Scalp Hair Estradiol and Progesterone Level Determination in Women (Proof of Concept Study)
Scalp Hair Estradiol and Progesterone Level Determination in Pre-menopausal and Post-menopausal Women (Proof of Concept Study)
Studienübersicht
Status
Bedingungen
Detaillierte Beschreibung
One group will include 20 women under the age 40 years old, with regular menstruation cycles and without any illness or medication including birth control pills or steroidal ointments, and without known diagnosis of polycystic ovaries or fertility disorder. The second group will include 20 women over the age of 60, with no menstruation at least for the last five years, and without hormone therapy or treatment of any Steroidal therapy for the last year.
From all of the participants the investigators will collect hair samples at the crown back of the head (hair will be cut as close as possible to the scalp), the samples will be measured quantitatively about 100 hairs at a length of 2-3 cm. In order to grind the hair samples we will use a mini-Bead beater machine. milled hairs will be incubated overnight in a solution of 1 ml methanol 50, in constant gentle muddling. Afterwards, ethanol will disperse through a stream of nitrogen. The remainder of the suspension will be dissolved at 200 microliter buffer PBS (Phosphate-Buffered Saline) (pH 8.0) and the concentration of estrogen and progesterone will be measured by using the immune method which is available in the endocrine laboratory in Bnai Zion medical center. At the end of the study the investigators will cary out a statistical comparison of progesterone and estrogen levels between the two groups.
Studientyp
Einschreibung (Voraussichtlich)
Kontakte und Standorte
Studienorte
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Haifa, Israel, 31048
- Rekrutierung
- Samer Khoury
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Kontakt:
- Samer Khoury, MD
- Telefonnummer: 97248359100
- E-Mail: samer.khoury@b-zion.org.il
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Teilnahmekriterien
Zulassungskriterien
Studienberechtigtes Alter
Akzeptiert gesunde Freiwillige
Studienberechtigte Geschlechter
Probenahmeverfahren
Studienpopulation
Beschreibung
Inclusion Criteria:
- women under 40 with regular cycles
- Women over the age of 60 in menopause for at least 5 years
Exclusion Criteria:
- pregnant women
- oral contraceptive treatment in the last year
- hormonal replacement therapy in the last year
- 6 month after delivery
- infertile women
- irregular menstruation
- women with progesterone secretion intrauterine device in the last year
- BMI- Body Mass Index- below 25 or above 35
- Polycystic Ovarian Syndrome
- steriodal treatment including inhalers and ointments.
- status post Removal of the ovaries or uterus
- Breast or uterine cancer in the past.
- any pituitary or adrenal disease
Studienplan
Wie ist die Studie aufgebaut?
Designdetails
Kohorten und Interventionen
Gruppe / Kohorte |
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premenopausal and postmenopausal women
One group will include 20 premenopausal women under the age 40 years old, with regular menstruation cycles and without any illness or medication including birth control pills or steroidal ointments, and without known diagnosis of polycystic ovaries or fertility disorder.
The second group will include 20 postmenopausal women over the age of 60, with no menstruation at least for the last five years, and without hormone therapy or treatment of any Steroidal therapy for the last year.
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Was misst die Studie?
Primäre Ergebnismessungen
Ergebnis Maßnahme |
Maßnahmenbeschreibung |
Zeitfenster |
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concentration of hair scalp progesterone
Zeitfenster: 3 months
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concentration of hair scalp progesterone (nmol\L)
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3 months
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concentration of hair scalp estrogen
Zeitfenster: 3 months
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concentration of hair scalp estrogen (pmol\L)
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3 months
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Mitarbeiter und Ermittler
Sponsor
Ermittler
- Hauptermittler: samer khoury, Bnai Zion Medical Center
Studienaufzeichnungsdaten
Haupttermine studieren
Studienbeginn
Primärer Abschluss (Voraussichtlich)
Studienabschluss (Voraussichtlich)
Studienanmeldedaten
Zuerst eingereicht
Zuerst eingereicht, das die QC-Kriterien erfüllt hat
Zuerst gepostet (Schätzen)
Studienaufzeichnungsaktualisierungen
Letztes Update gepostet (Schätzen)
Letztes eingereichtes Update, das die QC-Kriterien erfüllt
Zuletzt verifiziert
Mehr Informationen
Begriffe im Zusammenhang mit dieser Studie
Schlüsselwörter
Andere Studien-ID-Nummern
- BNZ160015CTIL
Plan für individuelle Teilnehmerdaten (IPD)
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