Nanotechnology in ligature-induced periodontitis: protective effect of a doxycycline gel with nanoparticules
Marco Antonio Botelho, Jose Galberto Martins, Ronaldo Sousa Ruela, Dinalva Brito Queiroz, Wagner Sousa Ruela, Marco Antonio Botelho, Jose Galberto Martins, Ronaldo Sousa Ruela, Dinalva Brito Queiroz, Wagner Sousa Ruela
Abstract
Objectives: The aim of this study was to test the efficacy of a locally applied 8.5% nanostructured doxycycline (DOX) gel in preventing alveolar bone loss in experimental periodontal disease (EPD) in rats by using the tapping mode atomic force microscopy (AFM).
Material and methods: EPD was induced in 24 Wistar rats. Animals were treated with the doxycycline gel topically, immediately after EPD induction, and 3 times a day during 11 days. Four groups (n=6) were formed as follows: Naïve group (animals not subjected to EPD nor treated); non-treated (NT) group (animals subjected to EPD, but not treated); vehicle gel (VG) group (animals subjected to EPD and treated with topical gel vehicle); and DOX group (test group): animals subjected to EPD and treated with the 8.5% DOX gel. In order to investigate topographical changes in histological sections, a novel simple method was used for sample preparation, by etching sections from paraffin-embedded specimens with xylol.
Results: Comparing the AFM images, several grooves were observed on the surface of the alveolar bone and other periodontal structures in the NT and VG groups, with significantly greater depths when compared to the DOX group (p<0.05).
Conclusions: Periodontal structures were brought into high relief confirming to be a simple and cost-effective method for AFM imaging with ultrastructural resolution. The doxycycline gel was able to afford periodontal surface preservation, with flatter grooves.
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References
- Armada-Dias L, Breda J, Provenzano JC, Breitenbach M, Rôças IN, Gahyva SM, et al. Development of periradicular lesions in normal and diabetic rats. J Appl Oral Sci. 2006;14(5):371–375.
- Bezerra MM, Brito GA, Ribeiro RA, Rocha FA. Low-dose doxycycline prevents inflammatory bone resorption in rats. Braz J Med Biol Res. 2002;35(5):613–616.
- Bosco JMD, Oliveira SR, Bosco AF, Schweitzer CM, Jardim EG., Jr Influence of local tetracycline on the microbiota of alveolar osteitis in rats. Braz Dent J. 2008;19(2):119–123.
- Botelho MA, Bezerra JG, Filho, Correa LL, Fonseca SG, Montenegro D, Gapski R, et al. Effect of a novel essential oil mouthrinse without alcohol on gingivitis: a double-blinded randomized controlled trial. J Appl Oral Sci. 2007;15(3):175–180.
- Botelho MA, Martins JG, Ruela RS, Santos JA, Soares JB, França MC, et al. Protective effect of locally applied carvacrol gel on ligature-induced periodontitis in rats: a tapping mode AFM study. Phytother Res. 2009;25(10):1439–1448.
- Botelho MA, Nogueira NA, Bastos GM, Fonseca SG, Lemos TL, Matos FJ, et al. Antimicrobial activity of the essential oil from Lippia sidoides, carvacrol and thymol against oral pathogens. Braz J Med Biol Res. 2007;40(3):349–356.
- Botelho MA, Rao VS, Carvalho CB, Bezerra-Filho JG, Fonseca SG, Vale BM, et al. Lippia sidoides and Myracrodruon urundeuva gel prevents alveolar bone resorption in experimental periodontitis in rats. J Ethnopharmacol. 2007;113(3):471–478.
- Botelho MA, Rao VS, Montenegro D, Bandeira MA, Fonseca SG, Nogueira NA, et al. Effects of a herbal gel containing carvacrol and chalcones on alveolar bone resorption in rats on experimental periodontitis. Phytother Res. 2008;22(4):442–449.
- Botelho MA, Santos RA, Martins JG, Carvalho CO, Paz MC, Azenha C, et al. Comparative effect of an essential oil mouthrinse on plaque, gingivitis and salivary Streptococcus mutans levels: a double blind randomized study. Phytother Res. 2009;23(9):1214–1219.
- Costa JG, Rodrigues GF, Botelho MA. Antibacterial properties of pequi pulp oil (Caryocar coriaceum-Wittm.) Int J Food Prop. 2009 (In press)
- Digital Instruments Veeco Metrology Group. Command Reference Manual. Software version 5.12 r3; 2001.
- Golub LM, Lee HM, Greenwald RA, Ryan Me, Sorsa T, Salo T, et al. A matrix metalloproteinase inhibitor reduces bone-type collagen degradation fragments and specific collagenases in gingival crevicular fluid during adult periodontitis. Inflamm Res. 1997;46(2):310–319.
- Golub LM, McNamara TF, Ryan Me, Kohut B, Blieden T, Payonk G, et al. Adjunctive treatment with subantimicrobial doses of doxycycline: effects on gingival fluid collagenase activity and attachment loss in adult periodontitis. J Clin Periodontol. 2001;28(4):146–156.
- Golub LM, Sorsa T, Lee HM, Ciancio S, Sorbi D, Ramamurthy NS, et al. Doxycycline inhibits neutrophil (PMN)-type matrix metalloproteinases in human adult periodontitis gingiva. J Clin Periodontol. 1995;(22):100–109.
- Llavaneras A, Golub LM, Rifkin BR, Heikkila P, Sorsa T, Teronen O, et al. CMT-8/clodronate combination therapy synergistically inhibits alveolar bone loss in LPS-induced periodontitis. Ann N Y Acad Sci. 1999;878:671–674.
- Machion L, Andia DC, Lecio G, Nociti FH, Casati MZ, Sallum AW, et al. Locally delivered doxycycline as an adjunctive therapy to scaling and root planing in the treatment of smokers: a 2-year follow-up. J Periodontol. 2006;77(4):606–613.
- Morris VJ, Kirby AR, Gunning AP. Atomic force microscopy for biologists. London: Imperial College Press; 2000.
- Novak MJ, Johns LP, Miller RC, Bradshaw MH. Adjunctive benefits of subantimicrobial dose doxycycline in the management of severe, generalized, chronic periodontitis. J Periodontol. 2002;73(7):762–769.
- Ramamurthy NS, Rifkin BR, Greenwald RA, Xu JW, Liu Y, Turner LG, et al. Inhibition of matrix metalloproteinase-mediated periodontal bone loss in rats: a comparison of 6 chemically modified tetracyclines. J Periodontol. 2002;73(7):726–734.
- Ryder MI, Pons B, Adams D, Beiswanger B, Blanco V, Bogle G, et al. Effects of smoking on local delivery of controlled-release doxycycline as compared to scaling and root planing. J Clin Periodontol. 1999;26(2):683–691.
- Santos CF, Sakai VT, Machado MAAM, Schippers DN, Greene AS. Reverse transcription and polymerase chain reaction: principles and applications in dentistry. J Appl Oral Sci. 2004;12(1):1–11.
- Sayles RS. The profile as a random process. In: Thomas TR, editor. Rough surfaces. London: Longman; 1982.
- Stoller NH, Johnson LR, Trapnell S, Harrold CQ, Garrett S. The pharmacokinetic profile of a biodegradable controlled-release delivery system containing doxycycline compared to systemically delivered doxycycline in gingival crevicular fluid, saliva, and serum. J Periodontol. 1998;69(10):1085–1091.
- Stoltze K. Concentration of metronidazole in periodontal pockets after application of a metronidazole 25% dental gel. Pt 2J Clin Periodontol. 1992;19(9):698–701.
Source: PubMed