Detection of Enterovirus RNA in Atheromatous Lesions (ATH-ENTE)
Detection of Enterovirus RNA by RT-PCR in Atheromatous Lesions
調査の概要
詳細な説明
Objective: In order to complete these data and to answer clearly to the question of the role of persistent infection by enteroviruses in atherosclerosis, we propose this research program involving the Departments of Vascular Surgery of three University hospitals of the "Rhône-Alpes" region and of three laboratories of Virology interested by the relationship between chronic diseases and enterovirus infection..
Methods: It is projected to include about one hundred voluntary adults exhibiting carotid atheromatous lesions requiring a surgical removal. Because of the difficulty to define a pertinent control group, each patient will be his/her own control. In addition to the fragment of pathologic carotid, it will be taken arterial and venous fragments from vessels that are never the seat of atherosclerosis lesions. To reach the best sensitivity, enterovirus RNA will be detected by nested RT-PCR.
Evaluation criteria: The main evaluation criterion will be the percentage of detection of enterovirus RNA by RT-PCR in atheromatous lesions by comparison to control arteries by Mac-Nemar test. These results will be correlated to anatomic and histological lesions of the atheromatous plaques. Other markers will be analysed secondarily: detection of enterovirus RNA in control venous samples, detection of stigmata of viral replication by immunochemistry or detection of negative RNA strands, analysis of the antibody response to enterovirus in blood, molecular typing of enteroviruses detected in the lesions… Expected benefits: This study should allow to better understand the putative involvement of enteroviruses in atherosclerosis-linked diseases. If the hypothesis of a relationship was confirmed, a more ambitious research project would be proposed in order to investigate the mechanisms by which enterovirus can favour atherosclerosis and to discuss the opportunity of preventive strategies (notably vaccination) to reduce the risk.
研究の種類
入学 (実際)
段階
- 適用できない
連絡先と場所
研究場所
-
-
-
Clermont-ferrand、フランス、63000
- CHU de Clermont-Ferrand
-
Grenoble、フランス、38000
- CHU de Grenoble
-
Saint-etienne、フランス、42055
- CHU de Saint-Etienne
-
-
参加基準
適格基準
就学可能な年齢
健康ボランティアの受け入れ
受講資格のある性別
説明
Inclusion Criteria:
- Indication of artery resection
- affiliated or a beneficiary of a social security category
- having signed the inform consent form
Exclusion Criteria:
- pregnant women
- contra-indication to clamping
- scheduled resection at friday after-noon, week-end or legal holidays
研究計画
研究はどのように設計されていますか?
デザインの詳細
- 主な目的:基礎科学
- 割り当て:なし
- 介入モデル:単一グループの割り当て
- マスキング:なし(オープンラベル)
武器と介入
参加者グループ / アーム |
介入・治療 |
---|---|
他の:1
Enterovirus RNA analysis
|
Artery resection
|
この研究は何を測定していますか?
主要な結果の測定
結果測定 |
時間枠 |
---|---|
Prévalence of detection of enteroviral RNA by RT-PCR in atherosclerotic carotid artery in comparison with sound carotid artery
時間枠:artery resection
|
artery resection
|
二次結果の測定
結果測定 |
時間枠 |
---|---|
Prevalence of detection of enteroviral RNA by RT-PCR in atherosclerotic carotid artery in comparison with sound vein
時間枠:Artery resection
|
Artery resection
|
Prevalence of detection of enteroviral RNA by RT-PCR in sound carotid artery in comparison with sound vein
時間枠:Artery resection
|
Artery resection
|
Prevalence of detection of enteroviral RNA by RT-PCR in calm and active atherosclerotic lesions
時間枠:Artery resection
|
Artery resection
|
Prevalence of detection of antibodies between different groups of patients.
時間枠:Artery resection
|
Artery resection
|
Prevalence of detection of enteroviral RNA by RT-PCR in atherosclerotic carotid artery in comparison with circulating blood
時間枠:Artery resection
|
Artery resection
|
協力者と研究者
捜査官
- 主任研究者:Xavier BARRAL, MD PhD、CHU de Saint-Etienne
出版物と役立つリンク
一般刊行物
- Schanen C, Nasri D, Bourlet T, Barral X, Favre JP, Bourrat D, Peoc'h M, Ginevra C, Andreoletti L, Pozzetto B, Pillet S. Enterovirus in arteriosclerosis: a pilot study. J Clin Virol. 2007 Jun;39(2):106-12. doi: 10.1016/j.jcv.2007.03.014. Epub 2007 May 10.
- Andreoletti L, Venteo L, Douche-Aourik F, Canas F, Lorin de la Grandmaison G, Jacques J, Moret H, Jovenin N, Mosnier JF, Matta M, Duband S, Pluot M, Pozzetto B, Bourlet T. Active Coxsackieviral B infection is associated with disruption of dystrophin in endomyocardial tissue of patients who died suddenly of acute myocardial infarction. J Am Coll Cardiol. 2007 Dec 4;50(23):2207-14. doi: 10.1016/j.jacc.2007.07.080. Epub 2007 Nov 19.
研究記録日
主要日程の研究
研究開始
一次修了 (実際)
研究の完了 (実際)
試験登録日
最初に提出
QC基準を満たした最初の提出物
最初の投稿 (見積もり)
学習記録の更新
投稿された最後の更新 (見積もり)
QC基準を満たした最後の更新が送信されました
最終確認日
詳しくは
本研究に関する用語
キーワード
追加の関連 MeSH 用語
その他の研究ID番号
- 0608071
- 2007-A00895-48 (その他の識別子:AFSSAPS)
- DGS2007-0534 (その他の識別子:DGS)
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