Special Stains in Assessment of Bone Marrow Fibrosis
Comparative Evaluation of Special Stains in Assessment of Bone Marrow Fibrosis
This cross-sectional study aims to compare Picrosirius Red, Masson's Trichrome, and Reticulin stains for the detection and assessment of bone marrow fibrosis in trephine biopsy specimens.
研究概览
地位
条件
详细说明
Bone marrow fibrosis is assessed using special stains that highlight reticulin and collagen fibers. This observational study will evaluate formalin-fixed paraffin-embedded bone marrow trephine biopsy specimens.
Three serial sections from each specimen will be stained with Reticulin, Masson's Trichrome, and Picrosirius Red. Fibrosis will be graded using the MF-0 to MF-3 system, and findings will be compared across the three staining methods.
研究类型
注册 (估计的)
联系人和位置
学习联系方式
- 姓名：Salma M Fathallah, Resident
- 电话号码：01066296349
- 邮箱：salma.18313350@med.aun.edu.eg
参与标准
资格标准
适合学习的年龄
- 孩子
- 成人
- 年长者
接受健康志愿者
有资格学习的性别
取样方法
研究人群
Bone marrow trephine biopsy specimens with suspected or established marrow fibrosis processed at South Egypt Cancer Institute, Assiut University.
描述
Inclusion Criteria:
- - Adequate bone marrow trephine biopsy tissue with sufficient well-fixed, well-preserved paraffin-embedded material for all required stains
- Cases with suspected or established marrow fibrosis and variable degrees of fibrosis
- Availability of relevant clinical and pathological information
Exclusion Criteria:
- - Inadequate, fragmented, poorly fixed, or autolyzed tissue
- Unsuitable paraffin blocks due to processing artifacts
- Missing or insufficient clinical or pathological data required for analysis
学习计划
研究是如何设计的？
设计细节
- 观测模型：CASE_ONLY
- 时间观点：CROSS_SECTIONAL
队列和干预
团体/队列 |
|---|
|
Single cohort: Bone Marrow Biopsy Specimens
Formalin-fixed paraffin-embedded bone marrow trephine biopsy specimens that will be stained with Reticulin, Masson's Trichrome, and Picrosirius Red for fibrosis assessment. |
研究衡量的是什么？
主要结果指标
结果测量 |
措施说明 |
大体时间 |
|---|---|---|
|
Mean reticulin fibrosis grade
大体时间：Baseline
|
Mean reticulin fibrosis grade according to the European consensus MF-0 to MF-3 system. |
Baseline
|
次要结果测量
结果测量 |
措施说明 |
大体时间 |
|---|---|---|
|
Mean collagen deposition grade by Masson's Trichrome
大体时间：Baseline
|
Mean semiquantitative grade of collagen deposition assessed by Masson's Trichrome stain. |
Baseline
|
|
Mean collagen deposition grade by Picrosirius Red
大体时间：Baseline
|
Mean semiquantitative grade of collagen deposition assessed by Picrosirius Red stain. |
Baseline
|
|
Proportion of specimens with MF-3 fibrosis
大体时间：Baseline
|
Proportion of bone marrow specimens graded as MF-3 by reticulin stain. |
Baseline
|
|
Proportion of specimens with concordant grades between Reticulin and Picrosirius Red
大体时间：Baseline
|
Proportion of specimens showing concordant fibrosis grades between Reticulin and Picrosirius Red stains. |
Baseline
|
合作者和调查者
调查人员
- 学习椅：Eman M Zaki, prof、Oncological Clinical Pathology Department, Assiut University Hospitals
研究记录日期
研究主要日期
学习开始 (估计的)
初级完成 (估计的)
研究完成 (估计的)
研究注册日期
首次提交
首先提交符合 QC 标准的
首次发布 (实际的)
研究记录更新
最后更新发布 (实际的)
上次提交的符合 QC 标准的更新
最后验证
更多信息
与本研究相关的术语
其他研究编号
- BMF-Stains-Assiut-2026
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计划共享个人参与者数据 (IPD)？
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