Clonal sequences recovered from plasma from patients with residual HIV-1 viremia and on intensified antiretroviral therapy are identical to replicating viral RNAs recovered from circulating resting CD4+ T cells
Jeffrey A Anderson, Nancie M Archin, William Ince, Daniel Parker, Ann Wiegand, John M Coffin, Joann Kuruc, Joseph Eron, Ronald Swanstrom, David M Margolis, Jeffrey A Anderson, Nancie M Archin, William Ince, Daniel Parker, Ann Wiegand, John M Coffin, Joann Kuruc, Joseph Eron, Ronald Swanstrom, David M Margolis
Abstract
Despite successful antiretroviral therapy (ART), low-level viremia (LLV) may be intermittently detected in most HIV-infected patients. Longitudinal blood plasma and resting CD4(+) T cells were obtained from two patients on suppressive ART to investigate the source of LLV. Single-genome sequencing of HIV-1 env from LLV plasma was performed, and the sequences were compared to sequences recovered from limiting-dilution outgrowth assays of resting CD4(+) T cells. The circulating LLV virus clone was identical to virus recovered from outgrowth assays from pools of millions of resting CD4(+) T cells. Understanding the sources of LLV requires evaluation of all possible reservoirs of persistent HIV infection.
Figures
Source: PubMed