Phenotypic Characterization of Alveolar Macrophages 2016 (Caract-Aires-2016) (Caract-Aires)

Phenotypic Characterization of Alveolar Macrophages 2016

The aim of the study is to investigate by flow cytometry the phenotype of alveolar macrophages (AMs) isolated from patients with non-infectious lung diseases.

Study Overview

• Status: Unknown
• Conditions: Lung Diseases

Detailed Description

Although alveolar macrophages (AMs) represent the main immune cell population in the human lungs, consensus on their phenotypic characterization is still missing.

Current translational protocols use human AMs obtained by invasive methods (broncho-alveolar lavage and lung biopsy) or differentiate macrophages from blood monocytes cultured in the presence of growth factors such as macrophage colony-stimulating factor (M-CSF) or granulocyte-macrophage colony-stimulating factor (GM-CSF). The group has already published the phenotypic and functional comparison of human blood monocyte-derived macrophages differentiated with M-CSF or GM-CSF. Aim of this study is to use a multi-staining flow cytometry (FACS analysis) to better characterize the phenotype of AMs isolated from broncho-alveolar lavage fluids of patients affected by non-infectious lung diseases. The FACS profiles of AMs will be further compared to the phenotypic profile of blood monocyte-derived macrophages differentiated in the presence of GM-CSF or M-CSF. The results of this study will allow to define the most valid in vitro model of AMs in a non-infectious setting.

• Study Type: Observational
• Enrollment (Anticipated): 18

Contacts and Locations

Study Locations

• France
  • Rennes, France, 35033
    • Recruiting
    • Pneumology Division
    • Contact: Stéphane Jouneau, Professor; Phone Number: +33 2 99 28 24 81; Email: stephane.jouneau@chu-rennes.fr

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years and older (Adult, Older Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

• Sampling Method: Non-Probability Sample

Study Population

Patients affected by non-infectious lung diseases older than 18 years

Description

Inclusion Criteria:

• Patients affected by non-infectious lung diseases and for whom a broncho-alveolar lavage is scheduled for their medical care in the pulmonary division
• Patient Information leaflet provided
• Written informed consent of the patient

Exclusion Criteria:

• A suspected respiratory infection

Study Plan

How is the study designed?

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
To investigate by flow cytometry the phenotype of alveolar macrophages (AMs) isolated from broncho-alveolar lavage fluids of patients with non-infectious lung diseases.	Characterization of membrane proteins by multi-staining flow cytometry (FACS analysis)	1 year

Secondary Outcome Measures

Outcome Measure	Time Frame
To compare the phenotypic profile of AMs from broncho-alveolar lavage fluids of patients with non-infectious lung diseases to that of blood monocyte-derived macrophages differentiated in the presence of GM-CSF or M-CSF and obtained from healthy donors	1 year

Collaborators and Investigators

• Sponsor: Rennes University Hospital
• Investigators: Principal Investigator: Stéphane Jouneau, Professor, Rennes University Hospital

Study record dates

Study Major Dates

• Study Start (Actual): November 1, 2016
• Primary Completion (Anticipated): December 1, 2017
• Study Completion (Anticipated): September 1, 2018

Study Registration Dates

• First Submitted: August 11, 2017
• First Submitted That Met QC Criteria: August 11, 2017
• First Posted (Actual): August 16, 2017

Study Record Updates

• Last Update Posted (Actual): August 16, 2017
• Last Update Submitted That Met QC Criteria: August 11, 2017
• Last Verified: August 1, 2017

More Information

Terms related to this study

• Keywords: Alveolar macrophages; Blood monocyte-derived macrophages; FACS analysis; Broncho-alveolar lavage fluids; Non-infectious lung diseases
• Additional Relevant MeSH Terms: Respiratory Tract Diseases; Lung Diseases
• Other Study ID Numbers: 35RC16_3055_Caract-Aires-2016

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No