Molecular typing of nosocomial strains of Legionella pneumophila by arbitrarily primed PCR

F Grattard, P Berthelot, M Reyrolle, A Ros, J Etienne, B Pozzetto, F Grattard, P Berthelot, M Reyrolle, A Ros, J Etienne, B Pozzetto

Abstract

Arbitrarily primed PCR with two different primers was compared with ribotyping and monoclonal antibody analysis for typing Legionella strains. Applied to 11 epidemiologically unrelated strains, arbitrarily primed PCR resulted in an index of discrimination of 100% with both primers. It was found able to identify an epidemic clone of Legionella pneumophila serogroup 1 that was isolated from both patients and a hot water circuit of the same hospital.

References

    1. Infect Control Hosp Epidemiol. 1995 Apr;16(4):224-30
    1. J Clin Microbiol. 1995 Nov;33(11):2872-5
    1. J Clin Microbiol. 1994 Dec;32(12):2989-94
    1. J Clin Microbiol. 1995 Feb;33(2):402-6
    1. J Clin Microbiol. 1986 Apr;23(4):768-71
    1. J Infect Dis. 1988 Mar;157(3):486-95
    1. J Clin Microbiol. 1988 Nov;26(11):2465-6
    1. Epidemiol Infect. 1990 Apr;104(2):171-80
    1. J Infect Dis. 1992 Jul;166(1):22-30
    1. J Clin Microbiol. 1992 Jun;30(6):1491-8
    1. J Clin Microbiol. 1993 Jul;31(7):1940-2
    1. J Clin Microbiol. 1993 Aug;31(8):2198-200
    1. J Med Microbiol. 1994 Apr;40(4):275-81
    1. J Clin Microbiol. 1994 Mar;32(3):596-602
    1. J Hosp Infect. 1994 Mar;26(3):191-202
    1. Transpl Immunol. 1994;2(1):11-21

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