Effects of Platelet-Rich Fibrin After Mandibular Third Molar Surgery in Smokers and Nonsmokers

Effects of Platelet-Rich Fibrin on Postoperative Pain, Trismus and Swelling After Impacted Mandibular Third Molar Surgery in Smokers and Nonsmokers: A Prospective Clinical Study

Mandibular third molar surgery is commonly associated with postoperative pain, swelling, and trismus. Platelet-rich fibrin (PRF) has been proposed as a biological adjunct to improve postoperative healing; however, its effectiveness in smokers remains unclear. This prospective randomized split-mouth clinical study aimed to evaluate the effects of PRF on postoperative pain, swelling, trismus, and analgesic consumption after impacted mandibular third molar surgery in smokers and nonsmokers. Thirty patients with bilaterally impacted mandibular third molars were included. PRF was placed in one extraction socket, while the contralateral socket served as the control site. Postoperative clinical outcomes were assessed during the first postoperative week and compared between smoking and nonsmoking patients.

Study Overview

• Status: Completed
• Conditions: Postoperative Pain; Trismus; Facial Swelling; Impacted Mandibular Third Molar
• Intervention / Treatment: Biological: Platelet-Rich Fibrin

Detailed Description

This prospective randomized split-mouth clinical trial was conducted between 2017 and 2019 at the Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Ankara University. The study aimed to evaluate the effects of platelet-rich fibrin (PRF) on postoperative pain, swelling, trismus, and analgesic consumption after impacted mandibular third molar surgery in smoking and nonsmoking patients.

A total of 30 systemically healthy patients aged 18-35 years with bilaterally impacted mandibular third molars were included. Patients were divided into smoking and nonsmoking groups. In each patient, PRF was randomly applied to one extraction socket, while the contralateral socket served as the control site without PRF application. All surgical procedures were performed by the same oral and maxillofacial surgeon using a standardized surgical protocol. Outcome assessments were performed by a different clinician blinded to the intervention side.

Postoperative pain was evaluated using a visual analog scale (VAS). Facial swelling was assessed using linear facial measurements between anatomical landmarks, and trismus was evaluated by measuring maximum interincisal distance. Clinical evaluations were performed preoperatively and on postoperative days 1, 2, 3, and 7.

The primary outcome of the study was postoperative pain intensity. Secondary outcomes included postoperative swelling, trismus, and analgesic consumption. The study also aimed to compare the clinical effectiveness of PRF between smokers and nonsmokers.

• Study Type: Interventional
• Enrollment (Actual): 30
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Turkey (Türkiye)
  • Ankara, Turkey (Türkiye), 06560
    • Ankara University Faculty of Dentistry

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Adult
• Accepts Healthy Volunteers: No

Description

Inclusion Criteria:

• Systemically healthy patients aged between 18 and 35 years
• Bilaterally impacted mandibular third molars
• Mandibular third molars classified as Pell and Gregory Class I, Position C
• Mandibular third molars classified as Parant Class II difficulty
• Smoking and nonsmoking patients
• Patients willing to participate and provide written informed consent

Exclusion Criteria:

• Presence of acute infection or pericoronitis
• Pregnancy or lactation
• Alcohol or substance abuse
• Use of oral contraceptives
• Menstrual period during surgery
• Antibiotic use within the previous month
• Smoking more than 10 cigarettes per day
• Agitation or inability to cooperate
• Incomplete follow-up data
• Systemic disease contraindicating oral surgery

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: Single

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: PRF-treated socket; Platelet-rich fibrin (PRF) was placed into the extraction socket following impacted mandibular third molar surgery.	Biological: Platelet-Rich Fibrin; Autologous platelet-rich fibrin prepared from venous blood by centrifugation and placed into the extraction socket after mandibular third molar extraction.
No Intervention: Control socket; The contralateral extraction socket was primarily closed without PRF application after mandibular third molar surgery.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Postoperative Pain Intensity	Postoperative pain intensity assessed using a 100-mm visual analog scale (VAS) after impacted mandibular third molar surgery.	Postoperative days 1, 2, 3, and 7

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Postoperative Facial Swelling	Postoperative facial swelling assessed using linear facial measurements between anatomical landmarks.	Preoperative period and postoperative days 1, 2, 3, and 7
Postoperative Trismus	Trismus assessed by measuring maximum interincisal mouth opening distance.	Preoperative period and postoperative days 1, 2, 3, and 7
Postoperative Analgesic Consumption	Number of analgesic tablets used during the postoperative follow-up period.	Postoperative days 1, 2, 3, and 7

Collaborators and Investigators

• Sponsor: Ankara University
• Investigators: Principal Investigator: Mehmet Emre Yurttutan, PhD, DDS, Ankara University, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery

Study record dates

Study Major Dates

• Study Start (Actual): May 15, 2017
• Primary Completion (Actual): December 21, 2018
• Study Completion (Actual): December 21, 2018

Study Registration Dates

• First Submitted: May 17, 2026
• First Submitted That Met QC Criteria: May 17, 2026
• First Posted (Actual): May 22, 2026

Study Record Updates

• Last Update Posted (Actual): May 22, 2026
• Last Update Submitted That Met QC Criteria: May 17, 2026
• Last Verified: May 1, 2026

More Information

Terms related to this study

• Keywords: Postoperative Pain; Smoking; Swelling; Platelet-Rich Fibrin; Trismus; Mandibular Third Molar
• Additional Relevant MeSH Terms: Pain; Neurologic Manifestations; Nervous System Diseases; Neuromuscular Manifestations; Postoperative Complications; Pathologic Processes; Spasm; Pathological Conditions, Signs and Symptoms; Behavior; Signs and Symptoms; Pain, Postoperative; Trismus; Smoking
• Other Study ID Numbers: 36290600/51

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No