Effect of ultrasound-enhanced Nigella sativa seeds oil on wound healing: An animal model

Hany M Elgohary, Soad Kh Al Jaouni, Samy A Selim, Hany M Elgohary, Soad Kh Al Jaouni, Samy A Selim

Abstract

Objectives: Nigella sativa oil, ultrasound, and moist-exposed burn ointment (MEBO) have been suggested as noninvasive treatments for a number of inflammatory conditions and to accelerate wound healing. The aim of this study was to evaluate the efficiency of pulsed and continuous modes of ultrasound either alone or through phonophoresis, with N. sativa oil, or MEBO ointment in the treatment of chemical burns.

Methods: Thirty-five local rabbits were randomly divided into seven equal groups: pulsed ultrasound, continuous ultrasound, topical N. sativa oil, pulsed phonophoresis, continuous phonophoresis, topical MEBO ointment, and control group. Wound surface area was measured on days 0, 7, 14, and 21 using metric graph paper and photographs.

Results: Significant differences were found between pre- and post-treatment wounds in all groups, except for the control group, in favor of the pulsed phonophoresis. In contrast, low results were found in favor of continuous ultrasound.

Conclusion: Ultrasound, topical application of N. sativa oil, phonophoresis, and MEBO ointment have the potential to accelerate wound healing induced by chemical burns. Such treatment modalities may be used to treat wounds.

Keywords: Burn; Nigella sativa oil; Pulsed phonophoresis; Ultrasound; Wound.

References

    1. Jayasutha J., Monic J., Nithila S. Evaluation of wound healing activity of ethanolic extract of Aristolochia bracteata and Cassia tora on wistar albino rats. Int J Pharm Tech Res. 2011;3:1547–1550.
    1. Bjarnsholt T., Kirketerp-Møller K., Jensen P.Ø., Madsen K.G., Phipps R., Krogfelt K. Why chronic wounds won't heal: a novel hypothesis. Wound Repair Regen. 2008;1:2–10.
    1. Bishop A. Role of oxygen in wound healing. J Wound Care. 2008;17:399–402.
    1. Brem H., Tomic-Canic M. Cellular and molecular basis of wound healing in diabetes. J Clin Invest. 2007;117:1219–1222.
    1. Campos A., Groth A., Branco A. Assessment and nutritional aspects of wound healing. Curr Opin Clin Nutr Metab Care. 2008;11:281–288.
    1. Khan M.A., Chen H., Tania M., Zhang D. Anticancer activities of Nigella sativa (black cumin) Afr J Tradit, Complementary Altern Med. 2011;8:226–232.
    1. Chah K., Eze C., Emuelosi C., Esimone C. Antibacterial and wound healing properties of methanolic extracts of some Nigerian medicinal plants. J Ethnopharmacol. 2006;104:164–167.
    1. Atta M. Some characteristics of nigella (Nigella sativa L.) seed cultivated in Egypt and its lipid profile. Food Chem. 2003;83:63–68.
    1. Abu-Al-Basal M. Influence of Nigella sativa fixed oil on some blood parameters and histopathology of skin in staphylococcal-infected BALB/c mice. Pak J Biol Sci. 2011;14:1038–1046.
    1. Babayan V., Koottungal G., Halaby G. Proximate analysis, fatty acid and amino acid composition of Nigella sativa L. seeds. J Food Sci. 1978;43:1315–1319.
    1. Bamosa A., Basil A., Zubaida A. The effect of thymoquinone on blood lipids in rats. Indian J Pharmacol. 2002;46:195–201.
    1. El-Saleh S., Al-Saqair O., Al-Khalaf M. Thymoquinone and Nigella sativa oil protection against methionine-induced hyperhomocysteinemia in rats. Int J Cardiol. 2004;93:19–23.
    1. Gilani A., Jabeen Q., Khan M. A review of medicinal uses and pharmacological activities of Nigella sativa. Pak J Biol Sci. 2004;7:441–451.
    1. Watson T. Ultrasound in contemporary physiotherapy practice. Ultrasonics. 2008;48:321–329.
    1. Zhou S., Schmelz A., Seufferlein T., Li Y., Zhao J., Bachem M.G. Molecular mechanisms of low intensity pulsed ultrasound in human skin fibroblasts. J Biol Chem. 2004;52:54463–54469.
    1. Ang E.S., Lee S.T., Gan C.S., See P.I., Chan Y.H. The role of alternative therapy in the management of partial-thickness burns of the face; experience with use of moist exposed burn ointment (MEBO) compared with silver sulphadiazine. Ann Acad Med. 2000;29:7–10.
    1. Xu R. The medical science of burns and ulcers: a general introduction. Chin J Burns Wounds Surf Ulcers. 1989;1:11–21.
    1. Ioannovich J., Tsati E., Tsoutsos D., Frangia K., Papalois A. Moist exposed burn therapy: evaluation of the epithelial repair process (an experimental model) Ann Burns Fire Disasters. 2000;13:3–9.
    1. Dham R., Fathi A., Al Numairy A., Kadhim M., Wasielisky C., Shazly M. MEBO ointment in the treatment of burn wounds: a multicenter study. Mod Med. 1999;16:3–7.
    1. Divekar V., Naik L. Evolution of anaesthesia in India. JPGM. 2001;47(2):149–152.
    1. Bohannon R., Pfaller B. Documentation of wound surface area from tracings of wound perimeters. Clinical report on three techniques. Phys Ther. 1983;63:1622–1624.
    1. Shivananda N., Poorna N., Steve S., Vidyasagar B., Andrew A. Evaluation of wound healing activity of Allamandacathartica. L. and Laurusnobilis. L. extracts on rats. BMC Compl Alternative Med. 2006;6:12.
    1. Phillips G., Whitehead R., Knighton D. Initiation and pattern of angiogenesis in wound-healing in the rat. Am J Anat. 1991;192:257–262.
    1. Al-Bayaty F.H., Abdulla M.A., Abu Hassan M.I., Ali H.M. Effect of Andrographis paniculata leaf extract on wound healing in rats. Nat Prod Res. 2012;26:423–429.
    1. Ahmed I.H., Awad M.A., El-Mahdy M., Gohar H.M., Ghane A.M. The effect of some medicinal plant extracts on wound healing in farm animals. Assiut Vet Med J. 1995;32:236–244.
    1. Ab Rahman M., Abdul Razak F., Bakri M. Evaluation of wound closure activity of Nigella sativa, Melastoma malabathricum, Pluchea indica, and Piper sarmentosum extracts on scratched monolayer of human gingival fibroblasts. Evid Based Complement Altern Med. 2014:190342–190351.
    1. Ali B., Blunden G. Pharmacological and toxicological properties of nigella sativa. Phytother Res. 2003;17:299–305.
    1. Houghton P.J., Zarka R., Heras B., Hoult J.R. Fixed oil of Nigella sativa and derived thymoquinone inhibit eicosanoid generation in leuko cytes and membrane lipid peroxidation. Planta Med. 1995;61:33–46.
    1. Sharma N.K., Ahirwar D., Jhade D., Gupta S. Medicinal and pharmacological potential of Nigella sativa: a review. Ethnobot Rev. 2009;13:946–955.
    1. Boskabady M.H., Shirmohammadi B., Jandaghi P., Kiani S. Possible mechanism (s) for relaxant effect of aqueous and macerated extracts from Nigella sativa on tracheal chains of Guinea pig. BMC Pharmacol. 2004;4:3–8.
    1. Hosseinzadeh H., Parvardeh S., Asl M.N., Sadeghnia H.R., Ziaee T. Effect of thymoquinone and Nigella sativa seeds oil on lipid peroxidation level during global cerebral ischemia-reperfusion injury in rat hippocampus. Phytomedicine. 2007;14:621–627.
    1. Suboh S., Bilto Y., Aburjai T. Protective effects of selected medicinal plants against protein degradation, lipid peroxidation and deformability loss of oxidatively stressed human erythrocytes. Phytother Res. 2004;18:280–284.
    1. Ruthig D., Meckling-Gill K. Both (n-3) and (n-6) fatty acids stimulate wound in the rat intestinal epithelial cell line, IEC-6. J Nutr. 1999;129:1791–1798.
    1. Cambier D., Vanderstraeten G. Failure of therapeutic ultrasound in healing burn injuries. Burns. 1997;23:248–249.
    1. Loyd V., Nicholas G., Howard C. 9th ed. 2011. Ansel's pharmaceutical dosage forms and drug delivery system; pp. 300–303.
    1. Mitragotri S. Healing sound: the use of ultrasound in drug delivery and other therapeutic applications. Drug Discov. 2005;4:255–260.
    1. Ter R., Kessels A., Knipschild P. Randomized clinical trial of ultrasound in the treatment of pressure ulcers. Phys Ther. 1996;76:1301–1311.
    1. Smahel J. The problem of dehydration and healing of burn wounds. Burns. 1993;19:511–512.
    1. Atiyeh B.S., Al-Am C.A., El-Musa K.A., Dham R. The effect of moist exposed dressing: healing, barrier function and restoration of partial-thickness burn wounds. Eur J Plast Surg. 2003;26:5–11.
    1. Yeh G., Eisenberg D., Kaptchuk T. Systematic review of herbs and dietary supplements used in glycemic control in diabetes. Diabetes Care. 2003;26:1277–1294.

Source: PubMed

Sponsorit ja yhteistyökumppanit

Sairaudet

Huumeiden interventiot

3
Tilaa