Assessment of Early Genetic Changes in Smokers
研究概览
详细说明
Our pilot study using cellular DNA (cDNA) microarrays to examine the buccal mucosa of smokers and non-smokers demonstrated that smokers could be separated from non-smokers based solely on the patterns of gene expression observed. We were able to identify 924 genes whose expression differs significantly between samples from smokers and non-smokers. Several genes were also shown to be either up or down regulated in our earlier research applying microarray analysis to head and neck cancer tumors. Many of these represent genes of possible interest as early molecular markers for head and neck carcinogenesis.
Aberrant methylation is an important event in the transcriptional silencing of candidate tumor suppressor genes in smoking associated malignancies. Furthermore, it is known that methylated CpG islands are the preferred binding site for benzo(a)pyrene diol epoxide and other carcinogens found in tobacco smoke. Binding of these compounds is known to cause DNA adducts and transversion mutations that are often observed in the aerodigestive tumors of smokers. New evidence suggests that specific DNA methylation events are directly linked to tobacco use. The ability to detect such molecular markers during screening of high risk groups would represent a significant advance in cancer screening and early detection. Our group has evaluated specimens to epigenetically profile CpG island hypermethylation in. head and neck squamous cell carcinoma ( HNSCC) tumor samples using a technique known as methylation specific restriction enzyme microarray analysis. This method will be used in this trial to detect alterations in global DNA methylation patterns in subjects who smoke compared to those who don't.
The objectives of this study are:
- Test the hypothesis that there are specific genetic alterations, leading to gene expression profile changes, which will be detected in early smokers.
- Test the hypothesis that early smokers will demonstrate alterations in global DNA methylation patterns compared to matched controls.
- To analyze gene alterations and DNA methylation in college smokers over time through longitudinal follow-up.
研究类型
注册 (实际的)
联系人和位置
学习地点
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New York
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Bronx、New York、美国、10467
- Montefiore Medical Center
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参与标准
资格标准
适合学习的年龄
接受健康志愿者
有资格学习的性别
取样方法
研究人群
描述
Inclusion Criteria:
College freshmen and sophomores
Smokers must be between the ages of 18 and 25
Smokers must have smoked regularly for at least 2 years and be currently smoking
Smokers must intend to stay in the New York area for at least 3 years.
Non-smokers must be non-users of marijuana -
Exclusion Criteria:
Current HIV/AIDS infection
Use of chewing tobacco
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学习计划
研究是如何设计的?
设计细节
- 观测模型:病例对照
- 时间观点:预期
队列和干预
团体/队列 |
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smokers
college students who smoke
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non smokers
college students who don't smoke
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研究衡量的是什么?
主要结果指标
结果测量 |
措施说明 |
大体时间 |
---|---|---|
alteration of gene expression profile
大体时间:2 yrs
|
comparison of gene expression profiles between smokers and non-smokers
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2 yrs
|
合作者和调查者
调查人员
- 学习椅:Richard V Smith, MD、Montefiore Medical Center
- 首席研究员:Thomas Belbin, PhD、Albert Einstein College of Medicine
- 首席研究员:Nicholas Schlecht, PhD、Albert Einstein College of Medicine
研究记录日期
研究主要日期
学习开始
初级完成 (实际的)
研究注册日期
首次提交
首先提交符合 QC 标准的
首次发布 (估计)
研究记录更新
最后更新发布 (估计)
上次提交的符合 QC 标准的更新
最后验证
更多信息
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