Eccentric Cycling Exercise With Assistive Devices on Mitochondrial Metabolism in T Lymphocyte

March 31, 2025 updated by: Chang Gung Memorial Hospital

Effects of Eccentric Cycling Exercise Training With Assistive Devices on Phenotypes and Mitochondrial Metabolism in T Lymphocytes in Sedentary Men

Eccentric cycling exercise can be a promising modality fwith lower respiratory demands and higher power output. Assistive devices like neuromuscular electrical stimulation (NMES) and compressive garment (CG) accelerates muscle pumping and relieve the eccentric exercise-induced muscle soreness.

Phenotypes and mitochondrial metabolism are crucial to regulate acquired immnunity of T lymphocytes. The study aimed to investigate the effects of eccentric cycling exercise training combined with NMES and CG on mitochondrial phenotypes and bioenergetics in T lymphocytes in sedentary males. The hypothesis were an acute/intense exercise decreased the mitochondrial respiratory capacities of OXPHOS, ETS and bioenergetics, while eccentric cycling training could elevate the diminished mitochondrial functions.

Methods: Twelve sedentary healthy males were randomly assigned to either eccentric cycling with assistive devices (ECA, n=10) or eccentric cycling only (ECC, n=10). All participants performed ECA or ECC at an intensity of 60% to 120% maximal workload progressively on a costumed bicycle ergometer for 40 min/day, 5 days/week for 5 weeks. ECA performed NMES for 20min/session after cycling, while ECC was seated for resting 20 min. Systemic aerobic fitness, phenotypes of T lymphocytes, mitochondrial OXPHOS, ETS respiratory capacities, and bioenergetics in T lymphocytes were analyzed.

Study Overview

Status

Completed

Conditions

Intervention / Treatment

Study Type

Interventional

Enrollment (Actual)

30

Phase

  • Not Applicable

Contacts and Locations

This section provides the contact details for those conducting the study, and information on where this study is being conducted.

Study Locations

  • Taiwan
    • Taoyuan
      • Guishan, Taoyuan, Taiwan, 333
        • Chang Gung University

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

Eligibility Criteria

Ages Eligible for Study

  • Adult

Accepts Healthy Volunteers

Yes

Description

Inclusion Criteria:

  • Sedentary lifestyle (exercise<20min/session; <1session/week for a year or longer).
  • Nonsmokers, nonusers of medications/vitamins.
  • No any cardiopulmonary, neuromuscular, and hematological risks.

Exclusion Criteria:

  • Have regular exercise habits (i.e., exercise frequency once per week, duration >20 min).
  • Have retabolic or musculoskeletal disease.

Study Plan

This section provides details of the study plan, including how the study is designed and what the study is measuring.

How is the study designed?

Design Details

  • Primary Purpose: Basic Science
  • Allocation: Randomized
  • Interventional Model: Parallel Assignment
  • Masking: None (Open Label)

Arms and Interventions

Participant Group / Arm
Intervention / Treatment
Experimental: Eccentric cycling exercise (ECC)
High intensity cycling training from 80% to 120% Wmax in eccentric type
Behavioral: Eccentric cycling exercise (ECC)

Performed exercise training 5 days a week for 5 weeks on an eccentric ergometer.

Each training session: 5 min at 30% of maximal workload (Wmax) for warmed up and cold down and 30 min the main training phase.

After each training session, rest in sitting position for 20 min. Exercise intensity: Firstly, set at 80% Wmax, and progressively increased 5% per week to 120 % Wmax in the last week.
Experimental: Eccentric cycling exercise with assistive devices (ECA)
High intensity cycling training from 80% to 120% Wmax in eccentric type (intensity matched the ECC)
Behavioral: Eccentric cycling exercise with assistive devices (ECA)

Performed exercise training 5 days a week for 5 weeks on an eccentric ergometer.

Each training session: 5 min at 30% of maximal workload (Wmax) for warmed up and cold down and 30 min the main training phase.

After each training session, receive NMES in sitting position for 20 min.

Intensity: Firstly, set at 80% Wmax, and progressively increased 5% per week to 120 % Wmax in the last week.
No Intervention: Control group
Perform no exercise training but maintain only regular daily lives only in the study.

What is the study measuring?

Primary Outcome Measures

Outcome Measure
Measure Description
Time Frame
Mitochondrial bioenergetics of oxygen consumption rate in T lymphocyte
Time Frame: At baseline and 2 days after completing the 5-week intervention
The bioenergetic health index (BHI) of mitochindria in T lymphocyte was measured. BHI is a parameter for detecting the health status of mitochondrial and efficiency of energy metabolism.
At baseline and 2 days after completing the 5-week intervention
Systemic aerobic fitness (VO2)
Time Frame: At baseline and 2 days after completing the 5-week intervention
VO2max was determined during the exercise testing. VO2max is the maximum of oxygen deliver and utilization.
At baseline and 2 days after completing the 5-week intervention

Collaborators and Investigators

This is where you will find people and organizations involved with this study.

Sponsor

Chang Gung Memorial Hospital

Publications and helpful links

The person responsible for entering information about the study voluntarily provides these publications. These may be about anything related to the study.

General Publications

Study record dates

These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.

Study Major Dates

Study Start (Actual)

February 23, 2023

Primary Completion (Actual)

February 23, 2024

Study Completion (Actual)

December 31, 2024

Study Registration Dates

First Submitted

January 8, 2025

First Submitted That Met QC Criteria

March 31, 2025

First Posted (Actual)

April 2, 2025

Study Record Updates

Last Update Posted (Actual)

April 2, 2025

Last Update Submitted That Met QC Criteria

March 31, 2025

Last Verified

January 1, 2025

More Information

Terms related to this study

Keywords

Other Study ID Numbers

  • 202202314A3

Plan for Individual participant data (IPD)

Plan to Share Individual Participant Data (IPD)?

NO

Drug and device information, study documents

Studies a U.S. FDA-regulated drug product

No

Studies a U.S. FDA-regulated device product

No

product manufactured in and exported from the U.S.

No

This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.

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