Fasting and Exercise-induced Changes in Human Skeletal Muscle

Impact of Exercise-induced Energetic Stress During an 8-hour Fast on Metabolic Regulation in Resting Human Skeletal Muscle

This study examines the impact of increasing energetic stress during an 8-hour fast on metabolic pathways in human skeletal muscle. Specifically, participants will undergo two 8-hour fasting sessions in a randomized crossover fashion, with the addition of low-intensity arm exercise in one of the sessions. Changes in metabolic pathways will be examined in resting (leg) muscle before, during, and after the fast.

Study Overview

• Status: Completed
• Conditions: Skeletal Muscle Energetics
• Intervention / Treatment: Other: FAST+EX; Other: FAST

• Study Type: Interventional
• Enrollment (Actual): 10
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Canada
  • Ontario
    • Kingston, Ontario, Canada, K7L3N6
      • School of Kinesiology and Health Studies

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 30 years (Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: Male

Description

Inclusion Criteria:

• recreationally active (no more than 3 hours of weekly physical activity)
• not currently involved in a systematic training program
• body mass index < 30 kg/m2

Exclusion Criteria:

• presence of cardiometabolic disease
• taking regular oral medication
• current smoker

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: Double

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: FAST+EX - FAST; FAST+EX then FAST alone	Other: FAST+EX; Participants will perform 2-hours of low-intensity arm exercise (8 x 15 min bouts of arm ergometer exercise separated by 10 min of rest) during an 8-hour fast.; Other: FAST; Participants will complete an 8-hour fast.
Experimental: FAST - FAST+EX; FAST alone then FAST+EX	Other: FAST+EX; Participants will perform 2-hours of low-intensity arm exercise (8 x 15 min bouts of arm ergometer exercise separated by 10 min of rest) during an 8-hour fast.; Other: FAST; Participants will complete an 8-hour fast.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Peroxisome proliferator activated receptor gamma co-activator 1-alpha (PGC-1α) messenger ribonucleic acid (mRNA) expression	PGC-1α gene expression (indirect indicator of PGC-1α activity)	15 min before fast; after 4 and 8 hours of fasting

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Catecholamine concentrations	Epinephrine and norepinephrine concentrations in plasma samples	15 min before fast; after exercise; after 4 and 8 hours of fasting
Glucose and insulin concentrations	Glucose and insulin concentrations in plasma samples	15 min before fast; after 4 and 8 hours of fasting
total and phosphorylated AMP activated protein kinase (AMPK)	active AMPK relative to total AMPK protein content	15 min before fast; after 4 and 8 hours of fasting
total and phosphorylated SIRT-1	active SIRT-1 relative to total SIRT1 protein content	15 min before fast; after 4 and 8 hours of fasting
total and phosphorylated mammalian target of rapamycin (mTOR)	active mTOR relative to total mTOR protein content	15 min before fast; after 4 and 8 hours of fasting
total and phosphorylated Akt/Protein Kinase B	active Akt relative to total Akt protein content	15 min before fast; after 4 and 8 hours of fasting
total and phosphorylated acetyl coenzyme A carboxylase (ACC)	active ACC relative to total ACC protein	15 min before fast; after 4 and 8 hours of fasting
total and phosphorylated p38 mitogen activated protein kinase (MAPK)	active p38 MAPK relative to total p38 MAPK protein	15 min before fast; after 4 and 8 hours of fasting
phosphorylated protein kinase A (PKA) substrates	phosphorylated PKA substrates (indirect measure of PKA activity)	15 min before fast; after 4 and 8 hours of fasting
acetylated p53	p53 acetylation status (indirect measure of SIRT-1 deacetylase activity)	15 min before fast; after 4 and 8 hours of fasting
Sirtuin 1 (SIRT1) mRNA expression	gene expression of the deacetylase enzyme SIRT1	15 min before fast; after 4 and 8 hours of fasting
Sirtuin 3 (SIRT3) mRNA expression	gene expression of the deacetylase enzyme SIRT3	15 min before fast; after 4 and 8 hours of fasting
Pyruvate dehydrogenase kinase isoenzyme 4 (PDK4) mRNA expression	gene expression of the PGC-1α target PDK4	15 min before fast; after 4 and 8 hours of fasting
p53 mRNA expression	gene expression of the tumour suppressor protein p53	15 min before fast; after 4 and 8 hours of fasting
Leucine rich pentatricopeptide repeat containing protein (LRP130) mRNA expression	gene expression of RNA-binding protein LRP130	15 min before fast; after 4 and 8 hours of fasting
General control non-repressible 5 (GCN5) mRNA expression	gene expression of the acetyltransferase GCN5	15 min before fast; after 4 and 8 hours of fasting
PGC-1α protein content	Protein content of the transcriptional co-activator PGC-1α	15 min before fast; after 4 and 8 hours of fasting
Cytochrome C oxidase subunit 4 (COX-IV) protein content	Protein content of the mitochondrial enzyme COX subunit 4	15 min before fast; after 4 and 8 hours of fasting
Intramuscular glycogen content	Muscle glycogen content	15 min before fast; after 4 and 8 hours of fasting
Intramuscular triglyceride (IMTG) content	Muscle IMTG content	15 min before fast; after 4 and 8 hours of fasting

Collaborators and Investigators

• Sponsor: Queen's University
• Collaborators: University of Waterloo
• Investigators: Principal Investigator: Brendon J. Gurd, PhD, Queen's University

Study record dates

Study Major Dates

• Study Start (Actual): January 14, 2019
• Primary Completion (Actual): April 30, 2019
• Study Completion (Actual): April 30, 2019

Study Registration Dates

• First Submitted: January 17, 2019
• First Submitted That Met QC Criteria: January 17, 2019
• First Posted (Actual): January 22, 2019

Study Record Updates

• Last Update Posted (Actual): May 30, 2019
• Last Update Submitted That Met QC Criteria: May 29, 2019
• Last Verified: May 1, 2019

More Information

Terms related to this study

• Other Study ID Numbers: QMPL_HI-01-19

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: No

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No