Urinary Biopyrrins: A New Marker of Oxidative Stress in Psoriasis

Ola Ahmed Bakry, Sally El Hefnawy, Alaa Hassan Mariee, Yara El Gendy, Ola Ahmed Bakry, Sally El Hefnawy, Alaa Hassan Mariee, Yara El Gendy

Abstract

Background: Psoriasis is a common chronic, relapsing, immune-mediated disease involving skin and joints of genetically predisposed individuals. Oxidative stress has been found to play many important roles in cellular damage and loss of function in a number of tissues and organs and is believed to contribute to the pathogenesis of a variety of diseases. Urinary biopyrrin levels have gained attention as an indicator of oxidative stress.

Aim and objective: To measure urinary biopyrrins excretion as a marker of oxidative stress in psoriasis.

Patients and methods: This case-control study was carried out on 85 subjects; 55 cases with chronic plaque psoriasis and 30 age, gender and body mass index-matched normal subjects as a control group. Urinary biopyrrin levels were measured using enzyme immunoassay.

Results: There was a highly significant difference between cases and controls regarding urinary biopyrrins level (P < 0.001). There was significant positive correlation between biopyrrins level and both the age of cases (r = 0.28, P = 0.01) and psoriasis area and severity index score (r = 0.99, P < 0.001).

Conclusion: Urinary biopyrrins are increased in patients with psoriasis, and the level is correlated with disease severity. Further large-scale studies involving different ages and different clinical varieties of the disease are needed to expand and validate current findings. The clinical usefulness of antioxidants in psoriasis treatment needs to be evaluated in future research. Furthermore, the value of biopyrrins as biomarkers for monitoring response to therapy needs to be evaluated.

Keywords: Biopyrrins; enzyme immunoassay; oxidative stress; psoriasis.

Figures

Figure 1
Figure 1
(a) Biopyrrins levels in studied groups. (b) Significant positive correlation between urinary biopyrrins and age of studied cases. (c) Significant positive correlation between urinary biopyrrins and psoriasis area and severity index score of studied cases

References

    1. Lowes MA, Bowcock AM, Krueger JG. Pathogenesis and therapy of psoriasis. Nature. 2007;445:866–73.
    1. Menter A, Gottlieb A, Feldman SR, Van Voorhees AS, Leonardi CL, Gordon KB, et al. Guidelines of care for the management of psoriasis and psoriatic arthritis: Section 1. Overview of psoriasis and guidelines of care for the treatment of psoriasis with biologics. J Am Acad Dermatol. 2008;58:826–50.
    1. Jyothi RS, Govindswamy KS, Gurupadappa K. Psoriasis: An oxidative stress condition. J Clin Diagn Res. 2011;5:252–3.
    1. Pujari VM, Suryakar AN, Ireddy S. Oxidants and antioxidant status in psoriasis patients. Biomed Res. 2010;21:221–3.
    1. Gupta M, Chari S, Borkar M, Khede MC. Dyslipidemia and oxidative stress in patients of psoriasis. Biomed Res. 2011;22:221–4.
    1. Yamaguchi T, Shioji I, Sugimoto A, Komoda Y, Nakajima H. Epitope of 24G7 anti-bilirubin monoclonal antibody. Biochim Biophys Acta. 1996;1289:110–4.
    1. Chalmers RJ. Assessing psoriasis severity and outcomes for clinical trials and routine clinical practice. Dermatol Clin. 2015;33:57–71.
    1. Izumi Y, Yamazaki M, Shimizu S, Shimizu K, Yamaguchi T, Nakajima H. Anti-bilirubin monoclonal antibody. II. Enzyme-linked immunosorbent assay for bilirubin fractions by combination of two monoclonal antibodies. Biochim Biophys Acta. 1988;967:261–6.
    1. Stocker R, Yamamoto Y, McDonagh AF, Glazer AN, Ames BN. Bilirubin is an antioxidant of possible physiological importance. Science. 1987;235:1043–6.
    1. Yamaguchi T, Hashizume T, Tanaka M, Nakayama M, Sugimoto A, Ikeda S, et al. Bilirubin oxidation provoked by endotoxin treatment is suppressed by feeding ascorbic acid in a rat mutant unable to synthesize ascorbic acid. Eur J Biochem. 1997;245:233–40.
    1. Kozaki N, Shimizu S, Chijiiwa K, Yamaguchi K, Kuroki S, Shimoharada K, et al. Bilirubin as an anti-oxidant for surgical stress: A preliminary report of bilirubin oxidative metabolites. HPB Surg. 1999;11:241–8.
    1. Miyaoka T, Yasukawa R, Yasuda H, Shimizu M, Mizuno S, Sukegawa T, et al. Urinary excretion of biopyrrins, oxidative metabolites of bilirubin, increases in patients with psychiatric disorders. Eur Neuropsychopharmacol. 2005;15:249–52.
    1. Federico A, Morgillo F, Tuccillo C, Ciardiello F, Loguercio C. Chronic inflammation and oxidative stress in human carcinogenesis. Int J Cancer. 2007;121:2381–6.
    1. Corrocher R, Ferrari S, de Gironcoli M, Bassi A, Olivieri O, Guarini P, et al. Effect of fish oil supplementation on erythrocyte lipid pattern, malondialdehyde production and glutathione-peroxidase activity in psoriasis. Clin Chim Acta. 1989;179:121–31.
    1. Miyachi Y, Niwa Y. Effects of psoriatic sera on the generation of oxygen intermediates by normal polymorphonuclear leucocytes. Arch Dermatol Res. 1983;275:23–6.
    1. Turner CP, Toye AM, Jones OT. Keratinocyte superoxide generation. Free Radic Biol Med. 1998;24:401–7.
    1. Kadam DP, Suryakar AN, Ankush RD, Kadam CY, Deshpande KH. Role of oxidative stress in various stages of psoriasis. Indian J Clin Biochem. 2010;25:388–92.
    1. Padhi T, Garima Metabolic syndrome and skin: Psoriasis and beyond. Indian J Dermatol. 2013;58:299–305.
    1. Hunter HJ, Griffiths CE, Kleyn CE. Does psychosocial stress play a role in the exacerbation of psoriasis? Br J Dermatol. 2013;169:965–74.
    1. García-Bueno B, Caso JR, Leza JC. Stress as a neuroinflammatory condition in brain: Damaging and protective mechanisms. Neurosci Biobehav Rev. 2008;32:1136–51.
    1. Pajovic SB, Pejic S, Stojiljkovic V, Gavrilovic L, Dronjak S, Kanazir DT. Alterations in hippocampal antioxidant enzyme activities and sympatho-adrenomedullary system of rats in response to different stress models. Physiol Res. 2006;55:453–60.
    1. Hamzah MI. Oxidative stress markers (MDA, SOD and GSH) and proinflammatory ctokine (interleukine-18) in Iraqi patients with psoriasis vulgaris. Karbala J Med. 2014;7:1912–8.
    1. Chiba T, Tatematsu S, Nakao M, Furue M. Urinary biopyrrin: A potential inflammatory marker of atopic dermatitis. Ann Allergy Asthma Immunol. 2014;112:182–3.
    1. Hokamaki J, Kawano H, Yoshimura M, Soejima H, Miyamoto S, Kajiwara I, et al. Urinary biopyrrins levels are elevated in relation to severity of heart failure. J Am Coll Cardiol. 2004;43:1880–5.
    1. Kunii H, Ishikawa K, Yamaguchi T, Komatsu N, Ichihara T, Maruyama Y. Bilirubin and its oxidative metabolite biopyrrins in patients with acute myocardial infarction. Fukushima J Med Sci. 2009;55:39–51.
    1. Ito W, Tanigai T, Ueki S, Kayaba H, Chihara J. Increased urinary biopyrrins in patients with asthma. Allergy. 2010;65:1343–4.
    1. Ihara H, Matsumoto T, Kakinoki T, Morita Y, Takamiya K, Suzuki M. Urinary excretion of biopyrrin in unconjugated hyperbilirubinemia. J Anal Biosci. 2007;30:348–51.
    1. Suzuki M, Isonishi S, Morimoto O, Ogawa M, Ochiai K. Effect of sophrology on perinatal stress monitored by biopyrrin. Open J Obstet Gynecol. 2012;2:176–81.
    1. Yoshida R, Ogawa Y, Shioji I, Yu X, Shibata E, Mori I, et al. Urinary 8-oxo-7, 8-dihydro-2’-deoxyguanosine and biopyrrins levels among construction workers with asbestos exposure history. Ind Health. 2001;39:186–8.
    1. Pérez VI, Bokov A, Van Remmen H, Mele J, Ran Q, Ikeno Y, et al. Is the oxidative stress theory of aging dead? Biochim Biophys Acta. 2009;1790:1005–14.
    1. Gupta M, Chari S, Borkar M, Khede MC. Peroxisome proliferator-activated receptors (PPARs) and PPAR agonists: The ‘future’ in dermatologytherapeutics? Arch Dermatol Res. 2015
    1. Sertznig P, Seifert M, Tilgen W, Reichrath J. Peroxisome proliferator-activated receptors (PPARs) and the human skin: Importance of PPARs in skin physiology and dermatologic diseases. Am J Clin Dermatol. 2008;9:15–31.
    1. Millsop JW, Bhatia BK, Debbaneh M, Koo J, Liao W. Diet and psoriasis, part III: Role of nutritional supplements. J Am Acad Dermatol. 2014;71:561–9.
    1. Novío S, Núñez MJ, Ponte CM, Freire-Garabal M. Urinary biopyrrins: Potential biomarker for monitoring of the response to treatment with anxiolytics. Basic Clin Pharmacol Toxicol. 2012;111:206–10.
    1. Shimoharada K, Inoue S, Nakahara M, Kanzaki N, Shimizu S, Kang D, et al. Urine concentration of biopyrrins: A new marker for oxidative stress in vivo. Clin Chem. 1998;44:2554–5.
    1. Yamaguchi T, Shioji I, Sugimoto A, Komoda Y, Nakajima H. Chemical structure of a new family of bile pigments from human urine. J Biochem. 1994;116:298–303.
    1. Morita Y, Takahashi H, Kamihata H, Yamamoto Y, Hara K, Iwasaka T. Urinary excretion of biopyrrins, oxidative metabolites of bilirubin, increases after spasm provocation tests in patients with coronary spastic angina. Int J Cardiol. 2001;80:243–50.
    1. Yamaguchi T, Shioji I, Sugimoto A, Yamaoka M. Psychological stress increases bilirubin metabolites in human urine. Biochem Biophys Res Commun. 2002;293:517–20.
    1. Miyashita T, Yamaguchi T, Motoyama K, Unno K, Nakano Y, Shimoi K. Social stress increases biopyrrins, oxidative metabolites of bilirubin, in mouse urine. Biochem Biophys Res Commun. 2006;349:775–80.
    1. Irie M, Tsutsumi A, Shioji I, Kobayashi F. Effort-reward imbalance and physical health among Japanese workers in a recently downsized corporation. Int Arch Occup Environ Health. 2004;77:409–17.
    1. Aruoma OI, Cuppett SL. Champaign, IL: AOCS Press; 1997. Antioxidant Methodology: In vivo and In vitro concepts.

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