Application of laser speckle contrast imaging in laparoscopic surgery
Wido Heeman, Klaas Dijkstra, Christiaan Hoff, Sietze Koopal, Jean-Pierre Pierie, Hessel Bouma, E Christiaan Boerma, Wido Heeman, Klaas Dijkstra, Christiaan Hoff, Sietze Koopal, Jean-Pierre Pierie, Hessel Bouma, E Christiaan Boerma
Abstract
Anastomotic leakage is a worldwide problem in gastrointestinal surgery which seems to be related to the state of microcirculation. Laser speckle contrast imaging (LSCI) could give surgeons insight in the state of microcirculation to attune the site of anastomosis. This work studies the feasibility of LSCI as a tool for this purpose. An experimental setup was developed using a commercially available laparoscopic video system. Laser speckle contrast imaging is capable of detecting ischemic areas on the large intestine. Further research and development are required before adaptation of this technique in the operating room.
Conflict of interest statement
Mr. Bouma reports financial support from LIMIS Development B.V. during the conduct of the study. The other authors declare that they have no conflict of interest.
Figures
Endoscopic laser speckle contrast imaging (LSCI) system setup. a. As used during colon resections and b. as used for the nail fold experiment.
a. The pseudo-color images of the vascular occlusion test on the colon. a1 and b1 show a color image of the large intestine with green and red laser light respectively. a2/b2-a6/b6 are perfusion maps of the region of interest found in a1 and b1. a2/b2 is during baseline, a3/b3 and a4/b4 are during occlusion, a5/b5 is during reperfusion and a6/b6 is during post occlusion. Red indicates high flow, blue indicates low flow. Black means over/under exposure. The brightness of b1 was increased for illustrative purposes. Also, the laser speckle contrast imaging values normalized on baseline as measured in the 150x150 pixel region of interest using c. a green laser (532nm, n = 9) and d. a red laser (680nm, n = 5) during the vascular occlusion test in the large intestines. Results are normalized using their own baseline level. *p Fig. 3 Fig. 4
Fig. 3
An ischemia-reperfusion trace found with…
Fig. 3
An ischemia-reperfusion trace found with laser speckle contrast imaging analysis after a short…
An ischemia-reperfusion trace found with laser speckle contrast imaging analysis after a short occlusion of ± 7 seconds on the large intestine.
Fig. 4
The pseudo-color images of the…
Fig. 4
The pseudo-color images of the vascular occlusion test on the nail fold. a1…
The pseudo-color images of the vascular occlusion test on the nail fold. a1 and b1 show a color image of the finger with green and red laser light respectively. a2/b2-a6/b6 are perfusion maps of the region of interest found in a1 and b1 for the green and red laser respectively. a2b/2 is during baseline, a3/b3 and a4/b4 are during occlusion, a5/b5 is during reperfusion and a6/b6 is during post occlusion. Red indicates high flow, blue indicates low flow. Black means over/under exposure. The brightness of b1 was increased for illustrative purposes. Also, the laser speckle contrast imaging values normalized on baseline as measured in the 150x150 pixel region of interest using c. a green laser (532nm, n = 10) and d. a red laser (680nm, n = 10) during the vascular occlusion test in the large intestines. Results are normalized using their own baseline level. *p
Similar articles
- Experimental evaluation of laparoscopic laser speckle contrast imaging to visualize perfusion deficits during intestinal surgery.Heeman W, Wildeboer ACL, Al-Taher M, Calon JEM, Stassen LPS, Diana M, Derikx JPM, van Dam GM, Boerma EC, Bouvy ND. Heeman W, et al. Surg Endosc. 2023 Feb;37(2):950-957. doi: 10.1007/s00464-022-09536-9. Epub 2022 Sep 6. Surg Endosc. 2023. PMID: 36068388 Free PMC article.
- Laparoscopic Laser Speckle Contrast Imaging Can Visualize Anastomotic Perfusion: A Demonstration in a Porcine Model.Wildeboer A, Heeman W, van der Bilt A, Hoff C, Calon J, Boerma EC, Al-Taher M, Bouvy N. Wildeboer A, et al. Life (Basel). 2022 Aug 16;12(8):1251. doi: 10.3390/life12081251. Life (Basel). 2022. PMID: 36013430 Free PMC article.
- Laser speckle contrast imaging for assessment of liver microcirculation.Sturesson C, Milstein DM, Post IC, Maas AM, van Gulik TM. Sturesson C, et al. Microvasc Res. 2013 May;87:34-40. doi: 10.1016/j.mvr.2013.01.004. Epub 2013 Feb 8. Microvasc Res. 2013. PMID: 23403398
- Monitoring of partial and full venous outflow obstruction in a porcine flap model using laser speckle contrast imaging.Zötterman J, Bergkvist M, Iredahl F, Tesselaar E, Farnebo S. Zötterman J, et al. J Plast Reconstr Aesthet Surg. 2016 Jul;69(7):936-43. doi: 10.1016/j.bjps.2016.02.015. Epub 2016 Mar 10. J Plast Reconstr Aesthet Surg. 2016. PMID: 27026039
- Assessment of microcirculation in the diabetic foot with laser speckle contrast imaging.Mennes OA, van Netten JJ, van Baal JG, Steenbergen W. Mennes OA, et al. Physiol Meas. 2019 Jul 1;40(6):065002. doi: 10.1088/1361-6579/ab2058. Physiol Meas. 2019. PMID: 31071696
Cited by
- Low FODMAP Diet Relieves Visceral Hypersensitivity and Is Associated with Changes in Colonic Microcirculation in Water Avoidance Mice Model.Hu C, Yan C, Wu Y, Tao E, Guo R, Zhu Z, Chen X, Fang M, Jiang M. Hu C, et al. Nutrients. 2023 Feb 24;15(5):1155. doi: 10.3390/nu15051155. Nutrients. 2023. PMID: 36904154 Free PMC article.
- Real-time quantification of intestinal perfusion and arterial versus venous occlusion using laser speckle contrast imaging in porcine model.Liu YZ, Mehrotra S, Nwaiwu CA, Buharin VE, Oberlin J, Stolyarov R, Schwaitzberg SD, Kim PCW. Liu YZ, et al. Langenbecks Arch Surg. 2023 Mar 2;408(1):114. doi: 10.1007/s00423-023-02845-0. Langenbecks Arch Surg. 2023. PMID: 36859714
- Real-time, multi-spectral motion artefact correction and compensation for laser speckle contrast imaging.Heeman W, Maassen H, Dijkstra K, Calon J, van Goor H, Leuvenink H, van Dam GM, Boerma EC. Heeman W, et al. Sci Rep. 2022 Dec 15;12(1):21718. doi: 10.1038/s41598-022-26154-6. Sci Rep. 2022. PMID: 36522524 Free PMC article.
- Unsupervised and quantitative intestinal ischemia detection using conditional adversarial network in multimodal optical imaging.Wang Y, Tiusaba L, Jacobs S, Saruwatari M, Ning B, Levitt M, Sandler AD, Nam SH, Kang JU, Cha J. Wang Y, et al. J Med Imaging (Bellingham). 2022 Nov;9(6):064502. doi: 10.1117/1.JMI.9.6.064502. Epub 2022 Nov 28. J Med Imaging (Bellingham). 2022. PMID: 36466077
- Utility and usability of laser speckle contrast imaging (LSCI) for displaying real-time tissue perfusion/blood flow in robot-assisted surgery (RAS): comparison to indocyanine green (ICG) and use in laparoscopic surgery.Liu YZ, Shah SK, Sanders CM, Nwaiwu CA, Dechert AF, Mehrotra S, Schwaitzberg SD, Kim PCW, Wilson EB. Liu YZ, et al. Surg Endosc. 2022 Sep 15:1-9. doi: 10.1007/s00464-022-09590-3. Online ahead of print. Surg Endosc. 2022. PMID: 36109357 Free PMC article.
LinkOut - more resources
- Full Text Sources
- Medical
Full text links [x]
[x]
Cite
Copy Download .nbib
Format: AMA APA MLA NLM
Send To [x]
An ischemia-reperfusion trace found with laser speckle contrast imaging analysis after a short occlusion of ± 7 seconds on the large intestine.
The pseudo-color images of the vascular occlusion test on the nail fold. a1 and b1 show a color image of the finger with green and red laser light respectively. a2/b2-a6/b6 are perfusion maps of the region of interest found in a1 and b1 for the green and red laser respectively. a2b/2 is during baseline, a3/b3 and a4/b4 are during occlusion, a5/b5 is during reperfusion and a6/b6 is during post occlusion. Red indicates high flow, blue indicates low flow. Black means over/under exposure. The brightness of b1 was increased for illustrative purposes. Also, the laser speckle contrast imaging values normalized on baseline as measured in the 150x150 pixel region of interest using c. a green laser (532nm, n = 10) and d. a red laser (680nm, n = 10) during the vascular occlusion test in the large intestines. Results are normalized using their own baseline level. *p
Source: PubMed