Adherence to the Mediterranean diet assessed by a novel dietary biomarker score and mortality in older adults: the InCHIANTI cohort study

Nicole Hidalgo-Liberona, Tomás Meroño, Raul Zamora-Ros, Montserrat Rabassa, Richard Semba, Toshiko Tanaka, Stefania Bandinelli, Luigi Ferrucci, Cristina Andres-Lacueva, Antonio Cherubini, Nicole Hidalgo-Liberona, Tomás Meroño, Raul Zamora-Ros, Montserrat Rabassa, Richard Semba, Toshiko Tanaka, Stefania Bandinelli, Luigi Ferrucci, Cristina Andres-Lacueva, Antonio Cherubini

Abstract

Background: Dietary biomarkers may complement dietary intake assessment made by dietary questionnaires. We developed an a-posteriori dietary biomarkers score based on Mediterranean diet food groups and evaluated its association with mortality.

Methods: 642 participants (56% female), aged ≥65 years, with complete data on dietary biomarkers were followed during 20 years in the InCHIANTI cohort study (Tuscany, Italy). The main outcomes were all-cause, cardiovascular, and cancer mortality. Dietary biomarkers were selected from literature and from correlation analyses with dietary intakes of Mediterranean diet food groups in the study. The baseline levels of the following dietary biomarkers were chosen: urinary total polyphenols and resveratrol metabolites, and plasma carotenoids, selenium, vitamin B12, linolenic, eicosapentaenoic and docosahexaenoic acids, and the mono-unsaturated/saturated fatty acid ratio. Associations of the Mediterranean diet score using dietary biomarkers and a validated food frequency questionnaire (FFQ) (as tertiles) with mortality were assessed through Cox regression.

Results: During the 20-year follow-up [median (Q1-Q3), 14 (8-18) years], and 435 deaths occurred (139 from cardiovascular diseases and 89 from cancer-related causes). In the fully adjusted models, the dietary biomarker-Mediterranean diet score was inversely associated with all-cause (HRT3vs.T1 0.72; 95%CI 0.56-0.91) and cardiovascular (HRT3vs.T1 0.60; 95%CI 0.38-0.93), but not with cancer mortality. Associations between the FFQ-Mediterranean diet score and mortality were not statistically significant.

Conclusions: A greater adherence at baseline to a Mediterranean diet assessed by a dietary biomarker score was associated with a lower risk of mortality in older adults during a 20-year follow-up. The measurement of dietary biomarkers may contribute to guide individualized dietary counseling to older people.

Trial registration: NCT01331512.

Keywords: Carotenoids; Dietary biomarkers; Dietary questionnaires; Mediterranean diet; Mortality; Older adults; Polyphenols.

Conflict of interest statement

The authors declare that they have no competing interests.

© 2021. The Author(s).

Figures

Fig. 1
Fig. 1
Flowchart of participants of the study
Fig. 2
Fig. 2
Association between FFQ- and dietary biomarker-MDS and individual dietary biomarkers (as tertiles), and all-cause, CVD, and cancer mortality in the InCHIANTI Study. Cox regression model included sex, age, BMI, education, smoking status, physical activity, impaired renal function, diabetes mellitus, chronic obstructive pulmonary disease, hypertension, cardiovascular disease, cancer, dementia, Parkinson’s disease, and energy intake. FFQ food frequency questionnaire, dBMK dietary biomarker, EPA eicosapentaenoic acid, DHA docosahexaenoic acid, MUFA monounsaturated fatty acids, SFA saturated fatty acids. The total number of deaths, 435; CVD deaths, 139; cancer deaths, 85. Resveratrol was categorized into two groups: moderate vs. no or high consumers.

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