SARS-CoV-2 is rapidly inactivated at high temperature

Jennifer Biryukov, Jeremy A Boydston, Rebecca A Dunning, John J Yeager, Stewart Wood, Allison Ferris, David Miller, Wade Weaver, Nathalie E Zeitouni, Denise Freeburger, Paul Dabisch, Victoria Wahl, Michael C Hevey, Louis A Altamura, Jennifer Biryukov, Jeremy A Boydston, Rebecca A Dunning, John J Yeager, Stewart Wood, Allison Ferris, David Miller, Wade Weaver, Nathalie E Zeitouni, Denise Freeburger, Paul Dabisch, Victoria Wahl, Michael C Hevey, Louis A Altamura

Abstract

In the absence of a vaccine, preventing the spread of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the primary means to reduce the impact of the 2019 coronavirus disease (COVID-19). Multiple studies have reported the presence of SARS-CoV-2 genetic material on surfaces suggesting that fomite transmission of SARS-CoV-2 is feasible. High temperature inactivation of virus has been previously suggested, but not shown. In the present study, we investigated the environmental stability of SARS-CoV-2 in a clinically relevant matrix dried onto stainless steel at a high temperature. The results show that at 54.5 °C, the virus half-life was 10.8 ± 3.0 min and the time for a 90% decrease in infectivity was 35.4 ± 9.0 min. These findings suggest that in instances where the environment can reach temperatures of at least 54.5 °C, such as in vehicle interior cabins when parked in warmer ambient air, that the potential for exposure to infectious virus on surfaces could be decreased substantially in under an hour.

Keywords: COVID-19; Coronavirus; Environmental decay; Heat; SARS-CoV-2; Viral inactivation.

Conflict of interest statement

Conflicts of interestThe authors declare no conflict of interest. DHS S & T staff performed a review of this manuscript prior to submission and provided editorial feedback.

© The Author(s) 2021.

Figures

Fig. 1
Fig. 1
Impact of temperature on the surface decay of the the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

References

    1. Ben-Shmuel A, Brosh-Nissimov T, Glinert I, Bar-David E, Sittner A, Poni R, Cohen R, Achdout H, Tamir H, Yahalom-Ronen Y, Politi B, Melamed S, Vitner E, Cherry L, Israeli O, Beth-Din A, Paran N, Israely T, Yitzhaki S, Levy H, Weiss S. Detection and infectivity potential of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) environmental contamination in isolation units and quarantine facilities. Clin Microbiol Infect. 2020;26(12):1658–1662. doi: 10.1016/j.cmi.2020.09.004.
    1. Biryukov J, Boydston JA, Dunning RA, Yeager JJ, Wood S, Reese AL, Ferris A, Miller D, Weaver W, Zeitouni NE, Phillips A, Freeburger D, Hooper I, Ratnesar-Shumate S, Yolitz J, Krause M, Williams G, Dawson DG, Herzog A, Dabisch P, Wahl V, Hevey MC, Altamura LA. Increasing temperature and relative humidity accelerates inactivation of SARS-CoV-2 on surfaces. mSphere. 2020 doi: 10.1128/mSphere.00441-20.
    1. Chia PY, Coleman KK, Tan YK, Ong SWX, Gum M, Lau SK, Lim XF, Lim AS, Sutjipto S, Lee PH, Son TT, Young BE, Milton DK, Gray GC, Schuster S, Barkham T, De PP, Vasoo S, Chan M, Ang BSP, Tan BH, Leo YS, Ng OT, Wong MSY, Marimuthu K, Singapore T, Novel Coronavirus Outbreak Research (2020) Detection of air and surface contamination by SARS-CoV-2 in hospital rooms of infected patients. Nat Commun 11(1): 2800 DOI: 10.1038/s41467-020-16670-2
    1. Chin AWH, Chu JTS, Perera MRA, Hui KPY, Yen HL, Chan MCW, Peiris M, Poon LLM. Stability of SARS-CoV-2 in different environmental conditions. Lancet Microbe. 2020;1(1):e10. doi: 10.1016/S2666-5247(20)30003-3.
    1. Ford Motor Company (2020) Packing heat: how ford’s latest tech helps police vehicles neutralize COVID-19. Retrieved from 25 Aug 2020
    1. Grundstein A, Meentemeyer V, Dowd J. Maximum vehicle cabin temperatures under different meteorological conditions. Int J Biometeorol. 2009;53(3):255–261. doi: 10.1007/s00484-009-0211-x.
    1. Guo ZD, Wang ZY, Zhang SF, Li X, Li L, Li C, Cui Y, Fu RB, Dong YZ, Chi XY, Zhang MY, Liu K, Cao C, Liu B, Zhang K, Gao YW, Lu B, Chen W. Aerosol and surface distribution of severe acute respiratory syndrome coronavirus 2 in hospital Wards, Wuhan, China, 2020. Emerg Infect Dis. 2020;26(7):1583–1591. doi: 10.3201/eid2607.200885.
    1. Horak J, Schmerold I, Wimmer K, Schauberger G. Cabin air temperature of parked vehicles in summer conditions: life-threatening environment for children and pets calculated by a dynamic model. Theoret Appl Climatol. 2016;130(1–2):107–118. doi: 10.1007/s00704-016-1861-3.
    1. Karber G. Beitrag zur kollektiven behandlung pharmakologischer reihenversuche. Archiv fur Experimentelle Pathologie und Pharmakologie. 1931;162:480–487. doi: 10.1007/BF01863914.
    1. Kasloff SB, Strong JE, Funk D, Cutts T. Stability of SARS-CoV-2 on critical personal protective equipment. medRxiv. 2020 doi: 10.1101/2020.06.11.20128884.
    1. McLaren C, Null J, Quinn J. Heat stress from enclosed vehicles: moderate ambient temperatures cause significant temperature rise in enclosed vehicles. Pediatrics. 2005;116(1):e109–112. doi: 10.1542/peds.2004-2368.
    1. Ong SWX, Tan YK, Chia PY, Lee TH, Ng OT, Wong MSY, Marimuthu K. Air, surface environmental, and personal protective equipment contamination by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from a symptomatic patient. JAMA. 2020;323(16):1610–1612. doi: 10.1001/jama.2020.3227.
    1. Ratnesar-Shumate S, Williams G, Green B, Krause M, Holland B, Wood S, Bohannon J, Boydston J, Freeburger D, Hooper I, Beck K, Yeager J, Altamura LA, Biryukov J, Yolitz J, Schuit M, Wahl V, Hevey M, Dabisch P. Simulated sunlight rapidly inactivates SARS-CoV-2 on surfaces. J Infect Dis. 2020;222(2):214–222. doi: 10.1093/infdis/jiaa274.
    1. Riddell S, Goldie S, Hill A, Eagles D, Drew TW. The effect of temperature on persistence of SARS-CoV-2 on common surfaces. Virol J. 2020;17(1):145. doi: 10.1186/s12985-020-01418-7.
    1. Santarpia JL, Rivera DN, Herrera VL, Morwitzer MJ, Creager HM, Santarpia GW, Crown KK, Brett-Major DM, Schnaubelt ER, Broadhurst MJ, Lawler JV, Reid SP, Lowe JJ. Aerosol and surface contamination of SARS-CoV-2 observed in quarantine and isolation care. Sci Rep. 2020;10(1):12732. doi: 10.1038/s41598-020-69286-3.
    1. Sharma VK, Jinadatha C, Lichtfouse E. Environmental chemistry is most relevant to study coronavirus pandemics. Environ Chem Lett. 2020 doi: 10.1007/s10311-020-01017-6.
    1. Spearman C. The method of ‘right and wrong cases’ (‘constant stimuli’) without Gauss's formulae. Br J Psychol. 1908;2(3):227–242.
    1. van Doremalen N, Bushmaker T, Morris DH, Holbrook MG, Gamble A, Williamson BN, Tamin A, Harcourt JL, Thornburg NJ, Gerber SI, Lloyd-Smith JO, de Wit E, Munster VJ. Aerosol and surface stability of SARS-CoV-2 as compared with SARS-CoV-1. N Engl J Med. 2020;382(16):1564–1567. doi: 10.1056/NEJMc2004973.
    1. Vanos JK, Middel A, Poletti MN, Selover NJ. Evaluating the impact of solar radiation on pediatric heat balance within enclosed, hot vehicles. Temperature (Austin) 2018;5(3):276–292. doi: 10.1080/23328940.2018.1468205.
    1. Wang X, Sun S, Zhang B, Han J. Solar heating to inactivate thermal-sensitive pathogenic microorganisms in vehicles: application to COVID-19. Environ Chem Lett. 2020 doi: 10.1007/s10311-020-01132-4.

Source: PubMed

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