Vitamin D and Mitosis Evaluation in Endometriosis: A Step toward Discovering the Connection?

Daniela Roxana Matasariu, Cristina Elena Mandici, Alexandra Ursache, Alexandra Irma Gabriela Bausic, Iuliana Elena Bujor, Alexandra Elena Cristofor, Lucian Vasile Boiculese, Mihaela Grigore, Elvira Bratila, Ludmila Lozneanu, Daniela Roxana Matasariu, Cristina Elena Mandici, Alexandra Ursache, Alexandra Irma Gabriela Bausic, Iuliana Elena Bujor, Alexandra Elena Cristofor, Lucian Vasile Boiculese, Mihaela Grigore, Elvira Bratila, Ludmila Lozneanu

Abstract

(1) Background: The effects of serum vitamin D levels, the vitamin D receptor (VDR), and phosphohistone H3 (PHH3) in endometriosis were investigated in two cohorts of women with this pathology: those receiving hormonal treatment and those without treatment. (2) Methods: In 60 cases of women with endometriosis (26 with progestin treatment and 34 without), paraffin-embedded endometriosis tissue samples retrieved after surgery were immunohistochemically (IHC) analyzed to determine the expression statuses of VDR and PHH3. In addition, serum levels of 25(OH) vitamin D were assessed for each patient. (3) Results: The serum 25(OH) vitamin D evaluations revealed higher levels of 25(OH) vitamin D in women with treatment compared with those without. The positive IHC indexes of VDR and PHH3 in these two groups were compared. Vitamin D receptor levels were positively correlated with PHH3 levels, both being increased in patients without treatment. (4) Conclusions: Serum 25(OH) vitamin D levels and IHC analysis of VDR and PHH3 can be used as additional tools for risk stratification and prognostic assessment in patients with endometriosis.

Keywords: 25(OH) vitamin D; diagnosis; dienogest; endometriosis; immunohistochemistry; phosphohistone H3; progestin; risk of endometriosis; vitamin D receptor.

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Immunohistochemical expression of VDR and PHH3 in two groups (with and without treatment).

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