Estimating Chikungunya prevalence in La Réunion Island outbreak by serosurveys: two methods for two critical times of the epidemic

Abstract

Background: Chikungunya virus (CHIKV) caused a major two-wave seventeen-month-long outbreak in La Réunion Island in 2005-2006. The aim of this study was to refine clinical estimates provided by a regional surveillance-system using a two-stage serological assessment as gold standard.

Methods: Two serosurveys were implemented: first, a rapid survey using stored sera of pregnant women, in order to assess the attack rate at the epidemic upsurge (s1, February 2006; n = 888); second, a population-based survey among a random sample of the community, to assess the herd immunity in the post-epidemic era (s2, October 2006; n = 2442). Sera were screened for anti-CHIKV specific antibodies (IgM and IgG in s1, IgG only in s2) using enzyme-linked immunosorbent assays. Seroprevalence rates were compared to clinical estimates of attack rates.

Results: In s1, 18.2% of the pregnant women were tested positive for CHIKV specific antibodies (13.8% for both IgM and IgG, 4.3% for IgM, 0.1% for IgG only) which provided a congruent estimate with the 16.5% attack rate calculated from the surveillance-system. In s2, the seroprevalence in community was estimated to 38.2% (95% CI, 35.9 to 40.6%). Extrapolations of seroprevalence rates led to estimate, at 143,000 and at 300,000 (95% CI, 283,000 to 320,000), the number of people infected in s1 and in s2, respectively. In comparison, the surveillance-system estimated at 130,000 and 266,000 the number of people infected for the same periods.

Conclusion: A rapid serosurvey in pregnant women can be helpful to assess the attack rate when large seroprevalence studies cannot be done. On the other hand, a population-based serosurvey is useful to refine the estimate when clinical diagnosis underestimates it. Our findings give valuable insights to assess the herd immunity along the course of epidemics.

Figures

Figure 1

Map of La Réunion Island. The territory is divided into four regions: north bounded by orange and red lines, west bounded by orange, light green, dark green and red lines, south bounded by dark green and red lines, east by red lines. For each municipality, the ratio of laboratories which participated to the survey on pregnant woman and the related amount of the sera collected (n = x) are listed in parentheses.

Figure 2

Number of weekly incident cases of Chikungunya, La Réunion Island, March 28th, 2005 – April 16th, 2006 (n = 244,000). Reported by the active case-finding system between weeks 9 and 50, 2005 or estimated from the sentinel physician network between week 51 of 2005 and week 15, 2006. Published by Renault P, et al. in Am J Trop Med Hyg, 2007, 77: 727–731 [11], and reprinted with the kind permission of the American Society of Tropical Medicine and Hygiene (Atlanta, USA). "Survey 1" corresponds to the rapid serological survey on pregnant women (January 15th 2006 to February 15th 2006); "Survey 2" corresponds to the population-based SEROCHIK survey (August 17th to October 20th 2006).

Figure 3

Comparison of monthly suspected, self-reported and confirmed cases of Chikungunya in La Réunion Island between April 2005 and October 2006. The number of suspected cases recorded weekly by the CIRE (left scale) is compared to the number of cases identified in the population-based SEROCHIK survey (right scale). For the serosurvey, both self-reports (all subjects who have declared that they have been infected, without taking into account serology results) and confirmed self-reports (with a positive serology) are noted. We refer to the date of first clinical signs declared by the subjects during the survey conducted between August 17th 2006 and October 20th 2006. "Suspected cases" are defined as cases with a sudden onset of fever with temperature > 38.5°C and incapacitating arthralgia.