Effect of Exercise on Muscle Proteome Signature

Effect of Exercise Training on Skeletal Muscle Proteome Signature of Young Men

The aim of the study is to investigate skeletal muscle proteome signature changes induced by exercise training in young men

Study Overview

• Status: Completed
• Conditions: Healthy
• Intervention / Treatment: Other: exercise training

Detailed Description

Advances in omics technologies have allowed for the identification of biomarkers with importance for the beneficial adaptations imposed by exercise training. Skeletal muscle exhibits remarkable plasticity to exercise training, as illustrated by pronounced alterations to the proteomic landscape, such as mitochondrial biogenesis. However, despite recent advances that have helped describe the proteomic landscape in response to a period of exercise training, our understanding of the processes pertaining to post-translation modification is scant. In this study, we aim to investigate the human acetylome and proteome in response to exercise training.

• Study Type: Interventional
• Enrollment (Actual): 8
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Denmark
  • Copenhagen, Denmark, 2100
    • Department of Nutrition, Exercise and Sports

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 40 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Male

Description

Inclusion Criteria:

• Healthy
• VO2max of 45-55 ml/kg/min (+/- SD) / 3000-4000 ml/min (+/- SD)
• Body mass index (BMI) of 19-26 kg/m2
• Normal ECG (electrocardiography)

Exclusion Criteria:

• Serious adverse effects to exercise
• Chronic disease

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: N/A
• Interventional Model: Single Group Assignment
• Masking: None (Open Label)

Number of Arms

1

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Exercise; 12-15 training sessions of 30 min over 4-5 weeks	Other: exercise training; subjects complete 4-5 weeks of training

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Skeletal muscle proteome signature	proteomics	Change from baseline proteome signature at 5 weeks

Other Outcome Measures

Outcome Measure	Measure Description	Time Frame
Skeletal muscle acetylome signature	acetylomics	Change from baseline acetylome signature at 5 weeks
Skeletal muscle mitochondrial respiration	Mitochondrial respiratory capacity	Change from baseline at 5 weeks
Maximal oxygen uptake	Maximal oxygen uptake	Change from baseline at 5 weeks

Collaborators and Investigators

• Sponsor: Morten Hostrup, PhD

Study record dates

Study Major Dates

• Study Start (Actual): August 28, 2017
• Primary Completion (Actual): October 15, 2017
• Study Completion (Actual): October 15, 2017

Study Registration Dates

• First Submitted: August 28, 2017
• First Submitted That Met QC Criteria: August 29, 2017
• First Posted (Actual): September 1, 2017

Study Record Updates

• Last Update Posted (Actual): January 15, 2021
• Last Update Submitted That Met QC Criteria: January 12, 2021
• Last Verified: January 1, 2021

More Information

Terms related to this study

• Other Study ID Numbers: ACE

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: UNDECIDED

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No