Embryo development until blastocyst stage with and without renewal of single medium on day 3

Fernando Peña, Rocío Dávalos, Adolfo Rechkemmer, Alvaro Ascenzo, Mauricio Gonzales, Fernando Peña, Rocío Dávalos, Adolfo Rechkemmer, Alvaro Ascenzo, Mauricio Gonzales

Abstract

Objective: This study aimed to assess the effects of using a single culture medium not renewed on day 3 on the development of the embryo up to the blastocyst stage.

Methods: The study was carried out in the Assisted Reproduction Laboratory of Clínica Miraflores in the period ranging from April to December of 2016. The study included 589 human embryos obtained from 82 couples submitted to IVF/ICSI with donor oocytes. The couples were randomly divided into two groups: group 1 (medium renewed on day 3) and group 2 (medium not renewed on day 3).

Results: Significant differences in pregnancy rates, implantation rates, and blastocyst formation were not observed.

Conclusion: No statistically significant difference was found between the groups with respect to the analyzed parameters. However, a non-significant trend was observed in favor of the group without medium renewal for clinical pregnancy and positive pregnancy rates (β-hCG).

Keywords: Embryo culture; single medium; uninterrupted culture; with and without culture renewal.

Conflict of interest statement

CONFLICT OF INTEREST

No conflict of interest has been declared.

References

    1. Biggers JD, Racowsky C. The development of fertilized human ova to the blastocyst stage in KSOM(AA) medium: is a two-step protocol necessary? Reprod Biomed Online. 2002;5:133–140. doi: 10.1016/S1472-6483(10)61615-X.
    1. Biggers JD, Summers MC. Choosing a culture medium: making informed choices. Fertil Steril. 2008;90:473–483. doi: 10.1016/j.fertnstert.2008.08.010.
    1. Blake DA, Farquhar CM, Johnson N, Proctor M. Cleavage stage versus blastocyst stage embryo transfer in assisted conception. Cochrane Database Syst Rev. 2007;4:CD002118–CD002118. doi: 10.1002/14651858.CD002118.pub3.
    1. Cohen J, Gilligan A, Esposito W, Schimmel T, Dale B. Ambient air and its potential effects on conception in vitro. Hum Reprod. 1997;12:1742–1749. doi: 10.1093/humrep/12.8.1742.
    1. Costa-Borges N, Bellés M, Meseguer M, Galliano D, Ballesteros A, Calderón G. Blastocyst development in single medium with or without renewal on day 3: a prospective cohort study on sibling donor oocytes in a time-lapse incubator. Fertil Steril. 2016;105:707–713. doi: 10.1016/j.fertnstert.2015.11.038.
    1. Gardner DK, Lane M. Culture of viable human blastocysts in defined sequential serum-free media. Hum Reprod. 1998;13:148–159. doi: 10.1093/humrep/13.suppl_3.148.
    1. Hardy K, Hooper MA, Handyside AH, Rutherford AJ, Winston RM, Leese HJ. Non-invasive measurement of glucose and pyruvate uptake by individual human oocytes and preimplantation embryos. Hum Reprod. 1989;4:188–191. doi: 10.1093/oxfordjournals.humrep.a136869.
    1. Macklon NS, Pieters MH, Hassan MA, Jeucken PH, Eijkemans MJ, Fauser BC. A prospective randomized comparison of sequential versus monoculture systems for in-vitro human blastocyst development. Hum Reprod. 2002;17:2700–2705. doi: 10.1093/humrep/17.10.2700.
    1. Milki AA, Hinckley MD, Fisch JD, Dasig D, Behr B. Comparison of blastocyst transfer with day 3 embryo transfer in similar patient populations. Fertil Steril. 2000;73:126–129. doi: 10.1016/S0015-0282(99)00485-9.
    1. Rambhia P, Desai N. Global Medium Is Effective as a Single One-step Medium for Uninterrupted Culture to Blastocyst in the Embryscope: Preliminary Pregnancy and Clinical Outcome Data. Fertil Steril. 2014;101:e29. doi: 10.1016/j.fertnstert.2013.11.109.
    1. Reed ML, Hamic A, Thompson DJ, Caperton CL. Continuous uninterrupted single medium culture without medium renewal versus sequential media culture: a sibling embryo study. Fertil Steril. 2009;92:1783–1786. doi: 10.1016/j.fertnstert.2009.05.008.
    1. Sepúlveda S, Garcia J, Arriaga E, Diaz J, Noriega-Portella L, Noriega-Hoces L. In vitro development and pregnancy outcomes for human embryos cultured in either a single medium or in a sequential media system. Fertil Steril. 2009;91:1765–1770. DOI: 10.1016/j.fertnstert.2008.02.169.
    1. Sepúlveda SJ, Portella JR, Noriega LP, Escudero EL, Noriega LH. Extended culture up to the blastocyst stage: a strategy to avoid multiple pregnancies in assisted reproductive technologies. Biol Res. 2011;44:195–199. doi: 10.4067/S0716-97602011000200012.
    1. Velez de la Calle JF, Pfeffer J, Taar JP, Prigent Y. Blastocyst outcomes after sequential media culture vs single step media culture in a human IVF program. Fertil Steril. 2013;100:S253–S253. doi: 10.1016/j.fertnstert.2013.07.1192.
    1. Vermilyea M, Anthony J, Graham J, Tucker M. Op-3 Clinical Outcomes from an Uninterrupted Culture Medium Protocol. Reprod BioMed Online. 2012;24:S2–S2. doi: 10.1016/S1472-6483(12)60127-8.

Source: PubMed

Sponsorit ja yhteistyökumppanit

Sairaudet

Huumeiden interventiot

3
Tilaa