Efficacy of Intrauterine infusion of granulocyte colony stimulating factor on patients with history of implantation failure: A randomized control trial
Maryam Eftekhar, Sepideh Miraj, Maryam Farid Mojtahedi, Nosrat Neghab, Maryam Eftekhar, Sepideh Miraj, Maryam Farid Mojtahedi, Nosrat Neghab
Abstract
Background: Although pregnancy rate in in vitro fertilization-embryo transfer (IVF-ET) cycles has been increased over the preceding years, but the majority of IVF-ET cycles still fail. Granulocyte colony stimulating factor (GCSF) is a glycoprotein that stimulates cytokine growth factor and induces immune system which may improve pregnancy rate in women with history of implantation failure.
Objective: The aim of this study was to evaluate GCSF ability to improve pregnancy rate in women with history of implantation failure.
Materials and methods: 0.5 ml (300 µg/ml) GCSF was infused intrauterine in intervention group. Pregnancy outcomes were assessed based on clinical pregnancy.
Results: The mean age of participants was 31.95±4.71 years old. There were no significant differences between demographic characteristics in two groups (p>0.05). The pregnancy outcome in GCSF group was improved significantly (p=0.043).
Conclusion: GCSF can improve pregnancy outcome in patients with history of implantation failure.
Keywords: GCSF; In vitro fertilization; Pregnancy rate.
Conflict of interest statement
The authors declare that there was no conflict of interests regarding the publication of this article.
Figures
References
- Richter KS, Bugge KR, Bromer JG, Levy MJ. Relationship between endometrial thickness and embryo implantation, based on 1,294 cycles of in vitro fertilization with transfer of two blastocyst-stage embryos. Fertil Steril. 2007;87:53–59.
- Eftekhar M, Hosseinisadat R, Baradaran R, Naghshineh E. Effect of granulocyte colony stimulating factor (G-CSF) on IVF outcomes in infertile women: An RCT. Int J Reprod Biomed. 2015;14:341–346.
- Traub ML, Van Arsdale A, Pal L, Jindal S, Santoro N. Endometrial thickness, Caucasian ethnicity, and age predict clinical pregnancy following fresh blastocyst embryo transfer: a retrospective cohort. Reprod Biol Endocrinol. 2009;7:27–31.
- Gonen Y, Casper RF. Prediction of implantation by the sonographic appearance of the endometrium during controlled ovarian stimulation for in vitro fertilization (IVF) J In Vitro Fertil Embryo Transfer. 1990;7:146–152.
- Margalioth E, Ben-Chetrit A, Gal M, Eldar-Geva T. Investigation and treatment of repeated implantation failure following IVF-ET. Hum Reprod. 2006;21:3036–3043.
- Shufaro Y, Schenker JG. Implantation failure, etiology, diagnosis and treatment. Int J Infertil Fetal Med. 2011;2:1–7.
- Margalioth EJ, Ben-Chetrit A, Gal M, Eldar-Geva T. Investigation and treatment of repeated implantation failure following IVF-ET. Hum Reprod. 2006;21:3036–3043.
- Isaacs Jr J, Wells C, Williams D, Odem R, Gast M, Strickler R. Endometrial thickness is a valid monitoring parameter in cycles of ovulation induction with menotropins alone. Fertil Steril. 1996;65:262–266.
- Fiedler K, Wurfel W. Effectivity of heparin in assisted reproduction. Eur J Med Res. 2004;9:207–214.
- Broekmans FJ, Kwee J, Hendriks DJ, Mol BW, Lambalk CB. A systematic review of tests predicting ovarian reserve and IVF outcome. Hum Reprod Update. 2006;12:685–718.
- Al-Ghamdi A, Coskun S, Al-Hassan S, Al-Rejjal R, Awartani K. The correlation between endometrial thickness and outcome of in vitro fertilization and embryo transfer (IVF-ET) outcome. Reprod Biol Endocrinol. 2008;6:37.
- Salmassi A, Schmutzler AG, Schaefer S, Koch K, Hedderich J, Jonat W, et al. Is granulocyte colony-stimulating factor level predictive for human IVF outcome? Hum Reprod. 2005;20:2434–2440.
- Rutella S, Zavala F, Danese S, Kared H, Leone G. Granulocyte colony-stimulating factor: a novel mediator of T cell tolerance. J Immunol. 2005;175:7085–7091.
- Allahbadia G. The unfinished story of granulocyte colony-stimulating factor in assisted reproductive technology. IVF Lite. 2014;1:139.
- Wang H, Wen Y, Polan ML, Boostanfar R, Feinman M, Behr B. Exogenous granulocyte-macrophage colony-stimulating factor promotes follicular development in the newborn rat in vivo. Hum Reprod. 2005;20:2749–2756.
- Scarpellini F, Sbracia M. Use of granulocyte colony-stimulating factor for the treatment of unexplained recurrent miscarriage: a randomised controlled trial. Hum Reprod. 2009;24:2703–2708.
- Okohue J, Onuh S, Ebeigbe P, Shaibu I, Wada I, Ikimalo J, et al. The effect of endometrial thickness on in vitro fertilization (IVF)-embryo transfer/ intracytoplasmic sperm injection (ICSI) outcome. Afr J Reprod Health. 2009;13:113–121.
- Gleicher N, Vidali A, Barad DH. Successful treatment of unresponsive thin endometrium. Fertil Steril. 2011;95:2123.
- Gleicher N, Kim A, Michaeli T, Lee H, Shohat-Tal A, Lazzaroni E, et al. A pilot cohort study of granulocyte colony-stimulating factor in the treatment of unresponsive thin endometrium resistant to standard therapies. Hum Reprod. 2013;28:172–177.
- Eftekhar M, Sayadi M, Arabjahvani F. Transvaginal perfusion of G-CSF for infertile women with thin endometrium in frozen ET program: A non-randomized clinical trial. Iran J Reprod Med. 2014;12:661–666.
- Wang H, Wen Y, Polan ML, Boostanfar R, Feinman M, Behr B. Exogenous granulocyte-macrophage colony-stimulating factor promotes follicular development in the newborn rat in vivo. Hum Reprod. 2005;20:2749–2756.
- Barad DH, Yu Y, Kushnir VA, Shohat-Tal A, Lazzaroni E, Lee HJ, et al. A randomized clinical trial of endometrial perfusion with granulocyte colony-stimulating factor in in vitro fertilization cycles: impact on endometrial thickness and clinical pregnancy rates. Fertil Steril. 2014;101:710–715.
- Kunicki M, Lukaszuk K, Woclawek-Potocka I, Liss J, Kulwikowska P, Szczyptanska J. Evaluation of granulocyte colony-stimulating factor effects on treatment-resistant thin endometrium in women undergoing in vitro fertilization. BioMed Res Int. 2014;2014:913235.
- Sjoblom C, Wikland M, Robertson SA. Granulocyte-macrophage colony-stimulating factor promotes human blastocyst development in vitro. Hum Reprod. 1999;14:3069–3076.
Source: PubMed