Expression Patterns of Muscle-Specific miR-133b and miR-206 Correlate with Nutritional Status and Sarcopenia

Francesca Iannone, Alberto Montesanto, Erika Cione, Paolina Crocco, Maria Cristina Caroleo, Serena Dato, Giuseppina Rose, Giuseppe Passarino, Francesca Iannone, Alberto Montesanto, Erika Cione, Paolina Crocco, Maria Cristina Caroleo, Serena Dato, Giuseppina Rose, Giuseppe Passarino

Abstract

Sarcopenia and malnutrition are commonly occurring conditions in the elderly that frequently coexist, leading to substantial effects on morbidity/mortality. Evidence established muscle-specific microRNAs (miRNAs) or myomiRs as essential regulators of skeletal muscle processes, from myogenesis to muscle homeostasis. This study aimed to evaluate the association between myomiRs and sarcopenia and explore the potential of nutrition in mediating this association. qPCR was employed to characterize the myomiR-1, -133a/b, -206, -208b, and -499 expression profiles of 109 non-sarcopenic and 109 sarcopenic subjects. In our sample, the proportion malnourished or at-risk subjects was higher in sarcopenia (p < 0.001). Among the detected myomiRs (miR-133a/b and miR-206), lower levels of miR-133b was significantly associated with the presence of sarcopenia (p = 0.006); however, this relationship was not independent from nutritional status in multivariate analysis, suggesting a mediating effect of nutrition on the relationship between miR-133b and sarcopenia. Correlation analyses showed that lower miR-133b levels were associated with poor nutritional status (Mini Nutritional Assessment Long Form (MNA-LF) score, p = 0.005); furthermore, correlations with albumin, ferritin, and iron were found. Similar results were obtained for miR-206. Statistically more significant correlations were observed in subjects with sarcopenia. In conclusion, our findings highlight a nutrient-miR-133b/miR-206 pathway having a potential role in the age-related muscle decline.

Keywords: aging; miR-133b; miR-206; muscle wasting; myomiRs; nutritional status; sarcopenia.

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Relative miR-133b expression in plasma from sarcopenic and non-sarcopenic subjects. Data are reported as log 2−∆Ct normalized to U6 expression together with mean ± standard error of the mean (SEM) and p-value computed by t-test (p < 0.05).
Figure 2
Figure 2
Effect of nutritional status on plasma levels of (A) miR-133b and (B) miR-206 in sarcopenic and non-sarcopenic subjects. Data are reported as log 2−∆Ct normalized to U6 expression together with mean ± SEM and p-value computing by t-test (p < 0.05).
Figure 3
Figure 3
Correlations between plasma miR-133b levels and biochemical variables in sarcopenic and non-sarcopenic subjects. Scatter plots illustrate the relationship between plasma miR-133b levels and (A) albumin, (B) iron, (C) ferritin. Data are reported as log 2−∆Ct normalized to U6 expression.
Figure 4
Figure 4
Correlations between plasma miR-206 levels and biochemical variables in sarcopenic and non-sarcopenic subjects. Scatter plots illustrate the relationship between plasma miR-206 levels and (A) albumin, (B) ferritin. Data are reported as log 2−∆Ct normalized to U6 expression.

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