Interleukin-10 polymorphisms affect the key periodontal pathogens in Chinese periodontitis patients

Ying Geng, Lu Li, Xiaoqian Wang, Fanzhen He, Yi Zhou, Mifang Yang, Yan Xu, Ying Geng, Lu Li, Xiaoqian Wang, Fanzhen He, Yi Zhou, Mifang Yang, Yan Xu

Abstract

Interleukin-10 (IL-10) polymorphisms have been shown to affect IL-10 production. This study investigated the influences of IL-10 polymorphisms on the susceptibility to chronic periodontitis (CP) and aggressive periodontitis (AP), and their possible role in the quantity of subgingival bacteria Aggregatibacter Actinomycetemcomitans and Porphyromonas gingivalis. 92 CP patients, 83 AP patients and 91 periodontal healthy controls were recruited. Serum IL-10 concentration was analyzed by enzyme-linked immunosorbent assay (ELISA). Gene polymorphisms were determined by multiplex SNaPshot technique. Bacteria were quantified by real-time polymerase chain reaction with TaqMan MGB probes. Taking into account age, gender and periodontal status, IL-10-592 AA, -819 TT and ATA/ATA genotype occurred more frequently in patients with CP than in healthy controls. In CP cases, higher quantity of subgingival A. actinomycetemcomitans and lower serum IL-10 levels could be detected in homozygous ATA/ATA carriers. These findings indicate that variants in IL-10 promoter gene were not only associated with predisposition to chronic periodontitis but also affected the subgingival number of A. Actinomycetemcomitans in a Chinese Han population.

Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Bacterial counts (through logarithmic transformation) of A. actinomycetemcomitans and P. gingivalis. No significance (NS) for both pathogens by Mann-Whitney U test between CP and AP groups. Patients from CP and AP groups showed significant higher number of both pathogens compared with PH controls (P < 0.001 by Mann-Whitney U test). **Significant (p < 0.001 by Mann-Whitney U test) PH, periodontal healthy; CP, chronic periodontitis; AP, aggressive periodontitis.
Figure 2
Figure 2
Association between SNPs in IL-10 promoter gene (IL-10-592, -819 and -1082 genotype) and the subgingival number of A. actinomycetemcomitans in CP group. *Significant (p < 0.05 by Kruskal-Wallis ANOVA test followed by Dunn-Bonferroni test) CP, chronic periodontitis.
Figure 3
Figure 3
Association between the IL-10 haplotype and the subgingival number of A. actinomycetemcomitans in CP group. *Significant (p < 0.05 by Mann-Whitney U test) CP, chronic periodontitis.

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