Comparison of Skin Condition After Hyaluronic Acid Application on Dry vs. Moist Skin

Comparison of Different Methods of Hyaluronic Acid Application on Skin Parameters

This study aims to compare the effects of two different methods of applying hyaluronic acid (HA) on skin parameters in healthy individuals. HA is a widely used ingredient in skincare products due to its hydrating properties. However, it is unclear whether applying HA to dry or pre-moistened skin provides better results in terms of skin hydration and texture.

In this non-invasive, interventional trial, 23 healthy volunteers aged 18 to 50 will be randomly assigned to two groups. One group will apply HA to dry skin, while the other will apply HA to moistened skin. Skin parameters will be measured before and after the intervention to assess hydration, transepidermal water loss, and overall skin condition.

The aim is to determine the optimal method of HA application that maximizes its effectiveness in improving skin quality. Participants may benefit from improved skin hydration and increased awareness of the best application method for their skin type. No significant risks are expected from participation.

Study Overview

• Status: Completed
• Conditions: Skin Hydration; Hyaluronic Acid
• Intervention / Treatment: Other: Topical Hyaluronic Acid

• Study Type: Interventional
• Enrollment (Actual): 23
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Croatia
  • Splitsko-dalmatinska županija
    • Split, Splitsko-dalmatinska županija, Croatia, 21000
      • University of Split School of Medicine

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Adult
• Accepts Healthy Volunteers: Yes

Description

Inclusion Criteria:

• Healthy individuals aged 18 to 50 years
• Willingness to apply the product daily for 3 weeks
• Ability to attend all scheduled skin assessments (baseline + weekly)
• Provided written informed consent

Exclusion Criteria:

• Known allergy or sensitivity to hyaluronic acid or any component of the product
• Active skin diseases or dermatological conditions in the facial area
• Use of systemic or topical corticosteroids or retinoids within 4 weeks prior to study
• Pregnancy or breastfeeding
• Participation in another clinical trial within the past 30 days

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: None (Open Label)

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Dry Skin Application; Participants in this group applied hyaluronic acid topically to clean, dry skin. The aim was to evaluate hydration and skin parameter changes following this method of application.	Other: Topical Hyaluronic Acid; Hyaluronic acid was applied topically to the facial skin of healthy participants once daily for a period of 3 weeks. Two application methods were used: on dry skin and on pre-moistened skin. Skin measurements were performed at baseline and after each week of intervention (Week 1, Week 2, and Week 3) to assess hydration and other skin parameters.
Experimental: Moist Skin Application; Participants in this group applied hyaluronic acid topically to pre-moistened skin. The goal was to assess whether applying the product to damp skin enhances its hydrating effect.	Other: Topical Hyaluronic Acid; Hyaluronic acid was applied topically to the facial skin of healthy participants once daily for a period of 3 weeks. Two application methods were used: on dry skin and on pre-moistened skin. Skin measurements were performed at baseline and after each week of intervention (Week 1, Week 2, and Week 3) to assess hydration and other skin parameters.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Change in skin hydration level	Skin hydration will be measured at four time points using a corneometer or comparable non-invasive skin analysis device. Measurements will be taken before the intervention (baseline) and at the end of each week to assess cumulative and comparative effects of hyaluronic acid application.	Baseline, Week 1, Week 2, Week 3

Collaborators and Investigators

• Sponsor: University of Split, School of Medicine

Study record dates

Study Major Dates

• Study Start (Actual): June 18, 2025
• Primary Completion (Actual): July 9, 2025
• Study Completion (Actual): July 9, 2025

Study Registration Dates

• First Submitted: July 11, 2025
• First Submitted That Met QC Criteria: July 11, 2025
• First Posted (Actual): July 22, 2025

Study Record Updates

• Last Update Posted (Actual): July 22, 2025
• Last Update Submitted That Met QC Criteria: July 11, 2025
• Last Verified: June 1, 2025

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Immunologic Factors; Physiological Effects of Drugs; Protective Agents; Adjuvants, Immunologic; Viscosupplements; Hyaluronic Acid
• Other Study ID Numbers: 2181-198-03-04-25-0064

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No