Metabolic Biosynthesis Pathways Identified from Fecal Microbiome Associated with Prostate Cancer
Michael A Liss, James Robert White, Martin Goros, Jonathan Gelfond, Robin Leach, Teresa Johnson-Pais, Zhao Lai, Elizabeth Rourke, Joseph Basler, Donna Ankerst, Dimpy P Shah, Michael A Liss, James Robert White, Martin Goros, Jonathan Gelfond, Robin Leach, Teresa Johnson-Pais, Zhao Lai, Elizabeth Rourke, Joseph Basler, Donna Ankerst, Dimpy P Shah
Abstract
Background: The fecal microbiome is associated with prostate cancer risk factors (obesity, inflammation) and can metabolize and produce various products that may influence cancer but have yet to be defined in prostate cancer.
Objective: To investigate gut bacterial diversity, identify specific metabolic pathways associated with disease, and develop a microbiome risk profile for prostate cancer.
Design, setting, and participants: After prospective collection of 133 rectal swab samples 2 wk before the transrectal prostate biopsy, we perform 16S rRNA amplicon sequencing on 105 samples (64 with cancer, 41 without cancer). Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) was applied to infer functional categories associated with taxonomic composition. The p values were adjusted using the false discovery rate. The α- and β-diversity analyses were performed using QIIME. The Mann-Whitney U test was employed to evaluate the statistical significance of β-diversity distances within and between groups of interest, and least absolute shrinkage and selection operator (LASSO) regression analysis was used to determine pathway significance.
Outcome measurements and statistical analysis: The detection of prostate cancer on transrectal prostate needle biopsy and 16s microbiome profile.
Results and limitations: We identified significant associations between total community composition and cancer/non-cancer status (Bray-Curtis distance metric, p<0.01). We identified significant differences in enrichments of Bacteroides and Streptococcus species in cancer (all p<0.04). Folate (LDA 3.8) and arginine (LDA 4.1) were the most significantly altered pathways. We formed a novel microbiome-derived risk factor for prostate cancer based on 10 aberrant metabolic pathways (area under curve=0.64, p=0.02).
Conclusions: Microbiome analyses on men undergoing prostate biopsy noted mostly similar bacterial species diversity among men diagnosed with and without prostate cancer. The microbiome may have subtle influences on prostate cancer but are likely patient-specific and would require paired analysis and precise manipulation, such as improvement of natural bacterial folate production.
Patient summary: Microbiome evaluation may provide patients with personalized data regarding the presence or absence of particular bacteria that have metabolic functions and implications regarding prostate cancer risk. The study provides a basis to investigate the manipulation of aberrant microbiomes to reduce prostate cancer risk.
Keywords: B vitamins; Biomarker; Biotin; Folate; Microbiome; Prostate cancer.
Conflict of interest statement
Financial disclosures: Michael A. Liss certifies that all conflicts of interest, including specific financial interests and relationships and affiliations relevant to the subject matter or materials discussed in the manuscript (eg, employment/affiliation, grants or funding, consultancies, honoraria, stock ownership or options, expert testimony, royalties, or patents filed, received, or pending), are the following: James Robert White is the founder of Resphera Biosciences that performed the microbiome analysis but did not inform the statistical analysis of the biomarker component of the manuscript. Dr. White was paid as a consultant on the microbiome analysis.
Published by Elsevier B.V.
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Source: PubMed