The effect of low dose marine protein hydrolysates on short-term recovery after high intensity performance cycling: a double-blinded crossover study

Ingunn Mjøs, Einar Thorsen, Trygve Hausken, Einar Lied, Roy M Nilsen, Ingeborg Brønstad, Elisabeth Edvardsen, Bente Frisk, Ingunn Mjøs, Einar Thorsen, Trygve Hausken, Einar Lied, Roy M Nilsen, Ingeborg Brønstad, Elisabeth Edvardsen, Bente Frisk

Abstract

Background: Knowledge of the effect of marine protein hydrolysate (MPH) supplementation to promote recovery after high intensity performance training is scarce. The aim of this study was to examine the effect of MPH supplementation to whey protein (WP) and carbohydrate (CHO): (CHO-WP-MPH), on short-term recovery following high intensity performance, compared to an isoenergetic and isonitrogenous supplement of WP and CHO: (CHO-WP), in male cyclists.

Methods: This was a double-blinded crossover study divided into three phases. Fourteen healthy men participated. In phase I, an incremental bicycle exercise test was performed for establishment of intensities used in phase II and III. In phase II (9-16 days after phase 1), the participants performed first one high intensity performance cycling session, followed by nutrition supplementation (CHO-WP-MPH or CHO-WP) and 4 hours of recovery, before a subsequent high intensity performance cycling session. Phase III (1 week after phase II), was similar to phase II except for the nutrition supplementation, where the participants received the opposite supplementation compared to phase II. Primary outcome was difference in time to exhaustion between the cycling sessions, after nutrition supplementations containing MPH or without MPH. Secondary outcomes were differences in heart rate (HR), respiratory exchange ratio (RER), blood lactate concentration and glucose.

Results: The mean age of the participants was 45.6 years (range 40-58). The maximal oxygen uptake (mean ± SD) measured at baseline was 54.7 ± 4.1 ml∙min- 1∙kg- 1. There were no significant differences between the two nutrition supplementations measured by time to exhaustion at the cycling sessions (meandiff = 0.85 min, p = 0.156, 95% confidence interval (CI), - 0.37, 2.06), HR (meandiff = 0.8 beats pr.min, p = 0.331, 95% CI, - 0.9, 2.5), RER (meandiff = - 0.05, p = 0.361, 95% CI -0.07 - 0.17), blood lactate concentration (meandiff = - 0.24, p = 0.511, 95% CI, - 1.00, 0.53) and glucose (meandiff = 0.23, p = 0.094, 95% CI, - 0.05, 0.51).

Conclusions: A protein supplement with MPH showed no effects on short-term recovery in middle-aged healthy male cyclists compared to a protein supplement without MPH.

Trial registration: The study was registered 02.05.2017 at ClinicalTrials.gov (Protein Supplements to Cyclists, NCT03136133 , https://ichgcp.net/clinical-trials-registry/NCT03136133?cond=marine+peptides&rank=1 .

Keywords: Endurance exercise; Hydrolysed proteins; Marine protein hydrolysate; Recovery.

Conflict of interest statement

One of the co-authors, Einar Lied, was representing Firmenich Bjørge Biomarine A/S who was funding the study and responsible for composing the diets. To ensure that none of the authors were influenced if the diets contained marine protein hydrolysate or not, the study was double blinded, and the researchers who did the statistical analyses did not know anything about the order of the diets before all the analyses were completed and the results were given.

The other authors have no competing interests.

Figures

Fig. 1
Fig. 1
Flowchart of the study. V̇O2max: Maximal oxygen uptake
Fig. 2
Fig. 2
Mean difference in time between morning and afternoon cycling sessions. V̇O2max: maximal oxygen uptake; CHO: carbohydrate; WP: whey protein; MPH: marine protein hydrolysate

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