Effects of periodontal therapy on GCF cytokines in generalized aggressive periodontitis subjects
Ana Paula de Lima Oliveira, Marcelo de Faveri, Lauren Christine Gursky, Maria Josefa Mestnik, Magda Feres, Anne D Haffajee, Sigmund S Socransky, Ricardo Palmier Teles, Ana Paula de Lima Oliveira, Marcelo de Faveri, Lauren Christine Gursky, Maria Josefa Mestnik, Magda Feres, Anne D Haffajee, Sigmund S Socransky, Ricardo Palmier Teles
Abstract
Aim: To examine changes in levels of gingival crevicular fluid (GCF) cytokines, after periodontal therapy of generalized aggressive periodontitis (GAgP).
Materials and methods: Twenty-five periodontally healthy and 24 GAgP subjects had periodontal clinical parameters measured and gingival crevicular fluid (GCF) samples collected from up to 14 sites/subject. GCF samples were analysed using multiplex bead immunoassay for: GM-CSF, IFN-γ, IL-10, IL-1β, IL-2, IL-6 and TNF-α. Aggressive periodontitis subjects were randomly assigned to either scaling and root planing (SRP) alone or SRP plus systemic amoxicillin (500 mg) and metronidazole (400 mg) 3 times a day for 14 days. Clinical parameters and GCF cytokines were re-measured 6 months after treatment. Differences over time were analysed using the Wilcoxon test and between groups using the Mann-Whitney test.
Results: Significant reductions in GCF GM-CSF, IL-1β and the ratio IL-1β/IL-10 and increases in GCF IL-6 were detected after therapy. The mean change in GCF cytokines did not differ significantly between groups.
Conclusions: Periodontal therapy improved GCF cytokine profiles by lowering IL-1β and increasing IL-10 levels. The reduction in GCF GM-CSF after therapy implicates this cytokine in the pathogenesis of GAgP. There was no difference between therapies in changes of GCF cytokines.
Conflict of interest statement
Conflict of Interest
The authors declare that they have no conflict of interests.
© 2011 John Wiley & Sons A/S.
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Source: PubMed