Citrate Anticoagulant Improves the Sensitivity of Borreliella (Borrelia) burgdorferi Plasma Culture

Adriana R Marques, Xiuli Yang, Alexis A Smith, Xuran Zhuang, Siu-Ping Turk, Carla D Williams, Melissa A Law, Alan G Barbour, Utpal Pal, Adriana R Marques, Xiuli Yang, Alexis A Smith, Xuran Zhuang, Siu-Ping Turk, Carla D Williams, Melissa A Law, Alan G Barbour, Utpal Pal

No abstract available

Keywords: Borreliella (Borrelia) burgdorferi; Lyme disease; blood; blood culture; culture; laboratory diagnosis.

Figures

FIG 1

B. burgdorferi growth in BSK-H media from EDTA or citrate plasma samples. (A) Kaplan-Meier time to event curve for positive B. burgdorferi cultures from EDTA or citrate plasma samples. Comparisons of the curves by the log rank (Mantel-Cox) test showed a significant difference, with a P value of <0.0001. (B) Table showing the results from the 3 separate experiments. Experiment 1 compared EDTA and citrate samples processed after a 12-h interval. Experiment 2 examined samples processed within a 3-h interval. Experiment 3 examined both intervals. The columns show the number of positive tested samples/total number of tested samples. Culture vials were examined until positive results were obtained and/or until day 28. ND, not done.