Nicotine-induced cessation of embryonic development is reversed by γ-tocotrienol in mice

Yuhaniza Shafinie Kamsani, Mohd Hamim Rajikin, Nor-Ashikin Mohamed Nor Khan, Nuraliza Abdul Satar, Amar Chatterjee, Yuhaniza Shafinie Kamsani, Mohd Hamim Rajikin, Nor-Ashikin Mohamed Nor Khan, Nuraliza Abdul Satar, Amar Chatterjee

Abstract

Background: This study aimed to evaluate the adverse effects of various doses of nicotine and protective effects of different concentrations of gamma-tocotrienol (gamma-TCT) on in vitro embryonic development and lipid peroxidation in mice.

Material and methods: A) Effects of various doses of nicotine on in vitro embryonic development: Female mice were treated with 1.0, 3.0, or 5.0 mg/kg/day nicotine for 7 consecutive days. Animals were superovulated, cohabited overnight, and sacrificed. Embryos were cultured in vitro. Plasma was assayed. B) Effects of concomitant treatment of nicotine concurrently with various doses of gamma-TCT on in vitro embryonic development: Female mice were treated with nicotine (5.0 mg/kg/day), gavaged gamma-TCT of 30, 60, or 90 mg/kg/day or nicotine concurrently with gamma-TCT of 3 different doses for 7 consecutive days. Animals were superovulated, cohabited overnight, and sacrificed. Embryos were cultured and plasma was assayed.

Results: A) Effects of various doses of nicotine on in vitro embryonic development: Number of hatched blastocysts decreased in 1.0 and 3.0 mg/kg/day nicotine groups. Nicotine at 5.0 mg/kg/day stopped embryo development at morula. MDA concentrations increased following all nicotine doses. B) Effects of concomitant treatment of nicotine concurrently with various doses of gamma-TCT on in vitro embryonic development: Embryo development was completed in all groups. MDA concentration increased only in the group treated with nicotine concurrently with 30 mg/kg/day gamma-TCT.

Conclusions: Nicotine impairs in vitro embryo development and increases MDA in plasma. The deleterious impact of nicotine on embryo development is reversed by supplementing gamma-TCT concurrently with nicotine.

Figures

Figure 1
Figure 1
Number of preimplantation embryos developed following different doses of nicotine in mice. a – p

Figure 2

Preimplantation embryo development in G1,…

Figure 2

Preimplantation embryo development in G1, G2, G3 and G6. a, b – p…

Figure 2
Preimplantation embryo development in G1, G2, G3 and G6. a, b – p

Figure 3

Preimplantation embryo development in G1,…

Figure 3

Preimplantation embryo development in G1, G2, G4 and G7. a – p

Figure 3
Preimplantation embryo development in G1, G2, G4 and G7. a – p

Figure 4

Preimplantation embryo development in G1,…

Figure 4

Preimplantation embryo development in G1, G2, G5 and G8. a – p

Figure 4
Preimplantation embryo development in G1, G2, G5 and G8. a – p

Figure 5

Plasma MDA levels following different…

Figure 5

Plasma MDA levels following different doses of nicotine. a – p

Figure 5
Plasma MDA levels following different doses of nicotine. a – p

Figure 6

Plasma MDA levels following concomitant…

Figure 6

Plasma MDA levels following concomitant administration of nicotine concurrently with various doses of…

Figure 6
Plasma MDA levels following concomitant administration of nicotine concurrently with various doses of γ-TCT. a – p
Similar articles
References
    1. Wang X, Falcone T, Attaran M, et al. Vitamin C and Vitamin E supplementation reduce oxidative stress-induced embryo toxicity and improve the blastocysts development rate. Fert Steril. 2002;78:1271–77. - PubMed
    1. Rajikin MH, Latif ES, Mar MR, et al. Deleterious effects of nicotine on the ultrastructure of oocytes: role of gamma tocotrienol. Med Sci Monit. 2009;15(12):BR378–83. - PubMed
    1. Tarin JJ. Potential effects of age-associated oxidative stress on mammalian oocytes/embryos. Mol Hum R Prod. 1996;2:717–24. - PubMed
    1. Kamsani YS, Rajikin MH, Chatterjee A, et al. Impairment of in vitro embryonic development with a corresponding elevation of oxidative stress following nicotine treatment in mice: effect of variation in treatment duration. Biomed Res. 2010;21(4):359–64.
    1. Mokhtar N, Rajikin MH, Zakaria Z. Role of tocotrienol-rich palm vitamin E on pregnancy and preimplantation embryos in nicotine treated rats. Biomed Res. 2008;19:181–84.
Show all 24 references
Publication types
MeSH terms
[x]
Cite
Copy Download .nbib
Format: AMA APA MLA NLM

NCBI Literature Resources

MeSH PMC Bookshelf Disclaimer

The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Unauthorized use of these marks is strictly prohibited.

Follow NCBI
Figure 2
Figure 2
Preimplantation embryo development in G1, G2, G3 and G6. a, b – p

Figure 3

Preimplantation embryo development in G1,…

Figure 3

Preimplantation embryo development in G1, G2, G4 and G7. a – p

Figure 3
Preimplantation embryo development in G1, G2, G4 and G7. a – p

Figure 4

Preimplantation embryo development in G1,…

Figure 4

Preimplantation embryo development in G1, G2, G5 and G8. a – p

Figure 4
Preimplantation embryo development in G1, G2, G5 and G8. a – p

Figure 5

Plasma MDA levels following different…

Figure 5

Plasma MDA levels following different doses of nicotine. a – p

Figure 5
Plasma MDA levels following different doses of nicotine. a – p

Figure 6

Plasma MDA levels following concomitant…

Figure 6

Plasma MDA levels following concomitant administration of nicotine concurrently with various doses of…

Figure 6
Plasma MDA levels following concomitant administration of nicotine concurrently with various doses of γ-TCT. a – p
Similar articles
References
    1. Wang X, Falcone T, Attaran M, et al. Vitamin C and Vitamin E supplementation reduce oxidative stress-induced embryo toxicity and improve the blastocysts development rate. Fert Steril. 2002;78:1271–77. - PubMed
    1. Rajikin MH, Latif ES, Mar MR, et al. Deleterious effects of nicotine on the ultrastructure of oocytes: role of gamma tocotrienol. Med Sci Monit. 2009;15(12):BR378–83. - PubMed
    1. Tarin JJ. Potential effects of age-associated oxidative stress on mammalian oocytes/embryos. Mol Hum R Prod. 1996;2:717–24. - PubMed
    1. Kamsani YS, Rajikin MH, Chatterjee A, et al. Impairment of in vitro embryonic development with a corresponding elevation of oxidative stress following nicotine treatment in mice: effect of variation in treatment duration. Biomed Res. 2010;21(4):359–64.
    1. Mokhtar N, Rajikin MH, Zakaria Z. Role of tocotrienol-rich palm vitamin E on pregnancy and preimplantation embryos in nicotine treated rats. Biomed Res. 2008;19:181–84.
Show all 24 references
Publication types
MeSH terms
[x]
Cite
Copy Download .nbib
Format: AMA APA MLA NLM

NCBI Literature Resources

MeSH PMC Bookshelf Disclaimer

The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Unauthorized use of these marks is strictly prohibited.

Follow NCBI
Figure 3
Figure 3
Preimplantation embryo development in G1, G2, G4 and G7. a – p

Figure 4

Preimplantation embryo development in G1,…

Figure 4

Preimplantation embryo development in G1, G2, G5 and G8. a – p

Figure 4
Preimplantation embryo development in G1, G2, G5 and G8. a – p

Figure 5

Plasma MDA levels following different…

Figure 5

Plasma MDA levels following different doses of nicotine. a – p

Figure 5
Plasma MDA levels following different doses of nicotine. a – p

Figure 6

Plasma MDA levels following concomitant…

Figure 6

Plasma MDA levels following concomitant administration of nicotine concurrently with various doses of…

Figure 6
Plasma MDA levels following concomitant administration of nicotine concurrently with various doses of γ-TCT. a – p
Similar articles
References
    1. Wang X, Falcone T, Attaran M, et al. Vitamin C and Vitamin E supplementation reduce oxidative stress-induced embryo toxicity and improve the blastocysts development rate. Fert Steril. 2002;78:1271–77. - PubMed
    1. Rajikin MH, Latif ES, Mar MR, et al. Deleterious effects of nicotine on the ultrastructure of oocytes: role of gamma tocotrienol. Med Sci Monit. 2009;15(12):BR378–83. - PubMed
    1. Tarin JJ. Potential effects of age-associated oxidative stress on mammalian oocytes/embryos. Mol Hum R Prod. 1996;2:717–24. - PubMed
    1. Kamsani YS, Rajikin MH, Chatterjee A, et al. Impairment of in vitro embryonic development with a corresponding elevation of oxidative stress following nicotine treatment in mice: effect of variation in treatment duration. Biomed Res. 2010;21(4):359–64.
    1. Mokhtar N, Rajikin MH, Zakaria Z. Role of tocotrienol-rich palm vitamin E on pregnancy and preimplantation embryos in nicotine treated rats. Biomed Res. 2008;19:181–84.
Show all 24 references
Publication types
MeSH terms
[x]
Cite
Copy Download .nbib
Format: AMA APA MLA NLM

NCBI Literature Resources

MeSH PMC Bookshelf Disclaimer

The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Unauthorized use of these marks is strictly prohibited.

Follow NCBI
Figure 4
Figure 4
Preimplantation embryo development in G1, G2, G5 and G8. a – p

Figure 5

Plasma MDA levels following different…

Figure 5

Plasma MDA levels following different doses of nicotine. a – p

Figure 5
Plasma MDA levels following different doses of nicotine. a – p

Figure 6

Plasma MDA levels following concomitant…

Figure 6

Plasma MDA levels following concomitant administration of nicotine concurrently with various doses of…

Figure 6
Plasma MDA levels following concomitant administration of nicotine concurrently with various doses of γ-TCT. a – p
Similar articles
References
    1. Wang X, Falcone T, Attaran M, et al. Vitamin C and Vitamin E supplementation reduce oxidative stress-induced embryo toxicity and improve the blastocysts development rate. Fert Steril. 2002;78:1271–77. - PubMed
    1. Rajikin MH, Latif ES, Mar MR, et al. Deleterious effects of nicotine on the ultrastructure of oocytes: role of gamma tocotrienol. Med Sci Monit. 2009;15(12):BR378–83. - PubMed
    1. Tarin JJ. Potential effects of age-associated oxidative stress on mammalian oocytes/embryos. Mol Hum R Prod. 1996;2:717–24. - PubMed
    1. Kamsani YS, Rajikin MH, Chatterjee A, et al. Impairment of in vitro embryonic development with a corresponding elevation of oxidative stress following nicotine treatment in mice: effect of variation in treatment duration. Biomed Res. 2010;21(4):359–64.
    1. Mokhtar N, Rajikin MH, Zakaria Z. Role of tocotrienol-rich palm vitamin E on pregnancy and preimplantation embryos in nicotine treated rats. Biomed Res. 2008;19:181–84.
Show all 24 references
Publication types
MeSH terms
[x]
Cite
Copy Download .nbib
Format: AMA APA MLA NLM

NCBI Literature Resources

MeSH PMC Bookshelf Disclaimer

The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Unauthorized use of these marks is strictly prohibited.

Follow NCBI
Figure 5
Figure 5
Plasma MDA levels following different doses of nicotine. a – p

Figure 6

Plasma MDA levels following concomitant…

Figure 6

Plasma MDA levels following concomitant administration of nicotine concurrently with various doses of…

Figure 6
Plasma MDA levels following concomitant administration of nicotine concurrently with various doses of γ-TCT. a – p
Similar articles
References
    1. Wang X, Falcone T, Attaran M, et al. Vitamin C and Vitamin E supplementation reduce oxidative stress-induced embryo toxicity and improve the blastocysts development rate. Fert Steril. 2002;78:1271–77. - PubMed
    1. Rajikin MH, Latif ES, Mar MR, et al. Deleterious effects of nicotine on the ultrastructure of oocytes: role of gamma tocotrienol. Med Sci Monit. 2009;15(12):BR378–83. - PubMed
    1. Tarin JJ. Potential effects of age-associated oxidative stress on mammalian oocytes/embryos. Mol Hum R Prod. 1996;2:717–24. - PubMed
    1. Kamsani YS, Rajikin MH, Chatterjee A, et al. Impairment of in vitro embryonic development with a corresponding elevation of oxidative stress following nicotine treatment in mice: effect of variation in treatment duration. Biomed Res. 2010;21(4):359–64.
    1. Mokhtar N, Rajikin MH, Zakaria Z. Role of tocotrienol-rich palm vitamin E on pregnancy and preimplantation embryos in nicotine treated rats. Biomed Res. 2008;19:181–84.
Show all 24 references
Publication types
MeSH terms
[x]
Cite
Copy Download .nbib
Format: AMA APA MLA NLM
Figure 6
Figure 6
Plasma MDA levels following concomitant administration of nicotine concurrently with various doses of γ-TCT. a – p

References

    1. Wang X, Falcone T, Attaran M, et al. Vitamin C and Vitamin E supplementation reduce oxidative stress-induced embryo toxicity and improve the blastocysts development rate. Fert Steril. 2002;78:1271–77.
    1. Rajikin MH, Latif ES, Mar MR, et al. Deleterious effects of nicotine on the ultrastructure of oocytes: role of gamma tocotrienol. Med Sci Monit. 2009;15(12):BR378–83.
    1. Tarin JJ. Potential effects of age-associated oxidative stress on mammalian oocytes/embryos. Mol Hum R Prod. 1996;2:717–24.
    1. Kamsani YS, Rajikin MH, Chatterjee A, et al. Impairment of in vitro embryonic development with a corresponding elevation of oxidative stress following nicotine treatment in mice: effect of variation in treatment duration. Biomed Res. 2010;21(4):359–64.
    1. Mokhtar N, Rajikin MH, Zakaria Z. Role of tocotrienol-rich palm vitamin E on pregnancy and preimplantation embryos in nicotine treated rats. Biomed Res. 2008;19:181–84.
    1. Rozzana MS, Zaiton Z, Rajikin MH, et al. Supplementation with alpha lipoic acid improves the in vitro development of embryos in nicotine-treated mice. Biomed Res. 2005;16:28–32.
    1. Talbot P, Riveles K. Smoking and reproduction: the oviduct as a target of cigarette smoke. Reprod Biol Endocrinol. 2005;3:1–17.
    1. Agarwal A, Gupta S, Sharma RK. Role of oxidative stress in female reproduction. Reprod Biol Endocr. 2005;3:1–21.
    1. Ashakumary L, Vijayammal PL. Effect of nicotine on antioxidant defense mechanisms in rats fed with a high-fat diet. Pharmacol. 1996;52:153–58.
    1. Raederstorff D, Elste V, Aebischer C, et al. Effect of either gamma-tocotrienol or a tocotrienol mixture on the plasma lipid profile in hamsters. Ann Nutr Metab. 2002;46:17–23.
    1. Aggarwal BB, Sundaram C, Prasad S, et al. Tocotrienols, the vitamin E of the 21st century: Its potential against cancer and other chronic diseases. Biochem Pharma. 2010;80:1613–31.
    1. Qureshi AA, Bradlow BA, Brace L, et al. Response of hypercholesterolemic subjects to administration of tocotrienols. Lipids. 1995;30:1171–77.
    1. Sen CK, Khanna S, Roy S. Tocotrienols in health and disease: the other half of the natural vitamin E family. Mol Aspects Med. 2007;28:692–728.
    1. Ertzeid G, Storeng R. Adverse effects of gonadotrophin treatment on pre- and postimplantation development in mice. J Reprod Fert. 1992;96:649–55.
    1. Lefevre G, Leymarie B, Beyerle M, et al. Evaluation of lipid peroxidation by measuring thiobarbituric acid reactive substances. Ann Biol Clin. 1998;56:305–19.
    1. Freour T, Masson D, Mirallie S, et al. Active smoking compromises IVF outcome and affects ovarian reserve. Reprod Biomed. 2008;16:96–102.
    1. Berthiller J, Sasco AJ. Smoking (active or passive) in relation to fertility. J Gynecol Obstet Biol Reprod. 2005;34(1):47–54.
    1. Joesbury KA, Edirisinghe WR, Phillips MR, et al. Evidence that male smoking affects the likelihood of a pregnancy following IVF treatment: application of the modified cumulative embryo score. Hum Reprod. 1998;13:1506–13.
    1. Lockman PR, Van der Schyf CJ, Abbruscato TJ, et al. Chronic nicotine exposure alters blood-brain barrier permeability and diminishes brain uptake of methyllycaconitine. J Neurochem. 2005;94:37–44.
    1. Mitchell JA, Hammer RE. Effects of nicotine on oviductal blood flow and embryo development in the rat. J Reprod Fertil. 1985;74:71–76.
    1. Card JP, Mitchell JA. The effects of nicotine on implantation in the rat. Biol Reprod. 1979;20:532–39.
    1. Sieber SM, Fabro S. Identification of drugs in the preimplantation blastocyst and in the plasma, uterine secretion and and urine of the pregnant rabbit. J Pharmacol Exp Ther. 1971;176(1):65–75.
    1. Raederstorff D, Elste V, Aebischer C, et al. Effect of either gamma-tocotrienol or a tocotrienol mixture on the plasma lipid profile in hamsters. Ann Nutr Metab. 2002;46:17–23.
    1. Hermizi H, Faizah O, Nirwana SI, et al. Beneficial effects of tocotrienol and tocopherol on bone histomorphometric parameters in Sprague-Dawley male rats after nicotine cessation. Calcif Tissue Intl. 2009;84:65–74.

Source: PubMed

Sponsorer og samarbejdspartnere

Medicinske tilstande

Narkotikainterventioner

3
Abonner