Changes in Serum Free Amino Acids and Muscle Fatigue Experienced during a Half-Ironman Triathlon

Francisco Areces, Cristina González-Millán, Juan José Salinero, Javier Abian-Vicen, Beatriz Lara, Cesar Gallo-Salazar, Diana Ruiz-Vicente, Juan Del Coso, Francisco Areces, Cristina González-Millán, Juan José Salinero, Javier Abian-Vicen, Beatriz Lara, Cesar Gallo-Salazar, Diana Ruiz-Vicente, Juan Del Coso

Abstract

The aim of this study was to investigate the relationship between changes in serum free amino acids, muscle fatigue and exercise-induced muscle damage during a half-ironman triathlon. Twenty-six experienced triathletes (age = 37.0 ± 6.8 yr; experience = 7.4 ± 3.0 yr) competed in a real half-ironman triathlon in which sector times and total race time were measured by means of chip timing. Before and after the race, a countermovement jump and a maximal isometric force test were performed, and blood samples were withdrawn to measure serum free amino acids concentrations, and serum creatine kinase levels as a blood marker of muscle damage. Total race time was 320 ± 37 min and jump height (-16.3 ± 15.2%, P < 0.001) and isometric force (-14.9 ± 9.8%; P = 0.007) were significantly reduced after the race in all participants. After the race, the serum concentration of creatine kinase increased by 368 ± 187% (P < 0.001). In contrast, the serum concentrations of essential (-27.1 ± 13.0%; P < 0.001) and non-essential amino acids (-24.4 ± 13.1%; P < 0.001) were significantly reduced after the race. The tryptophan/BCAA ratio increased by 42.7 ± 12.7% after the race. Pre-to-post changes in serum free amino acids did not correlate with muscle performance variables or post-race creatine kinase concentration. In summary, during a half-ironman triathlon, serum amino acids concentrations were reduced by > 20%. However, neither the changes in serum free amino acids nor the tryptophan/BCAA ratio were related muscle fatigue or muscle damage during the race.

Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Fig 1. A) Relationship between race time…
Fig 1. A) Relationship between race time and sector times (swimming, cycling and running) and post-race tryptophan/BCAA ratio during a half-ironman race. B) Relationship between post-race creatine kinase concentration and changes of BCAA concentration during a half-ironman race (as a change from pre-exercise values).
Note: A) The correlation of total race time (r = -0.05; P = 0.80), swimming time (r = -0.05; P = 0.80), cycling time (r = 0.08; P = 0.70) and running time (r = -0.21; P = 0.30) with post-race tryptophan/BCAA ratio were not statistically significant. B) The correlation of post-race creatine kinase concentration with BCAA change during the race (r = -0.02; P = 0.92) was not statistically significant.

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Source: PubMed

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