Effects of water extracts of Graptopetalum paraguayense on blood pressure, fasting glucose, and lipid profiles of subjects with metabolic syndrome

Chi-Hua Yen, Shu-Ju Chen, Jen-Tzu Liu, Yu-Fen Tseng, Ping-Ting Lin, Chi-Hua Yen, Shu-Ju Chen, Jen-Tzu Liu, Yu-Fen Tseng, Ping-Ting Lin

Abstract

This study was aimed to investigate the effects of water extracts of Graptopetalum paraguayense (WGP, 4 g/d) on blood pressure, blood glucose level, and lipid profiles in subjects with metabolic syndrome (MS). Participants with MS (n = 54) were randomly assigned to the placebo (n = 28) and WGP groups (n = 26), and the intervention was administered for 12 weeks. Systolic blood pressure (SBP), diastolic blood pressure (DBP), fasting glucose (FG), lipid profiles (total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), and high density lipoprotein (HDL-C)), and antioxidant enzymes activities (catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx)) were measured. Forty-two subjects completed the study (placebo, n = 19; WGP, n = 23). FG, SBP, and LDL-C levels were significantly lower and HDL-C level and antioxidant enzymes activities (CAT and SOD) were significantly higher after WGP supplementation. Blood pressure, FG, and lipid profiles were significantly correlated with antioxidant enzymes activities after supplementation (P < 0.05). The present study demonstrated a significant reduction in blood pressure, blood glucose, and lipid profiles and an increase in antioxidant enzymes activities in subjects with MS after WGP supplementation. Taken together, the antioxidative capacity of WGP might exert a beneficial effect on MS. This trial is registered with ClinicalTrials.gov NCT01463748.

Figures

Figure 1
Figure 1
CONSORT scheme. WGP: water extracts of Graptopetalum paraguayense.
Figure 2
Figure 2
Blood pressure measurements. Data are means ± SD. □ Week 0, ■ Week 12. *Values were significantly different after intervention within the group, P < 0.05. a,bValues with different superscripts were significantly different between two groups, P < 0.05. DBP: diastolic blood pressure; SBP: systolic blood pressure; WGP: water extracts of Graptopetalum paraguayense.
Figure 3
Figure 3
Fasting glucose level and blood lipid profiles. Data are means ± SD. □ Week 0, ■ Week 12. *Values were significantly different after intervention within the group; *P = 0.06; **P = 0.01. a,bValues with different superscripts were significantly different between two groups, P < 0.05. FG: fasting glucose; HDL-C: high density lipoprotein cholesterol; LDL-C: low density lipoprotein cholesterol; TC: total cholesterol; TG: triglyceride; WGP: water extracts of Graptopetalum paraguayense.
Figure 4
Figure 4
Antioxidant enzymes activities. Data are means ± SD. □ Week 0, ■ Week 12. *Values were significantly different after intervention within the group, P < 0.05. a, bValues with different superscripts were significantly different between two groups, P < 0.05. CAT: catalase; GPx: glutathione peroxidase; SOD: superoxide dismutase; WGP: water extracts of Graptopetalum paraguayense.

References

    1. Cleeman JI. Executive summary of the third report of the National Cholesterol Education Program (NCEP) expert panel on detection, evaluation, and treatment of high blood cholesterol in adults (adult treatment panel III) The Journal of the American Medical Association. 2001;285(19):2486–2497.
    1. Ferrannini E, Haffner SM, Mitchell BD, Stern MP. Hyperinsulinaemia: the key feature of cardiovascular and metabolic syndrome. Diabetologia. 1991;34(6):416–422.
    1. Ford ES, Giles WH. A comparison of the prevalence of the metabolic syndrome using two proposed definitions. Diabetes Care. 2003;26(3):575–581.
    1. Park Y-W, Zhu S, Palaniappan L, Heshka S, Carnethon MR, Heymsfield SB. The metabolic syndrome: prevalence and associated risk factor findings in the US population from the Third National Health and Nutrition Examination Survey, 1988–1994. Archives of Internal Medicine. 2003;163(4):427–436.
    1. Kolovou GD, Anagnostopoulou KK, Salpea KD, Mikhailidis DP. The prevalence of metabolic syndrome in various populations. The American Journal of the Medical Sciences. 2007;333(6):362–371.
    1. Yeh C-J, Chang H-Y, Pan W-H. Time trend of obesity, the metabolic syndrome and related dietary pattern in Taiwan: from NAHSIT 1993-1996 to NAHSIT 2005-2008. Asia Pacific Journal of Clinical Nutrition. 2011;20(2):292–300.
    1. Ceriello A, Quatraro A, Giugliano D. Diabetes mellitus and hypertension: the possible role of hyperglycaemia through oxidative stress. Diabetologia. 1993;36(3):265–266.
    1. Giugliano D, Ceriello A, Paolisso G. Diabetes mellitus, hypertension, and cardiovascular disease: which role for oxidative stress? Metabolism. 1995;44(3):363–368.
    1. West IC. Radicals and oxidative stress in diabetes. Diabetic Medicine. 2000;17(3):171–180.
    1. Antoniades C, Tousoulis D, Tentolouris C, Toutouzas P, Stefanadis C. Oxidative stress, antioxidant vitamins, and atherosclerosis: from basic research to clinical practice. Herz. 2003;28(7):628–638.
    1. Stocker R, Keaney JF., Jr. Role of oxidative modifications in atherosclerosis. Physiological Reviews. 2004;84(4):1381–1478.
    1. Penckofer S, Schwertz D, Florczak K. Oxidative stress and cardiovascular disease in type 2 diabetes: the role of antioxidants and pro-oxidants. The Journal of Cardiovascular Nursing. 2002;16(2):68–85.
    1. Giugliano D. Dietary antioxidants for cardiovascular prevention. Nutrition, Metabolism and Cardiovascular Diseases. 2000;10(1):38–44.
    1. Huang K-F, Chen Y-W, Chang C-T, Chou S-T. Studies on the inhibitory effect of Graptopetalum paraguayense E. Walther extracts on mushroom tyrosinase. Food Chemistry. 2005;89(4):583–587.
    1. Chung Y-C, Chen S-J, Hsu C-K, Chang C-T, Chou S-T. Studies on the antioxidative activity of Graptopetalum paraguayense E. Walther. Food Chemistry. 2005;91(3):419–424.
    1. Chen S-J, Chang C-T, Chung Y-C, Chou S-T. Studies on the inhibitory effect of Graptopetalum paraguayense E. Walther extracts on the angiotensin converting enzyme. Food Chemistry. 2007;100(3):1032–1036.
    1. Chung Y-C, Chen S-J, Pengspi-Sup H-Y, Chou S-T. Anti hypertensive and antioxidant effects of the Graptopetalum paraguayense E. Walther extract in spontaneously hypertensive rats. Journal of the Science of Food and Agriculture. 2009;89(15):2678–2686.
    1. Chen S-J, Chung J-G, Chung Y-C, Chou S-T. In vitro antioxidant and antiproliferative activity of the stem extracts from Graptopetalum paraguayense . American Journal of Chinese Medicine. 2008;36(2):369–383.
    1. Duh P-D, Lin S-L, Wu S-C. Hepatoprotection of Graptopetalum paraguayense E. Walther on CCl 4-induced liver damage and inflammation. Journal of Ethnopharmacology. 2011;134(2):379–385.
    1. Su LJ, Chang CC, Yang CH, et al. Graptopetalum paraguayense ameliorates chemical-induces rat hepatic fibrosis in vivo and inactivates stellate cells and kupffer cells in vitro. PLoS ONE. 2013;(8)e53988
    1. Kao T-K, Ou Y-C, Raung S-L, et al. Graptopetalum paraguayense E. Walther leaf extracts protect against brain injury in ischemic rats. American Journal of Chinese Medicine. 2010;38(3):495–516.
    1. Lin YL, Peng HY, Hsieh HM, Lin CH, Chou ST. Effects of Graptopetalum paraguayense consumption on serum lipid profiles and antioxidative status in hypercholesteremic subjects. Journal of the Science of Food and Agriculture. 2011;91(7):1230–1235.
    1. Alberti KGMM, Zimmet P. The metabolic syndrome—a new worldwide definition. The Lancet. 2005;366(9491):1059–1062.
    1. Grundy SM, Cleeman JI, Daniels SR, et al. Diagnosis and management of the metabolic syndrome: an American Heart Association/National Heart, Lung, and Blood Institute scientific statement. Circulation. 2005;112(17):2735–2752.
    1. Chung Y-C, Chou S-T, Jhan J-K, Liao J-W, Chen S-J. In vitro and in vivo safety of aqueous extracts of Graptopetalum paraguayense E. Walther. Journal of Ethnopharmacology. 2012;140(1):91–97.
    1. Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. The Journal of Laboratory and Clinical Medicine. 1967;70(1):158–169.
    1. Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemistry. 1974;47(3):469–474.
    1. Aebi H. [13] Catalase in vitro. Methods in Enzymology. 1984;105(C):121–126.
    1. Karaouzene N, Merzouk H, Aribi M, et al. Effects of the association of aging and obesity on lipids, lipoproteins and oxidative stress biomarkers: a comparison of older with young men. Nutrition, Metabolism and Cardiovascular Diseases. 2011;21(10):792–799.
    1. Van Acker SABE, Van Den Berg D-J, Tromp MNJL, et al. Structural aspects of antioxidant activity of flavonoids. Free Radical Biology & Medicine. 1996;20(3):331–342.
    1. Zheng W, Wang SY. Antioxidant activity and phenolic compounds in selected herbs. Journal of Agricultural and Food Chemistry. 2001;49(11):5165–5170.
    1. Evans JL, Goldfine ID, Maddux BA, Grodsky GM. Are oxidative stress—activated signaling pathways mediators of insulin resistance and β-cell dysfunction? Diabetes. 2003;52(1):1–8.
    1. Yang JI, Pan MH, Tsai ML, Chen WC. Effects of Shi-lan (Graptopetalum paraguayens) extracts on antioxidative performance and serum glucose level in Streptozotocin-induced diabetic rats. Journal of National Kaohsiung Marine University. 2003;18:119–132.
    1. Poitout V, Robertson RP. Minireview: secondary β-cell failure in type 2 diabetes—a convergence of glucotoxicity and lipotoxicity. Endocrinology. 2002;143(2):339–342.
    1. Blatter M-C, James RW, Messner S, Barja F, Pometta D. Identification of a distinct human high-density lipoprotein subspecies defined by a lipoprotein-associated protein, K-45. Identity of K-45 with paraoxonase. European Journal of Biochemistry. 1993;211(3):871–879.
    1. Rao MN, Liu Q-H, Marmillot P, Seeff LB, Strader DB, Lakshman MR. High-density lipoproteins from human alcoholics exhibit impaired reverse cholesterol transport function. Metabolism. 2000;49(11):1406–1410.
    1. Osaki F, Ikeda Y, Suehiro T, et al. Roles of Sp1 and protein kinase C in regulation of human serum paraoxonase 1 (PON1) gene transcription in HepG2 cells. Atherosclerosis. 2004;176(2):279–287.
    1. Cheng C-C, Wu L-C, Lai J-M, Chen C-T, Hsueh C-M, Hsu S-L. Ethanol extract of Graptopetalum paraguayense upregulates paraoxonase 1 gene expression via an AKT/NF-κB-dependent pathway. American Journal of Chinese Medicine. 2012;40(2):357–372.
    1. Mackness B, Durrington P, Povey A, et al. Paraoxonase and susceptibility to organophosphorus poisoning in farmers dipping sheep. Pharmacogenetics. 2003;13(2):81–88.
    1. Papathanasopoulos A, Camilleri M. Dietary fiber supplements: effects in obesity and metabolic syndrome and relationship to gastrointestinal functions. Gastroenterology. 2010;138(1):65–e2.
    1. Assini JM, Mulvihill EE, Huff MW. Citrus flavonoids and lipid metabolism. Current Opinion in Lipidology. 2013;24(1):34–40.

Source: PubMed

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