Changes in Serum Free Amino Acids and Muscle Fatigue Experienced during a Half-Ironman Triathlon
Francisco Areces, Cristina González-Millán, Juan José Salinero, Javier Abian-Vicen, Beatriz Lara, Cesar Gallo-Salazar, Diana Ruiz-Vicente, Juan Del Coso, Francisco Areces, Cristina González-Millán, Juan José Salinero, Javier Abian-Vicen, Beatriz Lara, Cesar Gallo-Salazar, Diana Ruiz-Vicente, Juan Del Coso
Abstract
The aim of this study was to investigate the relationship between changes in serum free amino acids, muscle fatigue and exercise-induced muscle damage during a half-ironman triathlon. Twenty-six experienced triathletes (age = 37.0 ± 6.8 yr; experience = 7.4 ± 3.0 yr) competed in a real half-ironman triathlon in which sector times and total race time were measured by means of chip timing. Before and after the race, a countermovement jump and a maximal isometric force test were performed, and blood samples were withdrawn to measure serum free amino acids concentrations, and serum creatine kinase levels as a blood marker of muscle damage. Total race time was 320 ± 37 min and jump height (-16.3 ± 15.2%, P < 0.001) and isometric force (-14.9 ± 9.8%; P = 0.007) were significantly reduced after the race in all participants. After the race, the serum concentration of creatine kinase increased by 368 ± 187% (P < 0.001). In contrast, the serum concentrations of essential (-27.1 ± 13.0%; P < 0.001) and non-essential amino acids (-24.4 ± 13.1%; P < 0.001) were significantly reduced after the race. The tryptophan/BCAA ratio increased by 42.7 ± 12.7% after the race. Pre-to-post changes in serum free amino acids did not correlate with muscle performance variables or post-race creatine kinase concentration. In summary, during a half-ironman triathlon, serum amino acids concentrations were reduced by > 20%. However, neither the changes in serum free amino acids nor the tryptophan/BCAA ratio were related muscle fatigue or muscle damage during the race.
Conflict of interest statement
Competing Interests: The authors have declared that no competing interests exist.
Figures
![Fig 1. A) Relationship between race time…](https://www.ncbi.nlm.nih.gov/pmc/articles/instance/4570672/bin/pone.0138376.g001.jpg)
References
- Millet GP, Bentley DJ, Vleck VE (2007) The relationships between science and sport: application in triathlon. Int J Sports Physiol Perform 2: 315–322.
- Del Coso J, Gonzalez C, Abian-Vicen J, Salinero Martin JJ, Soriano L, Areces F, et al. (2014) Relationship between physiological parameters and performance during a half-ironman triathlon in the heat. J Sports Sci 32: 1680–1687. 10.1080/02640414.2014.915425
- Jeukendrup AE (2011) Nutrition for endurance sports: marathon, triathlon, and road cycling. J Sports Sci 29 Suppl 1: S91–99. 10.1080/02640414.2011.610348
- Garcia-Manso JM, Rodriguez-Ruiz D, Rodriguez-Matoso D, de Saa Y, Sarmiento S, Quiroga M (2011) Assessment of muscle fatigue after an ultra-endurance triathlon using tensiomyography (TMG). J Sports Sci 29: 619–625. 10.1080/02640414.2010.548822
- Del Coso J, Areces F, Salinero JJ, Gonzalez-Millan C, Abian-Vicen J, Soriano L, et al. (2014) Compression stockings do not improve muscular performance during a half-ironman triathlon race. Eur J Appl Physiol 114: 587–595. 10.1007/s00421-013-2789-2
- Del Coso J, Gonzalez-Millan C, Salinero JJ, Abian-Vicen J, Soriano L, Garde S, et al. (2012) Muscle damage and its relationship with muscle fatigue during a half-iron triathlon. PLoS One 7: e43280 10.1371/journal.pone.0043280
- Del Coso J, Fernandez de Velasco D, Abian-Vicen J, Salinero JJ, Gonzalez-Millan C, Areces F, et al. (2013) Running pace decrease during a marathon is positively related to blood markers of muscle damage. PLoS One 8: e57602 10.1371/journal.pone.0057602
- Del Coso J, Salinero JJ, Abian-Vicen J, Gonzalez-Millan C, Garde S, Vega P, et al. (2013) Influence of body mass loss and myoglobinuria on the development of muscle fatigue after a marathon in a warm environment. Appl Physiol Nutr Metab 38: 286–291. 10.1139/apnm-2012-0241
- Roelands B, de Koning J, Foster C, Hettinga F, Meeusen R (2013) Neurophysiological determinants of theoretical concepts and mechanisms involved in pacing. Sports Med 43: 301–311. 10.1007/s40279-013-0030-4
- Maughan RJ, Shirreffs SM (2012) Nutrition for sports performance: issues and opportunities. Proc Nutr Soc 71: 112–119. 10.1017/S0029665111003211
- Anthony JC, Yoshizawa F, Anthony TG, Vary TC, Jefferson LS, Kimball SR (2000) Leucine stimulates translation initiation in skeletal muscle of postabsorptive rats via a rapamycin-sensitive pathway. J Nutr 130: 2413–2419.
- Tang FC (2006) Influence of branched-chain amino acid supplementation on urinary protein metabolite concentrations after swimming. J Am Coll Nutr 25: 188–194.
- Lynch CJ, Halle B, Fujii H, Vary TC, Wallin R, Damuni Z, et al. (2003) Potential role of leucine metabolism in the leucine-signaling pathway involving mTOR. Am J Physiol Endocrinol Metab 285: E854–863.
- Greer BK, White JP, Arguello EM, Haymes EM (2011) Branched-chain amino acid supplementation lowers perceived exertion but does not affect performance in untrained males. J Strength Cond Res 25: 539–544. 10.1519/JSC.0b013e3181bf443a
- Nosaka K, Sacco P, Mawatari K (2006) Effects of amino acid supplementation on muscle soreness and damage. Int J Sport Nutr Exerc Metab 16: 620–635.
- Shimomura Y, Yamamoto Y, Bajotto G, Sato J, Murakami T, Shimomura N, et al. (2006) Nutraceutical effects of branched-chain amino acids on skeletal muscle. J Nutr 136: 529S–532S.
- Howatson G, Hoad M, Goodall S, Tallent J, Bell PG, French DN (2012) Exercise-induced muscle damage is reduced in resistance-trained males by branched chain amino acids: a randomized, double-blind, placebo controlled study. J Int Soc Sports Nutr 9: 20 10.1186/1550-2783-9-20
- Greer BK, Woodard JL, White JP, Arguello EM, Haymes EM (2007) Branched-chain amino acid supplementation and indicators of muscle damage after endurance exercise. Int J Sport Nutr Exerc Metab 17: 595–607.
- Coombes JS, McNaughton LR (2000) Effects of branched-chain amino acid supplementation on serum creatine kinase and lactate dehydrogenase after prolonged exercise. J Sports Med Phys Fitness 40: 240–246.
- Koba T, Hamada K, Sakurai M, Matsumoto K, Hayase H, Imaizumi K, et al. (2007) Branched-chain amino acids supplementation attenuates the accumulation of blood lactate dehydrogenase during distance running. J Sports Med Phys Fitness 47: 316–322.
- Knechtle B, Knechtle P, Mrazek C, Senn O, Rosemann T, Imoberdorf R, et al. (2011) No effect of short-term amino acid supplementation on variables related to skeletal muscle damage in 100 km ultra-runners—a randomized controlled trial. J Int Soc Sports Nutr 8: 6 10.1186/1550-2783-8-6
- Areces F, Salinero JJ, Abian-Vicen J, González-Millán C, Gallo-Salazar C, Ruiz-Vicente D, et al. (2014) A 7-day oral supplementation with branched-chain amino acids was ineffective to prevent muscle damage during a marathon. Amino Acids 46: 1169–1176. 10.1007/s00726-014-1677-3
- Chang CK, Chang Chien KM, Chang JH, Huang MH, Liang YC, Liu TH (2015) Branched-chain amino acids and arginine improve performance in two consecutive days of simulated handball games in male and female athletes: a randomized trial. PLoS One 10: e0121866 10.1371/journal.pone.0121866
- Hsu MC, Chien KY, Hsu CC, Chung CJ, Chan KH, Su B (2011) Effects of BCAA, arginine and carbohydrate combined drink on post-exercise biochemical response and psychological condition. Chin J Physiol 54: 71–78.
- Matsumoto K, Koba T, Hamada K, Tsujimoto H, Mitsuzono R (2009) Branched-chain amino acid supplementation increases the lactate threshold during an incremental exercise test in trained individuals. J Nutr Sci Vitaminol (Tokyo) 55: 52–58.
- Newsholme EA, Blomstrand E (2006) Branched-chain amino acids and central fatigue. J Nutr 136: 274S–276S.
- Cheuvront SN, Carter R 3rd, Kolka MA, Lieberman HR, Kellogg MD, Sawka MN (2004) Branched-chain amino acid supplementation and human performance when hypohydrated in the heat. J Appl Physiol (1985) 97: 1275–1282.
- Meeusen R, Watson P (2007) Amino acids and the brain: do they play a role in "central fatigue"? Int J Sport Nutr Exerc Metab 17 Suppl: S37–46.
- Fernstrom JD, Fernstrom MH (2006) Exercise, serum free tryptophan, and central fatigue. J Nutr 136: 553S–559S.
- Blomstrand E, Celsing F, Newsholme EA (1988) Changes in plasma concentrations of aromatic and branched-chain amino acids during sustained exercise in man and their possible role in fatigue. Acta Physiol Scand 133: 115–121.
- Smriga M, Kameishi M, Torii K (2006) Exercise-dependent preference for a mixture of branched-chain amino acids and homeostatic control of brain serotonin in exercising rats. J Nutr 136: 548S–552S.
- Assenza A, Bergero D, Tarantola M, Piccione G, Caola G (2004) Blood serum branched chain amino acids and tryptophan modifications in horses competing in long-distance rides of different length. J Anim Physiol Anim Nutr (Berl) 88: 172–177.
- Nelson AR, Phillips SM, Stellingwerff T, Rezzi S, Bruce SJ, Breton I, et al. (2012) A protein-leucine supplement increases branched-chain amino acid and nitrogen turnover but not performance. Med Sci Sports Exerc 44: 57–68. 10.1249/MSS.0b013e3182290371
- Crowe MJ, Weatherson JN, Bowden BF (2006) Effects of dietary leucine supplementation on exercise performance. Eur J Appl Physiol 97: 664–672.
- Cooper C, Packer N, Williams K (2001) Amino acid analysis protocols Totowa, NJ: Springer Science & Business Media.
- Burger-Mendonca M, Bielavsky M, Barbosa FC (2008) Liver overload in Brazilian triathletes after half-ironman competition is related muscle fatigue. Ann Hepatol 7: 245–248.
- Suzuki K, Peake J, Nosaka K, Okutsu M, Abbiss CR, Surriano R, et al. (2006) Changes in markers of muscle damage, inflammation and HSP70 after an Ironman Triathlon race. Eur J Appl Physiol 98: 525–534.
- Stepanyan V, Crowe M, Haleagrahara N, Bowden B (2014) Effects of vitamin E supplementation on exercise-induced oxidative stress: a meta-analysis. Appl Physiol Nutr Metab 39: 1029–1037. 10.1139/apnm-2013-0566
- Margaritis I, Tessier F, Verdera F, Bermon S, Marconnet P (1999) Muscle enzyme release does not predict muscle function impairment after triathlon. J Sports Med Phys Fitness 39: 133–139.
- Dohm GL, Tapscott EB, Kasperek GJ (1987) Protein degradation during endurance exercise and recovery. Med Sci Sports Exerc 19: S166–171.
- Mueller SM, Anliker E, Knechtle P, Knechtle B, Toigo M (2013) Changes in body composition in triathletes during an Ironman race. Eur J Appl Physiol 113: 2343–2352. 10.1007/s00421-013-2670-3
- Blomstrand E (2006) A role for branched-chain amino acids in reducing central fatigue. J Nutr 136: 544S–547S.
- Chaouloff F, Laude D, Elghozi JL (1989) Physical exercise: evidence for differential consequences of tryptophan on 5-HT synthesis and metabolism in central serotonergic cell bodies and terminals. J Neural Transm 78: 121–130.
- Gleeson M (2005) Interrelationship between physical activity and branched-chain amino acids. J Nutr 135: 1591S–1595S.
- Varnier M, Sarto P, Martines D, Lora L, Carmignoto F, Leese GP, et al. (1994) Effect of infusing branched-chain amino acid during incremental exercise with reduced muscle glycogen content. Eur J Appl Physiol Occup Physiol 69: 26–31.
- van Hall G, Raaymakers JS, Saris WH, Wagenmakers AJ (1995) Ingestion of branched-chain amino acids and tryptophan during sustained exercise in man: failure to affect performance. J Physiol 486 (Pt 3): 789–794.
Source: PubMed