Protective effects of dexmedetomidine on lung in rats with one-lung ventilation

Juntao Wang, Xuanlong Yi, Lili Jiang, He Dong, Wei Feng, Shuntao Wang, Chunqin Chu, Juntao Wang, Xuanlong Yi, Lili Jiang, He Dong, Wei Feng, Shuntao Wang, Chunqin Chu

Abstract

Protective effect of dexmedetomidine (DEX) on the lungs of one-lung ventilation (OLV) rat model and its effect on inflammatory factors were investigated. Ninety-two rats were selected and divided into groups A, B, C and D (n=23) according to the principle of similar body weight. OLV rat model was established. Before modeling (15 min), rats in group C were injected with sodium chloride. Rats in group D were injected with DEX at a speed of 5 µg/kg/h. Group A rats were ventilated in both lungs for 2 h. Rats in groups B and C (0.9% sodium chloride injection + OLV) and in group D (DEX + OLV) were subjected to OLV for 2 h and bilateral ventilation for 10 min. Concentrations of interleukin (IL)-6, IL-10 and tumor necrosis factor-α (TNF-α) in lung tissue of rats were detected by ELISA. The malondialdehyde (MDA) concentration and superoxide dismutase (SOD) activity in rat lung tissue were detected by radioimmunoassay. Wet weight (W)/dry weight (D) of lung tissue was calculated and indexes of the four groups of rats were compared. Compared with group A, IL-6, TNF-α and MDA concentrations and W/D of lung tissue of rats in groups B, C and D were significantly increased (p<0.05); SOD activity and IL-10 concentration were significantly decreased (p<0.01). Compared with groups B and C, the concentrations of IL-6, TNF-α and W/D in rats of group D were significantly decreased (p<0.01), but IL-10 significantly increased (p<0.01). Compared with groups B and C, the MDA concentration in lung tissue of rats in group D was significantly decreased (p<0.01), but SOD activity significantly increased (p<0.01). DEX can inhibit the production of inflammatory factors in the development and progression of pulmonary inflammation. It can inhibit lipid peroxidation, relieve pulmonary edema, and reduce lung injury after OLV, sin order to protect the lung.

Keywords: dexmedetomidine; lung injury; rat model; single-lung ventilation.

Figures

Figure 1.
Figure 1.
Comparison of lung tissue IL-6 concentration among four groups. ELISA double-antibody sandwich assay showed that the IL-6 concentration in the lungs of rats in groups B, C and D was significantly higher than that in group A. Compared with group B and C, IL-6 concentration in the lungs of rats in group D was significantly decreased. There was no significant difference in concentration of IL-6 between groups B and C (t=0.625, p=0.535). *P#p<0.001, compared with group D. IL, interleukin.
Figure 2.
Figure 2.
Comparison of lung tissue IL-10 concentration among four groups. ELISA double-antibody sandwich assay showed that, compared with group A, IL-10 concentration in the lung tissue of rats in groups B, C and D was significantly decreased. Compared with groups B and C, IL-10 concentration in the lungs of rats in group D increased significantly. There was no significant difference in IL-10 concentration between groups B and C (t=0.663, p=0.510). *P#p<0.001, compared with group D. IL, interleukin.
Figure 3.
Figure 3.
Comparison of TNF-α concentration among four groups. ELISA double-antibody sandwich assay showed that, compared with group A, TNF-α concentration in lung tissue of rats in groups B, C and D was significantly increased. Compared with groups B and C, the concentration of TNF-α in lung tissue of rats in group D was significantly decreased. There was no significant difference in the concentration of TNF-α between groups B and C (t=0.794, p=0.431). *P#p<0.001, compared with group D. TNF-α, tumor necrosis factor-α.
Figure 4.
Figure 4.
Comparison of MDA concentration in lung tissue of rats in four groups. Radioimmunoassay showed that, compared with group A, MDA concentration in lung tissue of rats in groups B, C and D was significantly increased. Compared with groups B and C, the MDA concentration in lung tissue of rats in group D was significantly decreased. There was no significant difference in concentration of MDA between groups B and C (t=0.525, p=0.601). *P#p<0.05, compared with group D. MDA, malondialdehyde.
Figure 5.
Figure 5.
Comparison of SOD activity in lung tissue of rats in four groups. Radioimmunoassay showed that, compared with group A, SOD activity in lung tissue of rats in groups B, C and D was significantly decreased. Compared with groups B and C, the SOD activity in lung tissue of rats in group D was significantly increased. There was no significant difference in SOD activity between groups B and C (t=0.734, p=0.466). *P#p<0.001, compared with group D. SOD, superoxide dismutase.
Figure 6.
Figure 6.
Comparison of lung tissue W/D among four groups. Compared with group A, W/D of lung tissue if rats in groups B, C and D was significantly increased. Compared with groups B and C, the W/D ratio of lung tissue of rats in group D was significantly decreased. There was no significant difference in lung tissue W/D between groups B and C (t=0.637, p=0.527). *P#p<0.05, compared with group D. W, wet weight; D, dry weight.

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