Evaluation of Single and Repeat Doses of GSK729327 in Healthy Volunteers

Inclusion Criteria:

• Healthy male subjects between the ages of 18 and 55 years inclusive.
• Part B also includes healthy female subjects of non-child bearing potential.
• A normal ECG, physical examination and laboratory screen.
• Body weight >50 kg and BMI within the range 18.5-29.9 kg/m2 inclusive.
• Signed and dated written informed consent prior to admission to the study.

Exclusion Criteria:

• Abuse of alcohol.
• A positive pre-study urine drug or alcohol screen.
• A positive pre-study HIV, Hepatitis B surface antigen or positive Hepatitis C antibody result at screening.
• History of DSM-IV alcohol and/or drug abuse or dependence.
• Consumption of grapefruit juice or grapefruit within 7 days prior to the first dose of study medication until final evaluation.
• Has received an investigational drug or has participated in any other research trial within 30 days or 5 half-lives, or twice the duration of the biological effect of any drug (which ever is longer) prior to the first dose of study medication.
• Exposure to more than four new chemical entities within 12 months prior to the first dosing day.
• Use of prescription or non-prescription drugs within 4 weeks prior to the first dose of study medication until final evaluation. Where participation in study would result in donation of blood in excess of 500 ml within a 56 day period.
• History or presence of allergy to the study drug or drugs of this class, or a history of drug or other allergy that, in the opinion of the physician responsible, contraindicates their participation.
• History or presence of clinically significant gastro-intestinal, hepatic or renal disease or other condition known to interfere with the absorption, distribution, metabolism or excretion of drugs.
• History of cholecystectomy or biliary tract disease.
• History of regular use of tobacco- or nicotine-containing products within 6 months of the start of a study.
• An unwillingness of the male subject to use a condom/spermicide in addition to having their female partner use another form of contraception such as an IUD, diaphragm with spermicide, oral contraceptives, injectable progesterone, subdermal implants or a tubal ligation if the woman could become pregnant from the time of the first dose of study medication until 90 days following administration of the last dose of study medication.QTc interval > 450 ms. Or an ECG that is not suitable for QT measurements.
• History of long QT syndrome (personal or family) or other cardiac conduction disorder, or other clinically significant cardiac disease.
• The subject has a history of myocardial infarction .The subject has a resting pulse rate <55 or >100 bpm OR a systolic blood pressure >140 or <100 OR a diastolic blood pressure >90 or <60.Current or past history of symptomatic orthostatic hypotension. The subject has any laboratory abnormality that in the investigator's judgment is considered to be clinically significant (even if not outside of specified ranges).History of known or suspected seizures, including infantile febrile, unexplained significant and recent loss of consciousness or history of significant head trauma with loss of consciousness or a family history (first degree relative) of epilepsy or seizures (fits).History or presence of psychiatric disease.
• History of suicidal attempts or behavior. Subjects who cannot complete the neuropsychological test battery despite having undergone the training sessions.
• Subject has a history of sleep problems in the last 3 months.
• The subject has clinically significant abnormalities in haematology, blood chemistry, ECG, or physical examination not resolved by the screening visit. Abnormal response to photic stimulation EEG. Abnormal prolactin or TSH or free T4 or free T3 at screening/baseline.