Curcumin Supplementation Decreases Intestinal Adiposity Accumulation, Serum Cholesterol Alterations, and Oxidative Stress in Ovariectomized Rats

Maurilio da Silva Morrone, Carlos Eduardo Schnorr, Guilherme Antônio Behr, Juciano Gasparotto, Rafael Calixto Bortolin, Katia da Boit Martinello, Bernardo Saldanha Henkin, Thallita Kelly Rabello, Alfeu Zanotto-Filho, Daniel Pens Gelain, José Cláudio Fonseca Moreira, Maurilio da Silva Morrone, Carlos Eduardo Schnorr, Guilherme Antônio Behr, Juciano Gasparotto, Rafael Calixto Bortolin, Katia da Boit Martinello, Bernardo Saldanha Henkin, Thallita Kelly Rabello, Alfeu Zanotto-Filho, Daniel Pens Gelain, José Cláudio Fonseca Moreira

Abstract

The aim of this study was to investigate the potential of curcumin oral supplementation (50 and 100 mg/Kg/day, for 30 days) in circumventing menopause-associated oxidative stress and lipid profile dysfunctions in a rat ovariectomy (OVX) model. Female Wistar rats were operated and randomly divided into either sham-operated or OVX groups. Sham-operated group (n = 8) and one OVX group (n = 11) were treated with vehicle (refined olive oil), and the other two OVX groups received curcumin at 50 or 100 mg/Kg/day doses (n = 8/group). OVX vehicle-treated animals presented a higher deposition of intestinal adipose tissue as well as increased serum levels of IL-6, LDL, and total cholesterol when compared to sham-operated rats. In addition, several oxidative stress markers in serum, blood, and liver (such as TBARS, carbonyl, reduced-sulphydryl, and nonenzymatic antioxidant defenses) were altered toward a prooxidant status by OVX. Interestingly, curcumin supplementation attenuated most of these parameters to sham comparable values. Thus, the herein presented results show that curcumin may be useful to ameliorate lipid metabolism alterations and oxidative damage associated with hormone deprivation in menopause.

Figures

Figure 1
Figure 1
Effects of ovariectomy and curcumin supplementation on serum (a) and liver (b) nonenzymatic antioxidant potential. An experiment's representative graphic and the area under curve of total reactive antioxidant potential were analyzed on both samples. Data are expressed by mean ± SEM (sham n = 8, OVX n = 11, OVX 50 n = 8, and OVX 100 n = 8) and the experiments were performed in triplicate. Statistical difference from sham group: bP < 0.05, cP < 0.01 (one-way ANOVA followed by the post hoc Tukey's test).
Figure 2
Figure 2
Effects of ovariectomy and curcumin supplementation on blood and liver antioxidant enzyme activities. Total superoxide dismutase (SOD) activity, catalase (CAT) activity, and glutathione peroxidase (GPx) activity were measured in blood (a) and liver (b) samples. Data are mean ± SEM (sham n = 8, OVX n = 11, OVX 50 n = 8, and OVX 100 n = 8) and the experiments were performed in triplicate. Statistical difference from sham group: bP < 0.05 (one-way ANOVA followed by the post hoc Tukey's test).
Figure 3
Figure 3
Effects of ovariectomy and curcumin supplementation on blood, liver, and serum thiol reduced content and oxidative damage markers. Proteic and nonproteic SH content, protein carbonyls groups, and lipid peroxidation were analyzed in blood (a), liver (b), and serum (c) samples. Data are expressed by mean ± SEM (sham n = 8, OVX n = 11, OVX 50 n = 8, and OVX 100 n = 8) and the experiments were performed in triplicate. Statistical difference from sham group: bP < 0.05, cP < 0.01. Statistical difference from OVX group: ∗P < 0.05, #P < 0.01 (one-way ANOVA followed by the post hoc Tukey's test).

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