Influence of maternal age, gestational age and fetal gender on expression of immune mediators in amniotic fluid

Tobias Weissenbacher, Rüdiger P Laubender, Steven S Witkin, Andrea Gingelmaier, Barbara Schiessl, Franziskus Kainer, Klaus Friese, Udo Jeschke, Darius Dian, Katrin Karl, Tobias Weissenbacher, Rüdiger P Laubender, Steven S Witkin, Andrea Gingelmaier, Barbara Schiessl, Franziskus Kainer, Klaus Friese, Udo Jeschke, Darius Dian, Katrin Karl

Abstract

Background: Variations in cytokine and immune mediator expression patterns in amniotic fluid due to gestational age, maternal age and fetal gender were investigated.

Findings: Amniotic fluid samples were obtained from 192 women, 82 with a mid-trimester amniocentesis (median gestational age 17 weeks) and 110 with a caesarean section not in labor (median gestational age 39 weeks). Amniotic fluid was screened by commercial ELISAs for the TH1/TH2/TH17 cytokines and immune mediators IL-1 beta, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-15, IL-17, TNF alpha, GRO-alpha, MIP1alpha, MIP1beta, Histone, and IP10. Analysis was by Bonferroni correction for multiple comparisons. None of the 15 examined cytokines revealed any differences in expression patterns regarding fetal gender. Significant differences were found in IL-4, IL-10, IL-12, TNF- alpha, GRO-alpha and MIP1-beta with respect to gestational age and in GRO-alpha regarding maternal age.

Conclusion: Cytokines utilized as biomarkers in the diagnosis of intrauterine infections are not influenced in their expression pattern by fetal gender but may vary with respect to maternal age and gestational age.

References

    1. Dornbusch HJ, Strenger V, Kerbl R, Lackner H, Schwinger W, Sovinz P, Urban C. Procalcitonin and C-reactive protein do not discriminate between febrile reaction to anti-T-lymphocyte antibodies and Gram-negative sepsis. Bone Marrow Transplant. 2003;32(9):941–945. doi: 10.1038/sj.bmt.1704265.
    1. Prat C, Sancho JM, Dominguez J, Xicoy B, Gimenez M, Ferra C, Blanco S, Lacoma A, Ribera JM, Ausina V. Evaluation of procalcitonin, neopterin, C-reactive protein, IL-6 and IL-8 as a diagnostic marker of infection in patients with febrile neutropenia. Leuk Lymphoma. 2008;49(9):1752–1761. doi: 10.1080/10428190802258956.
    1. Bugatto F, Fernandez-Deudero A, Bailen A, Fernandez-Macias R, Hervias-Vivancos B, Bartha JL. Second-trimester amniotic fluid proinflammatory cytokine levels in normal and overweight women. Obstet Gynecol. 2010;115(1):127–133. doi: 10.1097/AOG.0b013e3181c5367f.
    1. Oboh AE, Orsi NM, Campbell J. Amniotic fluid cytokine profile in association with fetal hyperechogenic bowel. Eur J Obstet Gynecol Reprod Biol. 2006;128(1–2):86–90.
    1. Shohat B, Shohat M, Faktor JH, Barkay G, Harell D, Kozenitzky L. Soluble interleukin-2 receptor and interleukin-2 in human amniotic fluid of normal and abnormal pregnancies. Biol Neonate. 1993;63(5):281–284. doi: 10.1159/000243942.
    1. Kacerovsky M, Drahosova M, Hornychova H, Pliskova L, Bolehovska R, Forstl M, Tosner J, Andrys C. Value of amniotic fluid interleukin-8 for the prediction of histological chorioamnionitis in preterm premature rupture of membranes. Neuro Endocrinol Lett. 2009;30(6):733–738.
    1. Negishi H, Yamada H, Mikuni M, Kishida T, Okuyama K, Sagawa T, Makinoda S, Fujimoto S. Correlation between cytokine levels of amniotic fluid and histological chorioamnionitis in preterm delivery. J Perinat Med. 1996;24(6):633–639. doi: 10.1515/jpme.1996.24.6.633.
    1. Romero R, Avila C, Santhanam U, Sehgal PB. Amniotic fluid interleukin 6 in preterm labor. Association with infection. J Clin Invest. 1990;85(5):1392–1400. doi: 10.1172/JCI114583.
    1. Allbert JR, Naef RW 3rd, Perry KG Jr, Magann EF, Whitworth NS, Morrison JC. Amniotic fluid interleukin-6 and interleukin-8 levels predict the success of tocolysis in patients with preterm labor. J Soc Gynecol Investig. 1994;1(4):264–268.
    1. Heyborne KD, McGregor JA, Henry G, Witkin SS, Abrams JS. Interleukin-10 in amniotic fluid at midtrimester: immune activation and suppression in relation to fetal growth. Am J Obstet Gynecol. 1994;171(1):55–59.
    1. Velez DR, Fortunato SJ, Morgan N, Edwards TL, Lombardi SJ, Williams SM, Menon R. Patterns of cytokine profiles differ with pregnancy outcome and ethnicity. Hum Reprod. 2008;23(8):1902–1909. doi: 10.1093/humrep/den170.
    1. D'Alfonso A, Piancatelli D, Maccarone D, Adorno D, Iovenitti P, Carta G. Cytokine levels in amniotic fluid: a marker of preterm labor? Clin Exp Obstet Gynecol. 2006;33(1):34–35.
    1. Opsjln SL, Wathen NC, Tingulstad S, Wiedswang G, Sundan A, Waage A, Austgulen R. Tumor necrosis factor, interleukin-1, and interleukin-6 in normal human pregnancy. Am J Obstet Gynecol. 1993;169(2 Pt 1):397–404.
    1. Santhanam U, Avila C, Romero R, Viguet H, Ida N, Sakurai S, Sehgal PB. Cytokines in normal and abnormal parturition: elevated amniotic fluid interleukin-6 levels in women with premature rupture of membranes associated with intrauterine infection. Cytokine. 1991;3(2):155–163. doi: 10.1016/1043-4666(91)90037-E.
    1. Steinborn A, Gatje R, Kramer P, Kuhnert M, Halberstadt E. Cytokines in the diagnosis of amniotic infection syndrome. Z Geburtshilfe Perinatol. 1994;198(1):1–5.
    1. Underwood MA, Gilbert WM, Sherman MP. Amniotic fluid: not just fetal urine anymore. J Perinatol. 2005;25(5):341–348. doi: 10.1038/sj.jp.7211290.
    1. Wintour EM, Shandley L. Effects of fetal fluid balance on amniotic fluid volume. Semin Perinatol. 1993;17(3):158–172.
    1. Menon R, Camargo MC, Thorsen P, Lombardi SJ, Fortunato SJ. Amniotic fluid interleukin-6 increase is an indicator of spontaneous preterm birth in white but not black Americans. Am J Obstet Gynecol. 2008;198(1):77. e71-77.
    1. Menon R, Thorsen P, Vogel I, Jacobsson B, Morgan N, Jiang L, Li C, Williams SM, Fortunato SJ. Racial disparity in amniotic fluid concentrations of tumor necrosis factor (TNF)-alpha and soluble TNF receptors in spontaneous preterm birth. Am J Obstet Gynecol. 2008;198(5):533. e531-510.
    1. Menon R, Williams SM, Fortunato SJ. Amniotic fluid interleukin-1beta and interleukin-8 concentrations: racial disparity in preterm birth. Reprod Sci. 2007;14(3):253–259. doi: 10.1177/1933719107301336.
    1. Velez DR, Menon R, Thorsen P, Jiang L, Simhan H, Morgan N, Fortunato SJ, Williams SM. Ethnic differences in interleukin 6 (IL-6) and IL6 receptor genes in spontaneous preterm birth and effects on amniotic fluid protein levels. Ann Hum Genet. 2007;71(Pt 5):586–600.
    1. Bamberg C, Fotopoulou C, Linder M, Roehr CC, Dudenhausen JW, Henrich W, Kalache K. Mid-trimester amniotic fluid concentrations of the proinflammatory cytokines IL-6, IL-8, TNF-alpha, and lipopolysaccharide binding protein in normal pregnancies: a prospective evaluation according to parity, gestational age, and fetal gender. J Perinat Med. 2011;39(4):403–409.
    1. Chow SS, Craig ME, Jones CA, Hall B, Catteau J, Lloyd AR, Rawlinson WD. Differences in amniotic fluid and maternal serum cytokine levels in early midtrimester women without evidence of infection. Cytokine. 2008;44(1):78–84. doi: 10.1016/j.cyto.2008.06.009.
    1. Bry K, Lappalainen U, Waffarn F, Teramo K, Hallman M. Influence of fetal gender on the concentration of interleukin-1 receptor antagonist in amniotic fluid and in newborn urine. Pediatr Res. 1994;35(1):130–134. doi: 10.1203/00006450-199401000-00029.
    1. Romero R, Gomez R, Galasso M, Mazor M, Berry SM, Quintero RA, Cotton DB. The natural interleukin-1 receptor antagonist in the fetal, maternal, and amniotic fluid compartments: the effect of gestational age, fetal gender, and intrauterine infection. Am J Obstet Gynecol. 1994;171(4):912–921.
    1. Poggi SH, Spong CY, Ghidini A, Ossandon M. Gender differences in amniotic fluid cytokine levels. J Matern Fetal Neonatal Med. 2004;15(6):367–371. doi: 10.1080/14767050410001727396.
    1. Zicari A, Ticconi C, Pasetto N, Losardo A, Salerno A, Pontieri G, Piccone E. Interleukin-2 in human amniotic fluid during pregnancy and parturition: implications for prostaglandin E2 release by fetal membranes. J Reprod Immunol. 1995;29(3):197–208. doi: 10.1016/0165-0378(95)00945-H.
    1. Halgunset J, Johnsen H, Kjollesdal AM, Qvigstad E, Espevik T, Austgulen R. Cytokine levels in amniotic fluid and inflammatory changes in the placenta from normal deliveries at term. Eur J Obstet Gynecol Reprod Biol. 1994;56(3):153–160. doi: 10.1016/0028-2243(94)90162-7.

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