Eccentric Cycling Exercise on Mitochondrial Function of Lymphocyte

August 27, 2024 updated by: Jong-Shyan Wang, Chang Gung Memorial Hospital

Effects of Concentric and Eccentric Exercise Regimens on Bioenergetic Efficiency of Lymphocytes in Sedentary Males

Eccentric cycling exercise (ECE) features lower metabolic demand and higher improvement of muscle strength compared to traditional concentric cycling exercise (CCE). Mitochondria can regulate energy metabolism and adaptive immune quality in T lymphocytes. However, the effects of ECE on mitochondrial functions in T-lymphocytes have not yet been established.

Method: A total of 33 healthy sedentary males were randomized and divided into ECE (n=11), CCE (n=11), and control groups (n=11). These subjects progressively performed CCE or ECE from 60% to 80% maximal workload on a bicycle ergometer for 40 min/day, 5 days/week for 6 weeks. A graded exercise testing and an isokinetic strength test were conducted to evaluate cardiopulmonary fitness and muscle strength, respectively. Phenotypes and mitochondrial respiratory capacity in T lymphocyte were analyzed using flow cytometry and high-resolution respirometer, respectively.

Study Overview

Status

Completed

Conditions

Intervention / Treatment

Detailed Description

Eccentric exercise training (EET) increases physical performance while having lower metabolic demand than concentric exercise training (CET).

Mitochondria of lymphocytes are essential for cell proliferation, death, and differentiation, and play a critical role in establishing lymphocyte phenotypes and their functions.

Whether EET influences lymphocyte bioenergetic efficiency remains unclear. The study was to investigate the effects of ECE and CCE regimens on adaptive immune functions and mitochondrial bioenergetics of T lymphocytes in sedentary males.

Study Type

Interventional

Enrollment (Actual)

33

Phase

  • Not Applicable

Contacts and Locations

This section provides the contact details for those conducting the study, and information on where this study is being conducted.

Study Locations

  • Taiwan
      • Taoyuan, Taiwan, 333
        • Chang Gung University

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

Eligibility Criteria

Ages Eligible for Study

  • Adult

Accepts Healthy Volunteers

Yes

Description

Inclusion Criteria:

  1. Sedentary lifestyle.
  2. Nonsmokers, nonusers of medications/vitamins.
  3. No any cardiopulmonary/hematological risks.

Exclusion Criteria:

  1. Have regular exercise habits (i.e., exercise frequency once per week, duration >20 min).
  2. Have retabolic or musculoskeletal disease.

Study Plan

This section provides details of the study plan, including how the study is designed and what the study is measuring.

How is the study designed?

Design Details

  • Primary Purpose: Treatment
  • Allocation: Randomized
  • Interventional Model: Parallel Assignment
  • Masking: Single

Arms and Interventions

Participant Group / Arm
Intervention / Treatment
Experimental: Eccentric cycling training
Moderate to high intensity cycling training in eccentric type
Behavioral: Eccentric cycling training

Performed exercise training 5 days a week for 6 weeks on an eccentric ergometer.

Each training session: 5 min at 30% of maximal workload (Wmax) for warmed up and cold down and 30 min the main training phase.

Intensity: Firstly, set at 60% Wmax, and progressively increased 5% per week
Experimental: Concentric cycling training
Moderate to high intensity cycling training in concentric type (intensity matched the eccentric training)
Behavioral: Concentric cycling training

Performed exercise training 5 days a week for 6 weeks on an eccentric ergometer.

Each training session: 5 min at 30% of maximal workload (Wmax) for warmed up and cold down and 30 min the main training phase.

Intensity: Firstly, set at 60% Wmax, and progressively increased 5% per week
No Intervention: Control group
without receiving any exercise training

What is the study measuring?

Primary Outcome Measures

Outcome Measure
Measure Description
Time Frame
Mitochondrial function of lymphocyte
Time Frame: 12 weeks
Evaluate bioenergetic health index (BHI) of mitochondria in lymphocyte by using a high resolution respirometer.
12 weeks

Secondary Outcome Measures

Outcome Measure
Measure Description
Time Frame
Cardiopulmonary fitness
Time Frame: 12 weeks
The graded exercise test (GXT) on a bicycle ergometer was performed. The GXT comprised 2 min of unloaded pedaling followed by a continuous increase in the work rate of 30 watt per 3-minute until exhaustion (i.e., progressive exercise to maximal O2 consumption)
12 weeks

Collaborators and Investigators

This is where you will find people and organizations involved with this study.

Sponsor

Chang Gung Memorial Hospital

Publications and helpful links

The person responsible for entering information about the study voluntarily provides these publications. These may be about anything related to the study.

General Publications

Study record dates

These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.

Study Major Dates

Study Start (Actual)

January 1, 2021

Primary Completion (Actual)

January 1, 2022

Study Completion (Actual)

August 1, 2022

Study Registration Dates

First Submitted

August 20, 2024

First Submitted That Met QC Criteria

August 27, 2024

First Posted (Actual)

August 29, 2024

Study Record Updates

Last Update Posted (Actual)

August 29, 2024

Last Update Submitted That Met QC Criteria

August 27, 2024

Last Verified

August 1, 2024

More Information

Terms related to this study

Keywords

Other Study ID Numbers

  • 202100004A3

Plan for Individual participant data (IPD)

Plan to Share Individual Participant Data (IPD)?

NO

Drug and device information, study documents

Studies a U.S. FDA-regulated drug product

No

Studies a U.S. FDA-regulated device product

No

This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.

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