Effects of Exercise and Short Term Rapid Weight Loss on Whole Blood Cells and Lymphocyte Subsets in Highly Trained National Level Wrestlers (EX-RWL-Lymphoc)
13. mai 2026 oppdatert av: Ankara Yildirim Beyazıt University
Effects of Exercise and Short-Term Rapid Weight Loss on Whole Blood Cells and Lymphocyte Subsets in Highly Trained National-Level Wrestlers: A Two-Phase Repeated-Measures Study
Wrestling is a weight-classified sport in which athletes frequently apply rapid weight loss (RWL) strategies prior to competition. While the performance-related consequences of RWL have been widely investigated, its combined effects with exercise on hematological parameters and immune cell profiles remain insufficiently understood. Therefore, this study aims to examine the effects of short-term weight loss and exercise on whole blood cell parameters and lymphocyte subsets in elite wrestlers.
Nine elite male wrestlers actively competing in the senior category (mean age: 22.90 ± 5.15 years; mean height: 1.75 m) voluntarily participated in this study. Athletes will achieve an average body mass reduction of 4.3% over a five-day period. Venous blood samples will be collected at 09:30 a.m. in a fasted state during two experimental phases: (1) Exercise-only phase, with blood sampling before and after exercise (BRWL1 and BRWL2); (2) Exercise plus weight loss phase, following the five-day weight loss period, with blood sampling before and after exercise (ARWL1 and ARWL2). Complete blood count parameters will be analyzed using an automated hematology analyzer, while lymphocyte subsets (CD3+, CD4+, CD8+, CD19+, CD16/56+) will be assessed by flow cytometry. Statistical analyses will be performed with a significance level set at p < 0.05.
Studieoversikt
Status
Fullført
Intervensjon / Behandling
Detaljert beskrivelse
See brief summary for study protocol details
Studietype
Intervensjonell
Registrering (Faktiske)
9
Fase
- Ikke aktuelt
Kontakter og plasseringer
Denne delen inneholder kontaktinformasjon for de som utfører studien, og informasjon om hvor denne studien blir utført.
Studiesteder
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Ankara
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Ankara, Ankara, Tyrkia (Türkiye), 06000
- Ankara Yildirim Beyazit University Sports Sciences Laboratory
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Deltakelseskriterier
Forskere ser etter personer som passer til en bestemt beskrivelse, kalt kvalifikasjonskriterier. Noen eksempler på disse kriteriene er en persons generelle helsetilstand eller tidligere behandlinger.
Kvalifikasjonskriterier
Alder som er kvalifisert for studier
- Voksen
Tar imot friske frivillige
Ja
Beskrivelse
Inclusion Criteria:
- Being between 18 and 35 years of age
- Being clinically healthy
- Actively competing in wrestling
- Having regular training experience
- No history of chronic disease
- No musculoskeletal injury that could affect performance
- Not using any medication or nutritional supplements that could influence hematological or biochemical parameters during the study period
- Willing to volunteer and provide written informed consent
Exclusion Criteria:
- Having any chronic disease (e.g., cardiovascular, metabolic, respiratory, or neurological disorders)
- Having a recent musculoskeletal injury that could affect exercise performance
- Using medications, ergogenic aids, or nutritional supplements that might influence hematological or biochemical parameters
- Having a history of smoking or alcohol abuse
- Failing to complete the testing procedures and training protocol required for the study
- Not providing written informed consent
- Withdrawing voluntarily during the study period
Studieplan
Denne delen gir detaljer om studieplanen, inkludert hvordan studien er utformet og hva studien måler.
Hvordan er studiet utformet?
Designdetaljer
- Primært formål: Grunnvitenskap
- Tildeling: Ikke-randomisert
- Intervensjonsmodell: Sekvensiell tildeling
- Masking: Ingen (Open Label)
Våpen og intervensjoner
Deltakergruppe / Arm |
Intervensjon / Behandling |
|---|---|
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Annen: Exercise
Participants performed a standardized wrestling exercise session on the first day of testing.
Venous blood samples were collected pre- and post-exercise (pre-exercise: BRWL1; post-exercise: BRWL2) to evaluate hematological and lymphocyte subset responses.
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Standardized wrestling exercise session; pre- and post-exercise venous blood collected
Andre navn:
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Annen: Exercise + Rapid Weight Loss
Participants underwent a 5-day rapid weight loss protocol prior to the exercise session, achieving an average body mass reduction of 4.3%.
On the fifth day, the same standardized exercise protocol as Group 1 was performed, and pre- and post-exercise blood samples were collected (pre-exercise: ARWL1; post-exercise: ARWL2) to evaluate hematological and lymphocyte subset responses.
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Standardized wrestling exercise session; pre- and post-exercise venous blood collected
Andre navn:
5-day rapid weight loss protocol achieving average 4.3% body mass reduction
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Hva måler studien?
Primære resultatmål
Resultatmål |
Tiltaksbeskrivelse |
Tidsramme |
|---|---|---|
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Change in Leukocyte Count
Tidsramme: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in total white blood cell (WBC) count measured from pre- to post-exercise
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Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in Lymphocyte Percentage
Tidsramme: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in percentage of lymphocytes relative to total WBC count, measured from pre- to post-exercise.
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Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in CD3+ T Cell Count
Tidsramme: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in absolute count of CD3+ T lymphocytes measured from pre- to post-exercise.
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Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in CD4+ T Cell Count
Tidsramme: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in absolute count of CD4+ helper T lymphocytes measured from pre- to post-exercise.
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Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in CD8+ T Cell Count
Tidsramme: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in absolute count of CD8+ cytotoxic T lymphocytes measured from pre- to post-exercise.
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Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in CD19+ B Cell Count
Tidsramme: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in absolute count of CD19+ B lymphocytes measured from pre- to post-exercise.
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Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in CD16/56+ NK Cell Count
Tidsramme: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Change in absolute count of CD16/56+ natural killer (NK) cells measured from pre- to post-exercise.
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Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Sekundære resultatmål
Resultatmål |
Tiltaksbeskrivelse |
Tidsramme |
|---|---|---|
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Change in Creatine Kinase and Lactic Acid Levels
Tidsramme: Pre-exercise and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Serum creatine kinase (CK) and lactic acid (LA) levels measured to assess anaerobic performance and muscle damage.
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Pre-exercise and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
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Samarbeidspartnere og etterforskere
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Studierekorddatoer
Disse datoene sporer fremdriften for innsending av studieposter og sammendragsresultater til ClinicalTrials.gov. Studieposter og rapporterte resultater gjennomgås av National Library of Medicine (NLM) for å sikre at de oppfyller spesifikke kvalitetskontrollstandarder før de legges ut på det offentlige nettstedet.
Studer hoveddatoer
Studiestart (Faktiske)
21. juni 2021
Primær fullføring (Faktiske)
3. april 2022
Studiet fullført (Faktiske)
10. oktober 2022
Datoer for studieregistrering
Først innsendt
22. april 2026
Først innsendt som oppfylte QC-kriteriene
28. april 2026
Først lagt ut (Faktiske)
5. mai 2026
Oppdateringer av studieposter
Sist oppdatering lagt ut (Faktiske)
15. mai 2026
Siste oppdatering sendt inn som oppfylte QC-kriteriene
13. mai 2026
Sist bekreftet
1. mai 2026
Mer informasjon
Begreper knyttet til denne studien
Nøkkelord
Ytterligere relevante MeSH-vilkår
Andre studie-ID-numre
- 2280
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