Association of bioavailable inhibin B and oocyte yield in controlled ovarian stimulation

Rachel B Danis, Intira Sriprasert, Jacqueline R Ho, Lynda K McGinnis, Ajay Kumar, Frank Z Stanczyk, Rachel B Danis, Intira Sriprasert, Jacqueline R Ho, Lynda K McGinnis, Ajay Kumar, Frank Z Stanczyk

Abstract

Objective: To determine if the biologically active or bioavailable inhibin B (bio-inhB) correlated with the oocyte yield in controlled ovarian stimulation (COS).

Design: Cross-sectional study.

Setting: Academic center.

Patients: Women undergoing oocyte cryopreservation.

Interventions: None.

Main outcome measures: Serum of women were sampled to measure bio-inhB at three points: baseline ("start"); middle ("mid"); and end of COS. A validated, highly specific enzyme-linked immunosorbent assay (Ansh Labs, Webster, TX) measured bio-inhB. The Spearman tests analyzed correlations between bio-inhB and other ovarian reserve markers, including age, follicle-stimulating hormone (FSH), antral follicle count (AFC), and antimüllerian hormone (AMH), and correlations between these markers and oocyte yield.

Results: A total of 144 women were included. Bioavailable inhibin B at the mid and end of COS, plus its delta, were strongly correlated with other ovarian reserve markers. As the bio-inhB concentration increased, the AFC and AMH levels also increased, whereas the FSH concentration and age decreased. Bioavailable inhibin B values, except at the start of COS, were more strongly correlated with oocyte yield than the FSH concentration (r = 0.72-0.82 vs. r = -0.44) and correlated similarly to the AFC and AMH concentration (r = 0.79 and 0.81, respectively). These correlations strengthened in those with diminished ovarian reserve, specifically age ≥35 years or AMH concentration <2 ng/mL (r = 0.71-0.86 vs. r = 0.49-0.67).

Conclusions: Predicting COS outcome is imperfect. When using a highly specific enzyme-linked immunosorbent assay, bio-inhB correlated with the oocyte yield similar to or more strongly than traditionally used ovarian reserve markers. These correlations strengthened in cases of diminished ovarian reserve. Bioavailable inhibin B provides physicians with an additional clinical tool for estimating COS outcome.

Keywords: Inhibin B; controlled ovarian stimulation; oocyte yield; ovarian reserve.

© 2021 The Author(s).

Figures

Figure 1
Figure 1
Trend of bioavailable inhibin B (bio-inhB) during controlled ovarian stimulation. Median concentration of bio-inhB at the start, middle, and end of stimulation; error bars indicate interquartile range. ∗n = 141; ∗∗n = 143.

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