forscher suchen nach Personen, die einer bestimmten Beschreibung entsprechen, die als Auswahlkriterien bezeichnet werden. Einige Beispiele für diese Kriterien sind der allgemeine Gesundheitszustand einer Person oder frühere Behandlungen.
Zulassungskriterien
Studienberechtigtes Alter
- Erwachsene
Akzeptiert gesunde Freiwillige
Ja
Beschreibung
Inclusion Criteria:
- Being between 18 and 35 years of age
- Being clinically healthy
- Actively competing in wrestling
- Having regular training experience
- No history of chronic disease
- No musculoskeletal injury that could affect performance
- Not using any medication or nutritional supplements that could influence hematological or biochemical parameters during the study period
- Willing to volunteer and provide written informed consent
Exclusion Criteria:
- Having any chronic disease (e.g., cardiovascular, metabolic, respiratory, or neurological disorders)
- Having a recent musculoskeletal injury that could affect exercise performance
- Using medications, ergogenic aids, or nutritional supplements that might influence hematological or biochemical parameters
- Having a history of smoking or alcohol abuse
- Failing to complete the testing procedures and training protocol required for the study
- Not providing written informed consent
- Withdrawing voluntarily during the study period
Studienplan
Dieser Abschnitt enthält Einzelheiten zum Studienplan, einschließlich des Studiendesigns und der Messung der Studieninhalte.
Wie ist die Studie aufgebaut?
Designdetails
- Hauptzweck: Grundlegende Wissenschaft
- Zuteilung: Nicht randomisiert
- Interventionsmodell: Sequenzielle Zuweisung
- Maskierung: Keine (Offenes Etikett)
Waffen und Interventionen
Teilnehmergruppe / Arm |
Intervention / Behandlung |
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Sonstiges: Exercise
Participants performed a standardized wrestling exercise session on the first day of testing.
Venous blood samples were collected pre- and post-exercise (pre-exercise: BRWL1; post-exercise: BRWL2) to evaluate hematological and lymphocyte subset responses.
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Standardized wrestling exercise session; pre- and post-exercise venous blood collected
Andere Namen:
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Sonstiges: Exercise + Rapid Weight Loss
Participants underwent a 5-day rapid weight loss protocol prior to the exercise session, achieving an average body mass reduction of 4.3%.
On the fifth day, the same standardized exercise protocol as Group 1 was performed, and pre- and post-exercise blood samples were collected (pre-exercise: ARWL1; post-exercise: ARWL2) to evaluate hematological and lymphocyte subset responses.
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Standardized wrestling exercise session; pre- and post-exercise venous blood collected
Andere Namen:
5-day rapid weight loss protocol achieving average 4.3% body mass reduction
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Was misst die Studie?
Primäre Ergebnismessungen
Ergebnis Maßnahme |
Maßnahmenbeschreibung |
Zeitfenster |
|---|---|---|
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Change in Leukocyte Count
Zeitfenster: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in total white blood cell (WBC) count measured from pre- to post-exercise
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Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in Lymphocyte Percentage
Zeitfenster: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in percentage of lymphocytes relative to total WBC count, measured from pre- to post-exercise.
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Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in CD3+ T Cell Count
Zeitfenster: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in absolute count of CD3+ T lymphocytes measured from pre- to post-exercise.
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Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in CD4+ T Cell Count
Zeitfenster: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in absolute count of CD4+ helper T lymphocytes measured from pre- to post-exercise.
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Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in CD8+ T Cell Count
Zeitfenster: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in absolute count of CD8+ cytotoxic T lymphocytes measured from pre- to post-exercise.
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Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in CD19+ B Cell Count
Zeitfenster: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in absolute count of CD19+ B lymphocytes measured from pre- to post-exercise.
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Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in CD16/56+ NK Cell Count
Zeitfenster: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in absolute count of CD16/56+ natural killer (NK) cells measured from pre- to post-exercise.
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Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Sekundäre Ergebnismessungen
Ergebnis Maßnahme |
Maßnahmenbeschreibung |
Zeitfenster |
|---|---|---|
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Change in Creatine Kinase and Lactic Acid Levels
Zeitfenster: Pre-exercise and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Serum creatine kinase (CK) and lactic acid (LA) levels measured to assess anaerobic performance and muscle damage.
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Pre-exercise and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Mitarbeiter und Ermittler
Hier finden Sie Personen und Organisationen, die an dieser Studie beteiligt sind.
Studienaufzeichnungsdaten
Diese Daten verfolgen den Fortschritt der Übermittlung von Studienaufzeichnungen und zusammenfassenden Ergebnissen an ClinicalTrials.gov. Studienaufzeichnungen und gemeldete Ergebnisse werden von der National Library of Medicine (NLM) überprüft, um sicherzustellen, dass sie bestimmten Qualitätskontrollstandards entsprechen, bevor sie auf der öffentlichen Website veröffentlicht werden.
Haupttermine studieren
Studienbeginn (Tatsächlich)
21. Juni 2021
Primärer Abschluss (Tatsächlich)
3. April 2022
Studienabschluss (Tatsächlich)
10. Oktober 2022
Studienanmeldedaten
Zuerst eingereicht
22. April 2026
Zuerst eingereicht, das die QC-Kriterien erfüllt hat
28. April 2026
Zuerst gepostet (Tatsächlich)
5. Mai 2026
Studienaufzeichnungsaktualisierungen
Letztes Update gepostet (Tatsächlich)
15. Mai 2026
Letztes eingereichtes Update, das die QC-Kriterien erfüllt
13. Mai 2026
Zuletzt verifiziert
1. Mai 2026
Mehr Informationen
Begriffe im Zusammenhang mit dieser Studie
Schlüsselwörter
Zusätzliche relevante MeSH-Bedingungen
Andere Studien-ID-Nummern
- 2280
Plan für individuelle Teilnehmerdaten (IPD)
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Studiert ein von der US-amerikanischen FDA reguliertes Geräteprodukt
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