VEGFA rs2010963 GG genotype is associated with superior adaptations to resistance versus endurance training in the same group of healthy, young men

Maxime Boidin, Ellen A Dawson, Dick H J Thijssen, Robert M Erskine, Maxime Boidin, Ellen A Dawson, Dick H J Thijssen, Robert M Erskine

Abstract

Purpose: We used a within-subject, cross-over study to determine the relationship between the intra-individual adaptations to four weeks' resistance (RT) versus four weeks' endurance (END) training, and we investigated whether three single nucleotide polymorphisms (SNPs) were associated with these adaptations.

Methods: Thirty untrained, healthy, young men completed a cycling test to exhaustion to determine peak oxygen uptake (V̇O2peak), and a knee extension (KE) maximum voluntary isometric contraction (MVIC) of the right leg before and after four weeks' supervised RT (four sets of 10 repetitions at 80% single repetition maximum unilateral KE exercise, three times weekly) and four weeks' supervised END (30 min combined continuous/interval cycling, three times weekly), separated by a three-week washout phase. Participants were genotyped for the ACTN3 rs1815739, NOS3 rs2070744 and VEGFA rs2010963 SNPs.

Results: The intra-individual adaptations regarding percentage changes in MVIC force and V̇O2peak following RT and END, respectively, were unrelated (r2 = 0.003; P = 0.79). However, a VEGFA genotype × training modality interaction (P = 0.007) demonstrated that VEGFA GG homozygotes increased their MVIC force after RT (+ 20.9 ± 13.2%) more than they increased their V̇O2peak after END (+ 8.4 ± 9.1%, P = 0.005), and more than VEGFA C-allele carriers increased their MVIC force after RT (+ 12.2 ± 8.1%, P = 0.04). There were no genotype × training modality interactions for the ACTN3 or NOS3 SNPs.

Conclusion: High/low responders to RT were not consequently high/low responders to END or vice versa. However, preferential adaptation of VEGFA rs2010963 GG homozygotes to RT over END, and their greater adaptation to RT compared to VEGFA C-allele carriers, indicate a novel genetic predisposition for superior RT adaptation.

Keywords: Aerobic training; Genetic variation; Maximal force; Strength training; Training response.

Conflict of interest statement

The author declare that they have no conflict of interest.

© 2022. The Author(s).

Figures

Fig. 1
Fig. 1
Experimental design of the study. CPET cardiopulmonary exercise testing, MVIC maximum voluntary isometric contraction
Fig. 2
Fig. 2
Resistance (RT) and endurance (END) exercise training-induced changes (pre to post-RT and END) in knee extensor (KE) maximum voluntary isometric contraction (MVIC) force (A) and peak oxygen uptake (V̇O2peak) (B); *P < 0.05 post hoc paired t-test analyses (pre to post RT or END)
Fig. 3
Fig. 3
Individual percentage changes in knee extensor maximum voluntary isometric contraction (MVIC) force after resistance training (RT) compared to change in peak oxygen uptake (V̇O2peak) after endurance training (END) in all 30 individuals
Fig. 4
Fig. 4
Percentage changes in knee extensor maximum voluntary isometric contraction (MVIC) force after resistance training (RT) compared to change in peak oxygen uptake (V̇O2peak) after endurance training (END) in individuals of ACTN3 rs1815739 RR + RX and XX (A), NOS3 rs2070744 CC + TC and TT (B), and VEGFA rs2010963 CC + GC and GG (C) genotypes; *within-genotype difference (P < 0.05); # between-genotypes difference (P < 0.05)

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